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. 1970 Nov;120(2):373–379. doi: 10.1042/bj1200373

Comparison of immunoadsorbents prepared by coupling sperm-whale myoglobin to a variety of insoluble polymers

R J Boegman 1,*, M J Crumpton 1
PMCID: PMC1179607  PMID: 5493858

Abstract

Immunoadsorbents that were potentially suitable for the fractionation of antibodies to myoglobin were prepared by coupling myoglobin to aminoethylcellulose, bromoacetylcellulose, CM-cellulose, an ethylene–maleic anhydride co-polymer and Sepharose 4B. A comparison of the properties of the preparations indicated that Sepharose–myoglobin was superior, as an immunoadsorbent, to the other conjugates. Elution of adsorbed antibodies with m-propionic acid, pH2.5, gave yields of precipitable antibody higher than those achieved with other dissociating solvents. Propionic acid eluted 60–100% of the antibody adsorbed by Sepharose–myoglobin, and 60–90% of the eluted protein was precipitable antibody.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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