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. 1970 Jun;118(1):93–97. doi: 10.1042/bj1180093

Uptake of oestradiol by the rabbit hypothalamus. Specificity of binding by nuclei in vitro

Gerald J Chader 1,*, Claude A Villee 1
PMCID: PMC1179085  PMID: 5472158

Abstract

The binding of [6,7-3H]oestradiol-17β to subcellular fractions of the hypothalamus and the cerebellum of the rabbit was studied in vitro. Uptake of steroid was higher in hypothalamic nuclei than in cerebellar nuclei. Lower binding was observed in other fractions of both tissues. After dialysis of the fractions, hypothalamic nuclei retained a high percentage of oestradiol whereas cerebellar nuclei lost most of the bound steroid. Supernatant fractions of both tissues retained a significant proportion of label after dialysis and after gel filtration on Sephadex G-200. No specific binding was observed in these fractions when subjected to sucrose-density-gradient centrifugation. Purification of nuclei followed by incubation with labelled oestradiol in the absence of the supernatant fraction resulted in loss of binding of steroid by hypothalamic nuclei. Incubation of the purified hypothalamic nuclei with supernatant fraction maintained the binding specificity of hormone retention.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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