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. 1970 May;117(4):767–771. doi: 10.1042/bj1170767

The effect of colchicine on the transport of axonal protein in the chicken

K A C James 1, J J Bray 1,*, I G Morgan 1,†, L Austin 1
PMCID: PMC1179029  PMID: 4194591

Abstract

1. Small doses (1–10μg) of colchicine injected into the ventral horn of the spinal cord of the chicken caused paralysis in the legs. 2. Colchicine had no effect on the incorporation of leucine into proteins of the spinal cord but markedly decreased the total amount of protein flowing into the axons of the sciatic nerve. 3. This axonal flow of protein proceeded at two rates: a high rate (300mm/day) and a low rate (2mm/day). Although both groups of proteins were affected, the slow transport of protein was more profoundly blocked by colchicine. 4. The results suggest that axonal flow is dependent on the neurotubular system in the axon.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Austin L., Bray J. J., Young R. J. Transport of proteins and ribonucleic acid along nerve axons. J Neurochem. 1966 Nov;13(11):1267–1269. doi: 10.1111/j.1471-4159.1966.tb04288.x. [DOI] [PubMed] [Google Scholar]
  2. Bray J. J., Austin L. Axoplasmic transport of 14C proteins at two rates in chicken sciatic nerve. Brain Res. 1969 Jan;12(1):230–233. doi: 10.1016/0006-8993(69)90069-9. [DOI] [PubMed] [Google Scholar]
  3. Buckley I. K., Porter K. R. Cytoplasmic fibrils in living cultured cells. A light and electron microscope study. Protoplasma. 1967;64(4):349–380. doi: 10.1007/BF01666538. [DOI] [PubMed] [Google Scholar]
  4. DROZ B., LEBLOND C. P. AXONAL MIGRATION OF PROTEINS IN THE CENTRAL NERVOUS SYSTEM AND PERIPHERAL NERVES AS SHOWN BY RADIOAUTOGRAPHY. J Comp Neurol. 1963 Dec;121:325–346. doi: 10.1002/cne.901210304. [DOI] [PubMed] [Google Scholar]
  5. Dahlström A., Häggendal J. Studies on the transport and life-span of amine storage granules in the adrenergic neuron system of the rabbit sciatic nerve. Acta Physiol Scand. 1967 Mar;69(3):153–157. doi: 10.1111/j.1748-1716.1967.tb03506.x. [DOI] [PubMed] [Google Scholar]
  6. GEIGER R. S. THE BEHAVIOR OF ADULT MAMMALIAN BRAIN CELLS IN CULTURE. Int Rev Neurobiol. 1963;5:1–52. doi: 10.1016/s0074-7742(08)60593-0. [DOI] [PubMed] [Google Scholar]
  7. Kreutzberg G. W. Neuronal dynamics and axonal flow. IV. Blockage of intra-axonal enzyme transport by colchicine. Proc Natl Acad Sci U S A. 1969 Mar;62(3):722–728. doi: 10.1073/pnas.62.3.722. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Livett B. G., Geffen L. B., Austin L. Proximo-distal transport of [14C]noradrenaline and protein in sympathetic nerves. J Neurochem. 1968 Sep;15(9):931–939. doi: 10.1111/j.1471-4159.1968.tb11635.x. [DOI] [PubMed] [Google Scholar]
  10. McEwen B. S., Grafstein B. Fast and slow components in axonal transport of protein. J Cell Biol. 1968 Sep;38(3):494–508. doi: 10.1083/jcb.38.3.494. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ochs S., Johnson J., Ng M. H. Protein incorporation and axoplasmic flow in motoneuron fibres following intra-cord injection of labelled leucine. J Neurochem. 1967 Mar;14(3):317–331. doi: 10.1111/j.1471-4159.1967.tb09529.x. [DOI] [PubMed] [Google Scholar]
  12. Rosenbaum J. L., Carlson K. Cilia regeneration in Tetrahymena and its inhibition by colchicine. J Cell Biol. 1969 Feb;40(2):415–425. doi: 10.1083/jcb.40.2.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schmitt F. O. Fibrous proteins--neuronal organelles. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1092–1101. doi: 10.1073/pnas.60.4.1092. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tilney L. G. Studies on the microtubules in heliozoa. IV. The effect of colchicine on the formation and maintenance of the axopodia and the redevelopment of pattern in Actinosphaerium nucleofilum (Barrett). J Cell Sci. 1968 Dec;3(4):549–562. doi: 10.1242/jcs.3.4.549. [DOI] [PubMed] [Google Scholar]
  15. Wisniewski H., Shelanski M. L., Terry R. D. Effects of mitotic spindle inhibitors on neurotubules and neurofilaments in anterior horn cells. J Cell Biol. 1968 Jul;38(1):224–229. doi: 10.1083/jcb.38.1.224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wiśniewski H., Terry R. D. Experimental colchicine encephalopathy. I. Induction of neurofibrillary degeneration. Lab Invest. 1967 Dec;17(6):577–587. [PubMed] [Google Scholar]

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