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. 1970 May;117(4):791–797. doi: 10.1042/bj1170791

The oxidation of reduced nicotinamide nucleotides by hydrogen peroxide in the presence of lactoperoxidase and thiocyanate, iodide or bromide

D McC Hogg 1, G R Jago 1
PMCID: PMC1179032  PMID: 4317722

Abstract

Lactoperoxidase (EC 1.11.1.7) catalysed the oxidation of NADH by hydrogen peroxide in the presence of either thiocyanate, iodide or bromide. In the presence of thiocyanate, net oxidation of thiocyanate occurred simultaneously with the oxidation of NADH, but in the presence of iodide or bromide, only the oxidation of NADH occurred to a significant extent. In the presence of thiocyanate or bromide, NADH was oxidized to NAD+ but in the presence of iodide, an oxidation product with spectral and chemical properties distinct from NAD+ was formed. Thiocyanate, iodide and bromide appeared to function in the oxidation of NADH by themselves being oxidized to products which in turn oxidized NADH, rather than by activating the enzyme. Iodine, which oxidized NADH non-enzymically, appeared to be an intermediate in the oxidation of NADH in the presence of iodide. NADPH was oxidized similarly under the same conditions. An assessment was made of the rates of these oxidation reactions, together with the rates of other lactoperoxidase-catalysed reactions, at physiological concentrations of thiocyanate, iodide and bromide. The results indicated that in milk and saliva the oxidation of thiocyanate to a bacterial inhibitor was likely to predominate over the oxidation of NADH.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AKAZAWA T., CONN E. E. The oxidation of reduced pyridine nucleotides by peroxidase. J Biol Chem. 1958 May;232(1):403–415. [PubMed] [Google Scholar]
  2. ALEXANDER N. M. A spectrophotometric assay for iodide oxidation by thyroid peroxidase. Anal Biochem. 1962 Oct;4:341–345. doi: 10.1016/0003-2697(62)90097-0. [DOI] [PubMed] [Google Scholar]
  3. ALEXANDER N. M. The mechanism of iodination reactions in thyroid glands. Endocrinology. 1961 Apr;68:671–679. doi: 10.1210/endo-68-4-671. [DOI] [PubMed] [Google Scholar]
  4. BOULANGE M., HAN K., VERT P. FLUCTUATION SAISONNI'ERE DES THIOCYANATES DANS UN LAIT INDUSTRIEL. (OBSERVATIONS SUR CINQ ANN'EES CONS'ECUTIVES) C R Seances Soc Biol Fil. 1963 Oct 5;157:1074–1076. [PubMed] [Google Scholar]
  5. BOULANGE M. [Seasonal fluctuations in the thiocyanate level in fresh cow's milk]. C R Seances Soc Biol Fil. 1959;153:2019–2020. [PubMed] [Google Scholar]
  6. COLOWICK S. P., KAPLAN N. O., CIOTTI M. M. The reaction of pyridine nucleotide with cyanide and its analytical use. J Biol Chem. 1951 Aug;191(2):447–459. [PubMed] [Google Scholar]
  7. De Duve C., Baudhuin P. Peroxisomes (microbodies and related particles). Physiol Rev. 1966 Apr;46(2):323–357. doi: 10.1152/physrev.1966.46.2.323. [DOI] [PubMed] [Google Scholar]
  8. Densen P. M., Davidow B., Bass H. E., Jones E. W. A chemical test for smoking exposure. Arch Environ Health. 1967 Jun;14(6):865–874. doi: 10.1080/00039896.1967.10664853. [DOI] [PubMed] [Google Scholar]
  9. FLETCHER K., HONOUR A. J., ROWLANDS E. N. Studies on the concentration of radioiodide and thiocyanate by slices of the salivary gland. Biochem J. 1956 Jun;63(2):194–199. doi: 10.1042/bj0630194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. FREINKEL N., INGBAR S. H. The metabolism of I131 by surviving slices of rat mammary tissue. Endocrinology. 1956 Jan;58(1):51–56. doi: 10.1210/endo-58-1-51. [DOI] [PubMed] [Google Scholar]
  11. HOLLANDER V. P., STEPHENS M. L. Studies on phenol-activated oxidation of reduced nucleotides by rat uterus. J Biol Chem. 1959 Jul;234(7):1901–1906. [PubMed] [Google Scholar]
  12. Hogg D. M., Jago G. R. The antibacterial action of lactoperoxidase. The nature of the bacterial inhibitor. Biochem J. 1970 May;117(4):779–790. doi: 10.1042/bj1170779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. KLEBANOFF S. J., LUEBKE R. G. THE ANTILACTOBACILLUS SYSTEM OF SALIVA. ROLE OF SALIVARY PEROXIDASE. Proc Soc Exp Biol Med. 1965 Feb;118:483–486. doi: 10.3181/00379727-118-29882. [DOI] [PubMed] [Google Scholar]
  14. KLEBANOFF S. J., YIP C., KESSLER D. The iodination of tyrosine by beef thyroid preparations. Biochim Biophys Acta. 1962 Apr 23;58:563–574. doi: 10.1016/0006-3002(62)90067-7. [DOI] [PubMed] [Google Scholar]
  15. Klebanoff S. J. Iodination of bacteria: a bactericidal mechanism. J Exp Med. 1967 Dec 1;126(6):1063–1078. doi: 10.1084/jem.126.6.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LOGOTHETOPOULOS J. H., MYANT N. B. Concentration of radio-iodide and 35-S-thiocyanate by the salivary glands. J Physiol. 1956 Oct 29;134(1):189–194. doi: 10.1113/jphysiol.1956.sp005634. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. McQUILLAN M. T., MORTON R. K., STANLEY P. G., TRIKOJUS V. M. Enzymic iodination of milk proteins. Nature. 1954 Feb 13;173(4398):305–307. doi: 10.1038/173305b0. [DOI] [PubMed] [Google Scholar]
  18. Oram J. D., Reiter B. The inhibition of streptococci by lactoperoxidase, thiocyanate and hydrogen peroxide. The effect of the inhibitory system on susceptible and resistant strains of group N streptococci. Biochem J. 1966 Aug;100(2):373–381. doi: 10.1042/bj1000373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Oram J. D., Reiter B. The inhibition of streptococci by lactoperoxidase, thiocyanate and hydrogen peroxide. The oxidation of thiocyanate and the nature of the inhibitory compound. Biochem J. 1966 Aug;100(2):382–388. doi: 10.1042/bj1000382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. POTTER G. D., TONG W., CHAIKOFF I. L. The metabolism of I 131-labeled iodine, thyroxine, and triiodothyronine in the mammary gland of the lactating rat. J Biol Chem. 1959 Feb;234(2):350–354. [PubMed] [Google Scholar]
  21. Slowey R. R., Eidelman S., Klebanoff S. J. Antibacterial activity of the purified peroxidase from human parotid saliva. J Bacteriol. 1968 Sep;96(3):575–579. doi: 10.1128/jb.96.3.575-579.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. ULLBERG S., APPELGREN L. E., CLEMEDSON C. J., ERICSSON Y., EWALDSSON B., SOERBO B., SOEREMARK R. A COMPARISON OF THE DISTRIBUTION OF SOME HALIDE IONS IN THE BODY. Biochem Pharmacol. 1964 Mar;13:407–412. doi: 10.1016/0006-2952(64)90160-1. [DOI] [PubMed] [Google Scholar]
  23. WOKES F., WEDGWOOD P., WYATT J. Thiocyanates in milk and other biological fluids. Biochem J. 1952 Jan;50(3):xix–xx. [PubMed] [Google Scholar]
  24. WOLFF J., MAUREY J. R. Thyroidal iodide transport. II. Comparison with non-thyroid iodide-concentrating tissues. Biochim Biophys Acta. 1961 Mar 4;47:467–474. doi: 10.1016/0006-3002(61)90542-x. [DOI] [PubMed] [Google Scholar]
  25. ZELDOW B. J. Studies on the antibacterial action of human saliva. III. Cofactor requirements of Lactobacillus bactericidin. J Immunol. 1963 Jan;90:12–16. [PubMed] [Google Scholar]

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