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. 1970 Nov;120(2):345–351. doi: 10.1042/bj1200345

Tricarboxylic acid-cycle metabolism in brain. Effect of fluoroacetate and fluorocitrate on the labelling of glutamate aspartate, glutamine and γ-amino butyrate

D D Clarke 1, W J Nicklas 1,*, S Berl 2
PMCID: PMC1179604  PMID: 5493856

Abstract

1. The effect of fluoroacetate and fluorocitrate on the compartmentation of the glutamate–glutamine system was studied in brain slices with l-[U-14C]glutamate, l-[U-14C]aspartate, [1-14C]acetate and γ-amino[1-14C]butyrate as precursors and in homogenates of brain tissue with [1-14C]acetate. The effect of fluoroacetate was also studied in vivo in mouse brain with [1-14C]acetate as precursor. 2. Fluoroacetate and fluorocitrate inhibit the labelling of glutamine from all precursors but affect the labelling of glutamate to a much lesser extent. This effect is not due to inhibition of glutamine synthetase. It is interpreted as being due to selective inhibition of the metabolism of a small pool of glutamate that preferentially labels glutamine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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