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. 1970 Dec;120(3):539–547. doi: 10.1042/bj1200539

Conformation of nucleic acids and the analysis of the hypochromic effect

R A Cox 1
PMCID: PMC1179635  PMID: 5499966

Abstract

The spectra of two double-helical RNA species isolated from virus-like particles found in the mycelium of Penicillium chrysogenum were measured at about 25°C, and at 95°C after denaturation to a single-stranded form, and compared with the spectrum of the equivalent mixture of nucleotides. For both species the difference spectrum (ε95°C–ε25°C) when increased by 33% was very similar to the difference spectrum (εnucleotides–ε25°C). In contrast it was found for rat liver DNA that there was no simple relation between (ε95°C–ε25°C) and (εnucleotides–ε25°C). These observations were accounted for on the basis of the difference spectra for rA·rU, rG·rC, dA·dT and dG·dC base-pairs derived from model polyribonucleotides and polydeoxyribonucleotides. The difference spectra (ε95°C–ε25°C) found for rRNA and those calculated from a combination of the difference spectra for rA·rU and rG·rC base-pairs were in good agreement.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boedtker H., Kelling D. G. The ordered structure of 5S RNA. Biochem Biophys Res Commun. 1967 Dec 15;29(5):758–766. doi: 10.1016/0006-291x(67)90283-5. [DOI] [PubMed] [Google Scholar]
  2. Cotter R. I., Gratzer W. B. Conformation of ribosomal RNA of E. coli: an infrared analysis. Nature. 1969 Jan 11;221(5176):154–156. doi: 10.1038/221154a0. [DOI] [PubMed] [Google Scholar]
  3. Cox R. A. A possible method for characterizing the secondary structure of ribonucleic acids. Biochem J. 1966 Jul;100(1):146–168. doi: 10.1042/bj1000146. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cox R. A. A spectrophotometric study of the secondary structure of ribonucleic acid isolated from the smaller and larger ribosomal subparticles of rabbit reticulocytes. Biochem J. 1970 Mar;117(1):101–118. doi: 10.1042/bj1170101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cox R. A., Kanagalingam K., Sutherland K. S. Double-helical character of ribonucleic acid from virus-like particles found in Penicillium chrysogenum. Biochem J. 1970 Dec;120(3):549–558. doi: 10.1042/bj1200549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cox R. A. The secondary structure of ribosomal ribonucleic acid in solution. Biochem J. 1966 Mar;98(3):841–857. doi: 10.1042/bj0980841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FELSENFELD G., SANDEEN G. The dispersion of the hyperchromic effect in thermally induced transitions of nucleic acids. J Mol Biol. 1962 Dec;5:587–610. doi: 10.1016/s0022-2836(62)80088-6. [DOI] [PubMed] [Google Scholar]
  8. Felsenfeld G., Hirschman S. Z. A neighbor-interaction analysis of the hypochromism and spectra of DNA. J Mol Biol. 1965 Sep;13(2):407–427. doi: 10.1016/s0022-2836(65)80106-1. [DOI] [PubMed] [Google Scholar]
  9. Guschlbauer W. Helical regions in transfer ribonucleic acid. Biophysik. 1966;3(2):156–164. doi: 10.1007/BF01191609. [DOI] [PubMed] [Google Scholar]
  10. MAHLER H. R., KLINE B., MEHROTRA B. D. SOME OBSERVATIONS ON THE HYPOCHROMISM OF DNA. J Mol Biol. 1964 Sep;9:801–811. doi: 10.1016/s0022-2836(64)80186-8. [DOI] [PubMed] [Google Scholar]
  11. Richards E. G., Simpkins H. A comparison of some properties of alternating poly(A-U) and the two stranded complex of poly A and poly U. Eur J Biochem. 1968 Oct 17;6(1):93–97. doi: 10.1111/j.1432-1033.1968.tb00424.x. [DOI] [PubMed] [Google Scholar]
  12. Zmudzka B., Bollum F. J., Shugar D. Polydeoxyribouridylic acid and its complexes with polyribo- and deoxyriboadenylic acids. J Mol Biol. 1969 Nov 28;46(1):169–183. doi: 10.1016/0022-2836(69)90064-3. [DOI] [PubMed] [Google Scholar]

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