Abstract
The inhibition of N-acetyl-β-d-glucosaminase activity by 2-acetamido-2-deoxy-d-gluconolactone was examined. Separation of the (1→4)- and (1→5)-lactones was achieved by using paper chromatography or countercurrent distribution and identification was obtained by examination of the relative stability of the components of separated material. Quantitative measurement of the two lactones was by kinetic titration or by a colorimetric method based on their reaction with hydroxylamine. It was shown that only the (1→5)-lactone acted as an inhibitor of N-acetyl-β-d-glucosaminase.
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Selected References
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- BRODIE A. F., LIPMANN F. Identification of a gluconolactonase. J Biol Chem. 1955 Feb;212(2):677–685. [PubMed] [Google Scholar]
- CONCHIE J., FINDLAY J., LEVVY G. A. Alpha-mannosidase and other glycosidases in the tissues of the mouse and the rat, with special reference to the sex organs. Nature. 1956 Dec 29;178(4548):1469–1470. doi: 10.1038/1781469a0. [DOI] [PubMed] [Google Scholar]
- CONCHIE J., LEVVY G. A. Inhibition of glycosidases by aldonolactones of corresponding configuration. Biochem J. 1957 Feb;65(2):389–395. doi: 10.1042/bj0650389. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CORI O., LIPMANN F. The primary oxidation product of enzymatic glucose-6-phosphate oxidation. J Biol Chem. 1952 Jan;194(1):417–425. [PubMed] [Google Scholar]
- Conchie J., Hay A. J., Strachan I., Levvy G. A. Inhibition of glycosidases by aldonolactones of corresponding configuration: Preparation of (1-->5)-lactones by catalytic oxidation of pyranoses and study of their inhibitory properties. Biochem J. 1967 Mar;102(3):929–941. doi: 10.1042/bj1020929. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FINDLAY J., LEVVY G. A., MARSH C. A. Inhibition of glycosidases by aldonolactones of corresponding configuration. 2. Inhibitors of beta-N-acetylglucosaminidase. Biochem J. 1958 Jul;69(3):467–476. doi: 10.1042/bj0690467. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leaback D. H. On the inhibition of beta-N-acetyl-D-glucosaminidase by 2-acetamido-2-deoxy-D-glucono-(1-5)-lactone. Biochem Biophys Res Commun. 1968 Sep 30;32(6):1025–1030. doi: 10.1016/0006-291x(68)90132-0. [DOI] [PubMed] [Google Scholar]
- Levvy G. A., Hay A. J., Conchie J. Inhibition of glycosidases by aldonolactones of corresponding configuration. 4. Inhibitors of mannosidase and glucosidase. Biochem J. 1964 May;91(2):378–384. doi: 10.1042/bj0910378. [DOI] [PMC free article] [PubMed] [Google Scholar]