Table 7.
Serum biochemical, antioxidant, and immunological parameters in turbot before or after V. harveyi challenge.
| Item | Control | SM | FPH |
|---|---|---|---|
| Before V. harveyi challenge | |||
| Alb (g/L) | 7.75 ± 0.60 | 8.80 ± 1.03 | 9.97 ± 0.98 |
| TP (g/L) | 30.23 ± 1.62 | 26.22 ± 1.61 | 30.34 ± 1.12 |
| T-SOD (U/mL) | 30.71 ± 0.79 | 32.40 ± 0.04 | 32.01 ± 1.27 |
| CAT (U/mL) | 29.23 ± 2.02a | 26.19 ± 1.43a | 13.45 ± 1.52b |
| GSH-PX (U/mL) | 56.92 ± 6.99 | 52.89 ± 5.09 | 56.89 ± 5.96 |
| MDA (nmol/mL) | 13.22 ± 0.83 | 13.90 ± 0.41 | 13.28 ± 0.24 |
| MPO (U/L) | 303.45 ± 0.16b | 338.36 ± 6.19a | 237.03 ± 8.94c |
| GOT (U/L) | 16.26 ± 2.15 | 24.12 ± 0.94 | 17.63 ± 2.33 |
| GPT (U/L) | 9.59 ± 1.02 | 11.62 ± 0.62 | 10.29 ± 1.06 |
| LZM (μg/mL) | 7.41 ± 0.26 | 7.08 ± 0.24 | 7.94 ± 0.18 |
| T-NOS (U/mL) | 26.38 ± 1.96 | 27.99 ± 1.90 | 31.45 ± 1.12 |
| IgM (μg/ml) | 309.27 ± 17.12b | 305.88 ± 33.24b | 472.75 ± 46.12a |
| C3 (μg/ml) | 888.02 ± 49.84 | 766.08 ± 106.48 | 1011.82 ± 159.79 |
| After V. harveyi challenge | |||
| Alb (g/L) | 7.77 ± 0.35a | 3.59 ± 0.63b | 4.85 ± 0.69ab |
| TP (g/L) | 19.17 ± 0.57b | 12.51 ± 0.84c | 24.57 ± 0.40a |
| T-SOD (U/mL) | 24.55 ± 0.63 | 26.03 ± 1.08 | 23.64 ± 1.16 |
| CAT (U/mL) | 27.42 ± 2.47 | 17.17 ± 0.59 | 20.02 ± 2.60 |
| GSH-PX (U/ml) | 18.29 ± 1.89 | 22.41 ± 2.81 | 15.75 ± 1.22 |
| MDA (nmol/mL) | 9.88 ± 0.27a | 7.96 ± 0.35b | 8.59 ± 0.35ab |
| MPO (U/L) | 358.68 ± 8.51 | 375.67 ± 12.42 | 347.97 ± 15.64 |
| GOT (U/L) | 21.98 ± 0.64 | 20.73 ± 0.95 | 19.66 ± 2.21 |
| GPT (U/L) | 16.51 ± 0.67 | 14.45 ± 0.41 | 16.29 ± 0.73 |
| LZM (μg/mL) | 7.43 ± 0.10b | 8.18 ± 0.02a | 8.14 ± 0.05a |
| T-NOS (U/mL) | 17.52 ± 1.21 | 20.65 ± 0.50 | 21.47 ± 0.91 |
| IgM (μg/mL) | 272.11 ± 17.07 | 297.39 ± 17.88 | 321.85 ± 29.06 |
| C3 (μg/mL) | 857.06 ± 88.34 | 927.92 ± 58.80 | 708.23 ± 45.96 |
Note: Values are means and standard error of three replicate tanks. Values in the same row followed by different superscript letters are significantly different (p < 0.05) according to Tukeys' multiple comparison test.
Abbreviations: ALB, albumin; C3, complement 3; CAT, catalase; FPH, fish protein hydrolysate; GOP, glutamic oxalic aminotransferase; GPT, glutamic pyruvic aminotransferase; GSH-PX, glutathione peroxidase; IgM, immunoglobulin M; LZM, lysozyme; MDA, malondialdehyde; MPO, myeloperoxidase; SM, soybean meal; T-NOS, total nitric oxide synthase; TP, total protein; T-SOD, total superoxide dismutase.