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. 1970 Apr;117(3):525–531. doi: 10.1042/bj1170525

A variant of rabbit phosphoglucose isomerase

S G Welch 1, Linda I Fitch 1, C W Parr 1
PMCID: PMC1178956  PMID: 5419747

Abstract

An autosomally inherited variant of the rabbit enzyme phosphoglucose isomerase that differs both electrophoretically and kinetically from the usual (wild-type) rabbit enzyme has been investigated. Animals homozygous for this character have an isomerase with considerably decreased activity (in the erythrocytes), an increased Km for fructose 6-phosphate, an increased Ki for 6-phosphogluconate and a decreased stability towards heat and urea. The variant enzyme has been demonstrated in erythrocytes, leucocytes and tissues from the affected rabbits. The kinetic evidence suggests that the mutation present in the variant enzyme affects a histidine residue.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baughan M. A., Valentine W. N., Paglia D. E., Ways P. O., Simons E. R., DeMarsh Q. B. Hereditary hemolytic anemia associated with glucosephosphate isomerase (GPI) deficiency--a new enzyme defect of human erythrocytes. Blood. 1968 Aug;32(2):236–249. [PubMed] [Google Scholar]
  2. Carter N. D., Parr C. W. Isoenzymes of phosphoglucose isomerase in mice. Nature. 1967 Nov 4;216(5114):511–511. doi: 10.1038/216511a0. [DOI] [PubMed] [Google Scholar]
  3. DIXON M. The determination of enzyme inhibitor constants. Biochem J. 1953 Aug;55(1):170–171. doi: 10.1042/bj0550170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DIXON M. The effect of pH on the affinities of enzymes for substrates and inhibitors. Biochem J. 1953 Aug;55(1):161–170. doi: 10.1042/bj0550161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Detter J. C., Ways P. O., Giblett E. R., Baughan M. A., Hopkinson D. A., Povey S., Harris H. Inherited variations in human phosphohexose isomerase. Ann Hum Genet. 1968 May;31(4):329–338. doi: 10.1111/j.1469-1809.1968.tb00565.x. [DOI] [PubMed] [Google Scholar]
  6. Dyson J. E., Noltmann E. A. The effect of pH and temperature on the kinetic parameters of phosphoglucose isomerase. Participation of histidine and lysine in a proposed dual function mechanism. J Biol Chem. 1968 Apr 10;243(7):1401–1414. [PubMed] [Google Scholar]
  7. Fitch L. I., Parr C. W., Welch S. G. Phosphoglucose isomerase variation in man. Biochem J. 1968 Dec;110(4):56P–56P. doi: 10.1042/bj1100056pa. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KALOW W., GENEST K. A method for the detection of atypical forms of human serum cholinesterase; determination of dibucaine numbers. Can J Biochem Physiol. 1957 Jun;35(6):339–346. doi: 10.1139/y57-041. [DOI] [PubMed] [Google Scholar]
  9. NOLTMANN E. A. ISOLATION OF CRYSTALLINE PHOSPHOGLUCOSE ISOMERASE FROM RABBIT MUSCLE. J Biol Chem. 1964 May;239:1545–1550. [PubMed] [Google Scholar]
  10. Paglia D. E., Holland P., Baughan M. A., Valentine W. N. Occurrence of defective hexosephosphate isomerization in human erythrocytes and leukocytes. N Engl J Med. 1969 Jan 9;280(2):66–71. doi: 10.1056/NEJM196901092800203. [DOI] [PubMed] [Google Scholar]
  11. RAJAGOPAL M. V., SOHONIE K. Studies on the sea anemone Gyrostoma sp; lipids of Gyrostoma sp. Biochem J. 1957 Jan;65(1):34–36. doi: 10.1042/bj0650034. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SALAS M., VINUELA E., SOLS A. SPONTANEOUS AND ENZYMATICALLY CATALYZED ANOMERIZATION OF GLUCOSE 6-PHOSPHATE AND ANOMERIC SPECIFICITY OF RELATED ENZYMES. J Biol Chem. 1965 Feb;240:561–568. [PubMed] [Google Scholar]
  13. SILBER R., GABRIO B. W., HUENNEKENS F. M. Studies on normal and leukemic leukocytes. III. Pyridine nucleotides. J Clin Invest. 1962 Feb;41:230–234. doi: 10.1172/JCI104474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. TSUBOI K. K., ESTRADA J., HUDSON P. B. Enzymes of the human erythrocyte. IV. Phosphoglucose isomerase, purification and properties. J Biol Chem. 1958 Mar;231(1):19–29. [PubMed] [Google Scholar]
  15. WICK A. N., DRURY D. R., NAKADA H. I., WOLFE J. B. Localization of the primary metabolic block produced by 2-deoxyglucose. J Biol Chem. 1957 Feb;224(2):963–969. [PubMed] [Google Scholar]
  16. Yoshida A., Carter N. D. Nature of rabbit phosphoglucose isomerase isozymes. Biochim Biophys Acta. 1969 Nov 11;194(1):151–160. doi: 10.1016/0005-2795(69)90190-1. [DOI] [PubMed] [Google Scholar]

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