Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1970 Jun;118(1):67–70. doi: 10.1042/bj1180067

Purification and crystallization of avidin

N M Green 1, E J Toms 1
PMCID: PMC1179080  PMID: 5528638

Abstract

An improved purification, including crystallization, of avidin from hen's-egg white is described. The product bound 15.1μg of biotin/mg, corresponding to an equivalent weight of 16200. The amino acid composition showed an integral number of amino acid residues per 16200g, which suggested that each molecule of avidin (mol. wt. approx. 66000) contained four identical subunits.

Full text

PDF
67

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CRUMPTON M. J., WILKINSON J. M. AMINO ACID COMPOSITIONS OF HUMAN AND RABBIT GAMMA-GLOBULINS AND OF THE FRAGMENTS PRODUCED BY REDUCTION. Biochem J. 1963 Aug;88:228–234. doi: 10.1042/bj0880228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. FRAENKEL-CONRAT H., SNELL N. S., DUCAY E. D. Avidin. I. Isolation and characterization of the protein and nucleic acid. Arch Biochem Biophys. 1952 Jul;39(1):80–96. doi: 10.1016/0003-9861(52)90263-4. [DOI] [PubMed] [Google Scholar]
  3. Green N. M. Evidence for a genetic relationship between avidins and lysozymes. Nature. 1968 Jan 20;217(5125):254–256. doi: 10.1038/217254a0. [DOI] [PubMed] [Google Scholar]
  4. Green N. M., Joynson M. A. A preliminary crystallographic investigation of avidin. Biochem J. 1970 Jun;118(1):71–72. doi: 10.1042/bj1180071. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Green N. M., Ross M. E. The effect of N-bromosuccinimide on the sub-unit structure of acidin and its complexes with biotin. Biochem J. 1968 Nov;110(1):59–66. doi: 10.1042/bj1100059. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kup W., Lange D. Zu den bösartigen Geschwülsten in der Hals-Nasen-Ohren-Heilkunde. II. Tumoren der Nase und ihrer Nebenhöhlen. 2. Behandlungsmethoden, Ergebnisse, Probleme der Nachsorge und Rehabilitation. Arch Geschwulstforsch. 1970;35(2):129–145. [PubMed] [Google Scholar]
  7. MELAMED M. D., GREEN N. M. AVIDIN. 2. PURIFICATION AND COMPOSITION. Biochem J. 1963 Dec;89:591–599. doi: 10.1042/bj0890591. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES