Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1970 Dec;120(4):787–795. doi: 10.1042/bj1200787

Chemical properties of guinea-pig immunoglobulins γ2G and γM

R G Q Leslie 1,*, S Cohen 1
PMCID: PMC1179672  PMID: 5495151

Abstract

The isolation of guinea-pig immunoglobulins γ1G, γ2G and γM are described and methods for separating the polypeptide chains of each examined. The molecular weights, extinction coefficients and carbohydrate and amino acid compositions of the immunoglobulins and their constituent chains have been analysed. The findings provide a basis for further studies attempting to relate structural differences to distinct biological properties of guinea-pig immunoglobulins.

Full text

PDF
787

Images in this article

789Fig. 3.
on p.789
789Fig. 4.
on p.789

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMINOFF D. Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochem J. 1961 Nov;81:384–392. doi: 10.1042/bj0810384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BENACERRAF B., OVARY Z., BLOCH K. J., FRANKLIN E. C. Properties of guinea pig 7S antibodies. I. Electrophoretic separation of two types of guinea pig 7S antibodies. J Exp Med. 1963 Jun 1;117:937–949. doi: 10.1084/jem.117.6.937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BLOCH K. J., KOURILSKY F. M., OVARY Z., BENACERRAF B. PROPERTIES OF GUINEA PIG 7S ANTIBODIES. IV. ANTIBODY RESPONSE TO E. COLI BACTERIA. Proc Soc Exp Biol Med. 1963 Oct;114:52–56. doi: 10.3181/00379727-114-28583. [DOI] [PubMed] [Google Scholar]
  4. BLOCH K. J., KOURILSKY F. M., OVARY Z., BENACERRAF B. Properties of guinea pig 7S antibodies. III. Identification of antibodies involved in complement fixation and hemolysis. J Exp Med. 1963 Jun 1;117:965–981. doi: 10.1084/jem.117.6.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Beale D., Feinstein A. Evidence for C-terminal intra-subunit disulphide bridges between immunoglobulin-M heavy chains. FEBS Lett. 1970 Apr 2;7(2):175–176. doi: 10.1016/0014-5793(70)80149-1. [DOI] [PubMed] [Google Scholar]
  6. Berken A., Benacerraf B. Properties of antibodies cytophilic for macrophages. J Exp Med. 1966 Jan 1;123(1):119–144. doi: 10.1084/jem.123.1.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Binaghi R. A. Production of 7 S immunoglobulins in immunized guinea pigs. J Immunol. 1966 Aug;97(2):159–164. [PubMed] [Google Scholar]
  8. CRUMPTON M. J., WILKINSON J. M. AMINO ACID COMPOSITIONS OF HUMAN AND RABBIT GAMMA-GLOBULINS AND OF THE FRAGMENTS PRODUCED BY REDUCTION. Biochem J. 1963 Aug;88:228–234. doi: 10.1042/bj0880228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cebra J. J., Small P. A., Jr Polypeptide chain structure of rabbit immunoglobulins. 3. Secretory gamma-A-immunoglobulin from colostrum. Biochemistry. 1967 Feb;6(2):503–512. doi: 10.1021/bi00854a019. [DOI] [PubMed] [Google Scholar]
  10. Del Guercio P., Tolone G., Andrade F. B., Biozzi G., Binaghi R. A. Opsonic, cytophilic and agglutinating activity of guinea-pig gamma-2 and gamma-M anti-Salmonella antibodies. Immunology. 1969 Mar;16(3):361–371. [PMC free article] [PubMed] [Google Scholar]
  11. FLEISCHMAN J. B., PORTER R. R., PRESS E. M. THE ARRANGEMENT OF THE PEPTIDE CHAINS IN GAMMA-GLOBULIN. Biochem J. 1963 Aug;88:220–228. doi: 10.1042/bj0880220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. JOHANSEN P. G., MARSHALL R. D., NEUBERGER A. Carbohydrates in protein. 2. The hexose, hexosamine, acetyl and amide-nitrogen content of hen's-egg albumin. Biochem J. 1960 Nov;77:239–247. doi: 10.1042/bj0770239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lamm M. E. Further comparison of guinea pig gamma-1-and gamma-2-immunoglobulins. Amino acid compositions of the polypeptide chains and papain fragments. Immunochemistry. 1969 Mar;6(2):235–240. doi: 10.1016/0019-2791(69)90160-8. [DOI] [PubMed] [Google Scholar]
  14. Lamm M. E., Small P. A., Jr Polypeptide chain structure of rabbit immunoglobulins. II. gamma-M-immunoglobulin. Biochemistry. 1966 Jan;5(1):267–276. doi: 10.1021/bi00865a035. [DOI] [PubMed] [Google Scholar]
  15. Linscott W. D., Hansen S. S. Non-complement-fixing IgM antibodies in the guinea pig. J Immunol. 1969 Sep;103(3):423–428. [PubMed] [Google Scholar]
  16. MELAMED M. D., GREEN N. M. AVIDIN. 2. PURIFICATION AND COMPOSITION. Biochem J. 1963 Dec;89:591–599. doi: 10.1042/bj0890591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. MILLER F., METZGER H. CHARACTERIZATION OF A HUMAN MACROGLOBULIN. I. THE MOLECULAR WEIGHT OF ITS SUBUNIT. J Biol Chem. 1965 Aug;240:3325–3333. [PubMed] [Google Scholar]
  18. Milstein C. Chemical structure of light chains. Proc R Soc Lond B Biol Sci. 1966 Nov 22;166(1003):138–149. doi: 10.1098/rspb.1966.0089. [DOI] [PubMed] [Google Scholar]
  19. Nussenzweig V., Lamm M. E., Benacerraf B. Presence of two types of L polypeptide chains in guinea pig 7S immunoglobulins. J Exp Med. 1966 Nov 1;124(5):787–803. doi: 10.1084/jem.124.5.787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. O'Donnell I. J., Frangione B., Porter R. R. The disulphide bonds of the heavy chain of rabbit immunoglobulin G. Biochem J. 1970 Jan;116(2):261–268. doi: 10.1042/bj1160261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. OETTGEN H. F., BINAGHI R. A., BENACERRAF B. HEXOSE CONTENT OF GUINEA PIG GAMMA-1 AND GAMMA-2 IMMUNOGLOBULINS. Proc Soc Exp Biol Med. 1965 Feb;118:336–342. [PubMed] [Google Scholar]
  22. OVARY Z., BENACERRAF B., BLOCH K. J. Properties of guinea pig 7S antibodies. II. Identification of antibodies involved in passive cutaneous and systemic anaphylaxis. J Exp Med. 1963 Jun 1;117:951–964. doi: 10.1084/jem.117.6.951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Osler A. G., Oliveira B., Shin H. S., Sandberg A. L. The fixation of guinea pig complement by gamma 1 and gamma 2 immunoglobulins. J Immunol. 1969 Jan;102(1):269–271. [PubMed] [Google Scholar]
  24. PALMER J. L., NISONOFF A. DISSOCIATION OF RABBIT GAMMA-GLOBULIN INTO HALF-MOLECULES AFTER REDUCTION OF ONE LABILE DISULFIDE BOND. Biochemistry. 1964 Jun;3:863–869. doi: 10.1021/bi00894a024. [DOI] [PubMed] [Google Scholar]
  25. Piggot P. J., Press E. M. Cyanogen bromide cleavage and partial sequence of the heavy chain of a pathological immunoglobulin G. Biochem J. 1967 Aug;104(2):616–626. doi: 10.1042/bj1040616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Robbins J. B., Haimovich J., Sela M. Purification of antibodies with immunoadsorbents prepared using bromoacetyl cellulose. Immunochemistry. 1967 Jan;4(1):11–22. doi: 10.1016/0019-2791(67)90192-9. [DOI] [PubMed] [Google Scholar]
  27. SCHEIDEGGER J. J. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed] [Google Scholar]
  28. Small P. A., Jr, Lamm M. E. Polypeptide chain structure of rabbit immunoglobulins. I. gamma-G-immunoglobulin. Biochemistry. 1966 Jan;5(1):259–267. doi: 10.1021/bi00865a034. [DOI] [PubMed] [Google Scholar]
  29. Strejan G., Campbell D. H. Hypersensitivity to Ascaris antigens. II. The skin-sensitizing properties of 7 S-gamma-2 antibody from sensitized guinea pigs as tested in guinea pigs. J Immunol. 1967 Aug;99(2):347–356. [PubMed] [Google Scholar]
  30. Strejan G., Campbell D. H. Skin-sensitizing properties of guinea pig antibodies to keyhole limpet hemocyanin. J Immunol. 1968 Jun;100(6):1245–1254. [PubMed] [Google Scholar]
  31. WHITE R. G., JENKINS G. C., WILKINSON P. C. The production of skin-sensitizing antibody in the guinea-pig. Int Arch Allergy Appl Immunol. 1963;22:156–165. doi: 10.1159/000229362. [DOI] [PubMed] [Google Scholar]
  32. YAGI Y., MAIER P., PRESSMAN D. Immunoelectrophoretic identification of guinea pig anti-insulin antibodies. J Immunol. 1962 Nov;89:736–744. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES