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. 1970 Jun;118(2):201–208. doi: 10.1042/bj1180201

Oxygenation of methane by methane-grown Pseudomonas methanica and Methanomonas methanooxidans

I J Higgins 1, J R Quayle 1
PMCID: PMC1179104  PMID: 5484663

Abstract

1. Experimental conditions have been found in which small amounts of methanol (approximately 2.5mm) accumulated when washed cell suspensions of methane-grown Pseudomonas methanica and Methanomonas methanooxidans were incubated with methane+oxygen mixtures in Warburg flasks. 2. The methanol formed could be separated completely from water by fractional distillation through glass helices followed by gas chromatography using 20% polyethylene glycol 400 on a Celite 545 support. 3. By using 18O-enriched oxygen gas the abundance of 18O in the methanol formed from oxidation of methane was measured with a Perkin–Elmer 270 combined gas chromatograph/mass spectrometer. The results showed that the oxygen in methanol was derived exclusively from gaseous oxygen in both micro-organisms. 4. Control experiments using [18O]water in incubation mixtures confirmed that there was negligible incorporation of the oxygen atom from water into methanol.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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