Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1970 Oct;119(4):699–706. doi: 10.1042/bj1190699

Characterization of messenger-like ribonucleic acid from Saccharomyces cerevisiae by the use of chromatography on methylated albumin–kieselguhr

Roger Johnson 1
PMCID: PMC1179456  PMID: 4321853

Abstract

Chromatography on methylated albumin–kieselguhr of RNA from Saccharomyces cerevisiae was used to separate stable RNA from a tenaciously bound DNA-like RNA fraction. The tenaciously bound RNA, which was eluted with a dilute solution of sodium dodecyl sulphate, was characterized as messenger-like RNA by its sedimentation behaviour, nucleotide composition, lack of methylated bases and labelling kinetics. Chromatography of purified ribosomal RNA indicated a minor contamination of the tenaciously bound fraction with ribosomal RNA. On the other hand, a large portion of pulse-labelled polyribosomal RNA from protoplasts of Saccharomyces cerevisiae was tenaciously bound to the columns. The `chase' of isotopic label from the messenger-like RNA was found to be retarded during inhibition of protein synthesis both by cycloheximide and by starvation for a carbon source.

Full text

PDF
699

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DAVIDSON J. N., SMELLIE R. M. S. Phosphorus compounds in the cell. II. The separation by ionophoresis on paper of the constituent nucleotides of ribonucleic acid. Biochem J. 1952 Dec;52(4):594–599. doi: 10.1042/bj0520594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DUELL E. A., INOUE S., UTTER M. F. ISOLATION AND PROPERTIES OF INTACT MITOCHONDRIA FROM SPHEROPLASTS OF YEAST. J Bacteriol. 1964 Dec;88:1762–1773. doi: 10.1128/jb.88.6.1762-1773.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ellem K. A., Rhode S. L., 3rd The use of guanidine thiocyanate to fractionate DNA-like RNA adsorbed on methylated bovine albumin-kieselguhr columns. Biochim Biophys Acta. 1969 Jan 21;174(1):117–123. doi: 10.1016/0005-2787(69)90234-2. [DOI] [PubMed] [Google Scholar]
  4. Ellem K. A. Some properties of mammalian DNA-like RNA isolated by chromatography on methylated bovine serum albumin-kieselguhr columns. J Mol Biol. 1966 Sep;20(2):283–305. doi: 10.1016/0022-2836(66)90065-9. [DOI] [PubMed] [Google Scholar]
  5. FAN D. P., HIGA A., LEVINTHAL C. MESSENGER RNA DECAY AND PROTECTION. J Mol Biol. 1964 Feb;8:210–222. doi: 10.1016/s0022-2836(64)80130-3. [DOI] [PubMed] [Google Scholar]
  6. FRAENKEL-CONRAT H., SINGER B., TSUGITA A. Purification of viral RNA by means of bentonite. Virology. 1961 May;14:54–58. doi: 10.1016/0042-6822(61)90131-3. [DOI] [PubMed] [Google Scholar]
  7. GILBERT W. Polypeptide synthesis in Escherichia coli. I. Ribosomes and the active complex. J Mol Biol. 1963 May;6:374–388. doi: 10.1016/s0022-2836(63)80050-9. [DOI] [PubMed] [Google Scholar]
  8. Hartlief R., Koningsberger V. V. Characterization of messenger RNA in protoplasts of Saccharomyces carlsbergensis. Biochim Biophys Acta. 1968 Sep 24;166(2):512–531. doi: 10.1016/0005-2787(68)90239-6. [DOI] [PubMed] [Google Scholar]
  9. Hartwell L. H., McLaughlin C. S. A mutant of yeast apparently defective in the initiation of protein synthesis. Proc Natl Acad Sci U S A. 1969 Feb;62(2):468–474. doi: 10.1073/pnas.62.2.468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hartwell L. H., McLaughlin C. S. Temperature-sensitive mutants of yeast exhibiting a rapid inhibition of protein synthesis. J Bacteriol. 1968 Nov;96(5):1664–1671. doi: 10.1128/jb.96.5.1664-1671.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hutchison H. T., Hartwell L. H. Macromolecule synthesis in yeast spheroplasts. J Bacteriol. 1967 Nov;94(5):1697–1705. doi: 10.1128/jb.94.5.1697-1705.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. KITAZUME Y., YCAS M. THE CALCULATED COMPOSITION OF NEWLY SYNTHESIZED YEAST RIBONUCLEIC ACID. Biochim Biophys Acta. 1963 Nov 22;76:391–400. [PubMed] [Google Scholar]
  13. Kunz W., Niessing J., Schnieders B., Sekeris C. E. Characterization of rapidly labelled rat liver ribonucleic acid showing high affinity for columns of methylated albumin on kieselguhr. Biochem J. 1970 Feb;116(4):563–567. doi: 10.1042/bj1160563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lingrel J. B. Studies on the rapidly labeled RNA of rabbit bone marrow cells. Biochim Biophys Acta. 1967 Jun 20;142(1):75–88. doi: 10.1016/0005-2787(67)90516-3. [DOI] [PubMed] [Google Scholar]
  15. MANDELL J. D., HERSHEY A. D. A fractionating column for analysis of nucleic acids. Anal Biochem. 1960 Jun;1:66–77. doi: 10.1016/0003-2697(60)90020-8. [DOI] [PubMed] [Google Scholar]
  16. NAKADA D., FAN D. P. PROTECTION OF BETA-GALACTOSIDASE MESSENGER RNA FROM DECAY DURING ANAEROBIOSIS IN ESCHERICHIA COLI. J Mol Biol. 1964 Feb;8:223–230. doi: 10.1016/s0022-2836(64)80131-5. [DOI] [PubMed] [Google Scholar]
  17. Retèl J., Planta R. J. Ribosomal precursor RNA in Saccharomyces carlsbergensis. Eur J Biochem. 1967 Dec;3(2):248–258. doi: 10.1111/j.1432-1033.1967.tb19524.x. [DOI] [PubMed] [Google Scholar]
  18. Retèl J., Planta R. J. The investigation of the ribosomal RNA sites in yeast DNA by the hybridization technique. Biochim Biophys Acta. 1968 Dec 17;169(2):416–429. doi: 10.1016/0005-2787(68)90050-6. [DOI] [PubMed] [Google Scholar]
  19. Roberts W. K., Quinilivan V. D. Purification and properties of nuclear and cytoplasmic "deoxyribonucleic acid like" ribonucleic acid from ehrlich ascites cells. Biochemistry. 1969 Jan;8(1):288–294. doi: 10.1021/bi00829a041. [DOI] [PubMed] [Google Scholar]
  20. Salser W., Janin J., Levinthal C. Measurement of the unstable RNA in exponentially growing cultures of Bacillus subtilis and Escherichia coli. J Mol Biol. 1968 Jan 28;31(2):237–266. doi: 10.1016/0022-2836(68)90442-7. [DOI] [PubMed] [Google Scholar]
  21. Schweizer E., MacKechnie C., Halvorson H. O. The redundancy of ribosomal and transfer RNA genes in Saccharomyces cerevisiae. J Mol Biol. 1969 Mar 14;40(2):261–277. doi: 10.1016/0022-2836(69)90474-4. [DOI] [PubMed] [Google Scholar]
  22. Taber R. L., Jr, Vincent W. S. The synthesis and processing of ribosomal RNA precursor molecules in yeast. Biochim Biophys Acta. 1969 Aug 20;186(2):317–325. doi: 10.1016/0005-2787(69)90009-4. [DOI] [PubMed] [Google Scholar]
  23. Wehr C. T., Parks L. W. Macromolecular synthesis in Saccharomyces cerevisiae in different growth media. J Bacteriol. 1969 May;98(2):458–466. doi: 10.1128/jb.98.2.458-466.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wintersberger E. A distinct class of ribosomal RNA components in yeast mitochondria as revealed by gradient centrifugation and by DNA-RNA-hybridization. Hoppe Seylers Z Physiol Chem. 1967 Dec;348(12):1701–1704. [PubMed] [Google Scholar]
  25. Yoshikawa-Fukada M., Fukada T., Kawade Y. Characterization of rapidly labeled ribonucleic acid of animal cells in culture. Biochim Biophys Acta. 1965 Jul 15;103(3):383–398. doi: 10.1016/0005-2787(65)90132-2. [DOI] [PubMed] [Google Scholar]
  26. de Kloet S. R. Ribonucleic acid synthesis in yeast. The effect of cycloheximide on the synthesis of ribonucleic acid in Saccharomyces carlsbergensis. Biochem J. 1966 Jun;99(3):566–581. doi: 10.1042/bj0990566. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES