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. 1970 Jul;118(3):497–503. doi: 10.1042/bj1180497

The mechanism of uptake of cobalt ions by Neurospora crassa

G Venkateswerlu 1, K Sivarama Sastry 1
PMCID: PMC1179218  PMID: 5472176

Abstract

Uptake of Co2+ by 3-day-old mycelia of Neurospora crassa involves cell-surface binding as well as transport into the intracellular space. The surface binding is rapid and accounts for 30–40% of the total Co2+ uptake. Transport of Co2+ occurs at a rate of 40μg/h per 100mg dry wt. Surface binding and overall uptake show different temperature dependence. Metabolic inhibitors such as azide, dinitrophenol and fluoride depress transport of Co2+. The overall uptake of Co2+ exhibits a high Km value and hence the concentration mechanism is one of low `affinity' for the metal. The uptake of Co2+ varies linearly with pH in the range pH3 to pH6. Mg2+ inhibits both surface binding and transport of Co2+. It is suggested that the system that transports Mg2+ is also involved in Co2+ uptake by N. crassa.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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