Abstract
Electrophoresis in a sucrose gradient at pH values between 5 and 8 separated housefly DDT [1,1,1-trichloro-2,2-bis-(p-chlorophenyl)ethane] dehydrochlorinase into two major fractions. GSH S-aryltransferase under similar conditions migrated as a single peak of activity. Separation of housefly homogenates or partially purified enzyme preparations by electrofocusing in a natural pH gradient also showed the presence of multiple forms of DDT dehydrochlorinase.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Balabaskaran S., Smith J. N. The inhibition of 1,1,1-trichloro-2,2-bis-(p-chlorophenyl)ethane (DDT) dehydrochlorinase and glutathione S-aryltransferase in grass-grub and housefly preparations. Biochem J. 1970 May;117(5):989–996. doi: 10.1042/bj1170989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Booth J., Boyland E., Sims P. An enzyme from rat liver catalysing conjugations with glutathione. Biochem J. 1961 Jun;79(3):516–524. doi: 10.1042/bj0790516. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Carlström A., Vesterberg O. Isoelectric focusing and separation of the subcomponents of lactoperoxidase. Acta Chem Scand. 1967;21(1):271–278. doi: 10.3891/acta.chem.scand.21-0271. [DOI] [PubMed] [Google Scholar]
- Clark A. G., Darby F. J., Smith J. N. Species differences in the inhibition of glutathione S-aryltransferase by phthaleins and dicarboxylic acids. Biochem J. 1967 Apr;103(1):49–54. doi: 10.1042/bj1030049. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DIXON M. The determination of enzyme inhibitor constants. Biochem J. 1953 Aug;55(1):170–171. doi: 10.1042/bj0550170. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Flatmark T. On the heterogeneity of beef heart cytochrome c. II. Some physico-chemical properties of the main subfractions (Cy I-Cy 3). Acta Chem Scand. 1966;20(6):1476–1486. doi: 10.3891/acta.chem.scand.20-1476. [DOI] [PubMed] [Google Scholar]
- LIPKE H., KEARNS C. W. DDT dehydrochlorinase. I. Isolation, chemical properties, and spectrophotometric assay. J Biol Chem. 1959 Aug;234(8):2123–2128. [PubMed] [Google Scholar]
- Vesterberg O., Svensson H. Isoelectric fractionation, analysis, and characterization of ampholytes in natural pH gradients. IV. Further studies on the resolving power in connection with separation of myoglobins. Acta Chem Scand. 1966;20(3):820–834. doi: 10.3891/acta.chem.scand.20-0820. [DOI] [PubMed] [Google Scholar]