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. 1986 May;374:401–411. doi: 10.1113/jphysiol.1986.sp016087

Beta-adrenergic inhibition of bovine mesenteric lymphatics.

J M Allen, H L Iggulden, N G McHale
PMCID: PMC1182728  PMID: 3746697

Abstract

The beta-action of catecholamines on lymphatic smooth muscle was studied by observing the effect of isoprenaline on electrical and mechanical activity in the double sucrose-gap. Action potentials and phasic contractions evoked by depolarizing pulses were abolished within 2 min of drug addition. Isoprenaline hyperpolarized the membrane and increased membrane conductance. Tetraethylammonium (10 mM) did not itself affect membrane resistance but reduced the hyperpolarization and the increase in conductance caused by isoprenaline. Removal of K+ from the external solution reduced membrane conductance and increased the hyperpolarization due to isoprenaline. When the NaCl content of Krebs solution was replaced with LiCl or choline chloride, isoprenaline no longer blocked action potential firing and its effects on phasic contractions and membrane conductance were reduced. In contrast, ouabain (10(-5) M) did not block the effect of isoprenaline on membrane potential and membrane conductance. These results suggest that beta-adrenergic inhibition of lymphatic smooth muscle involves an increase in an outward K+ current, though an additional metabolic effect cannot be ruled out.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARMSTRONG C. M., BINSTOCK L. ANOMALOUS RECTIFICATION IN THE SQUID GIANT AXON INJECTED WITH TETRAETHYLAMMONIUM CHLORIDE. J Gen Physiol. 1965 May;48:859–872. doi: 10.1085/jgp.48.5.859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aberg G., Adler G. The effect of age on -adrenoceptor activity in tracheal smooth muscle. Br J Pharmacol. 1973 Jan;47(1):181–182. doi: 10.1111/j.1476-5381.1973.tb08173.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Allen J. M., McHale N. G., Rooney B. M. Effect of norepinephrine on contractility of isolated mesenteric lymphatics. Am J Physiol. 1983 Apr;244(4):H479–H486. doi: 10.1152/ajpheart.1983.244.4.H479. [DOI] [PubMed] [Google Scholar]
  4. Brading A. F. Calcium-induced increase in membrane permeability in the guinea-pig taenia coli: evidence for involvement of a sodium-calcium exchange mechanism. J Physiol. 1978 Feb;275:65–84. doi: 10.1113/jphysiol.1978.sp012178. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bülbring E., Tomita T. Increase of membrane conductance by adrenaline in the smooth muscle of guinea-pig taenia coli. Proc R Soc Lond B Biol Sci. 1969 Mar 11;172(1027):89–102. doi: 10.1098/rspb.1969.0013. [DOI] [PubMed] [Google Scholar]
  6. Bülbring E., den Hertog A. The action of isoprenaline on the smooth muscle of the guinea-pig taenia coli. J Physiol. 1980 Jul;304:277–296. doi: 10.1113/jphysiol.1980.sp013324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Casteels R., Van Breemen C. Active and passive Ca2+ fluxes across cell membranes of the guinea-pig taenia coli. Pflugers Arch. 1975 Sep 9;359(3):197–207. doi: 10.1007/BF00587379. [DOI] [PubMed] [Google Scholar]
  8. Diamond J., Marshall J. M. Smooth muscle relaxants: dissociation between resting membrane potential and resting tension in rat myometrium. J Pharmacol Exp Ther. 1969 Jul;168(1):13–20. [PubMed] [Google Scholar]
  9. Fleisch J. H., Maling H. M., Brodie B. B. Beta-receptor activity in aorta; variations with age and species. Circ Res. 1970 Feb;26(2):151–162. doi: 10.1161/01.res.26.2.151. [DOI] [PubMed] [Google Scholar]
  10. Johansson B., Johsson O., Axelsson J., Wahlström B. Electrical and mechanical characteristics of vascular smooth muscle response to norepinephrine and isoproterenol. Circ Res. 1967 Nov;21(5):619–633. doi: 10.1161/01.res.21.5.619. [DOI] [PubMed] [Google Scholar]
  11. Kirkpatrick C. T., McHale N. G. Electrical and mechanical activity of isolated lymphatic vessels [proceedings]. J Physiol. 1977 Oct;272(1):33P–34P. [PubMed] [Google Scholar]
  12. Kroeger E. A., Marshall J. M. Beta-adrenergic effects on rat myometrium: mechanisms of membrane hyperpolarization. Am J Physiol. 1973 Dec;225(6):1339–1345. doi: 10.1152/ajplegacy.1973.225.6.1339. [DOI] [PubMed] [Google Scholar]
  13. Levin R. M., Wein A. J. Distribution and function of adrenergic receptors in the urinary bladder. Mol Pharmacol. 1979 Sep;16(2):441–448. [PubMed] [Google Scholar]
  14. Ljung B., Isaksson O., Johansson B. Levels of cyclic AMP and electrical events during inhibition of contractile activity in vascular smooth muscle. Acta Physiol Scand. 1975 Jun;94(2):154–166. doi: 10.1111/j.1748-1716.1975.tb05875.x. [DOI] [PubMed] [Google Scholar]
  15. Magaribuchi T., Kuriyama H. Effects of noradrenaline and isoprenaline on the electrical and mechanical activities of guinea pig depolarized taenia coli. Jpn J Physiol. 1972 Jun;22(3):253–270. doi: 10.2170/jjphysiol.22.253. [DOI] [PubMed] [Google Scholar]
  16. Mawhinney H. J., Roddie I. C. Spontaneous activity in isolated bovine mesenteric lymphatics. J Physiol. 1973 Mar;229(2):339–348. doi: 10.1113/jphysiol.1973.sp010141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. McHale N. G., Roddie I. C. The effects of catecholamines on pumping activity in isolated bovine mesenteric lymphatics. J Physiol. 1983 May;338:527–536. doi: 10.1113/jphysiol.1983.sp014687. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. McHale N. G., Roddie I. C., Thornbury K. D. Nervous modulation of spontaneous contractions in bovine mesenteric lymphatics. J Physiol. 1980 Dec;309:461–472. doi: 10.1113/jphysiol.1980.sp013520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Reuter H., Blaustein M. P., Haeusler G. Na-Ca exchange and tension development in arterial smooth muscle. Philos Trans R Soc Lond B Biol Sci. 1973 Mar 15;265(867):87–94. doi: 10.1098/rstb.1973.0011. [DOI] [PubMed] [Google Scholar]
  20. Russell J. A., Zimmerman K., Middendorf W. F. Evidence for alpha-adrenergic innervation of the isolated canine thoracic duct. J Appl Physiol Respir Environ Exerc Physiol. 1980 Dec;49(6):1010–1015. doi: 10.1152/jappl.1980.49.6.1010. [DOI] [PubMed] [Google Scholar]
  21. Scheid C. R., Fay F. S. Beta-adrenergic stimulation of 42K influx in isolated smooth muscle cells. Am J Physiol. 1984 May;246(5 Pt 1):C415–C421. doi: 10.1152/ajpcell.1984.246.5.C415. [DOI] [PubMed] [Google Scholar]
  22. Schild H. O. The action of isoprenaline in the depolarized rat uterus. Br J Pharmacol Chemother. 1967 Nov;31(3):578–592. doi: 10.1111/j.1476-5381.1967.tb00422.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Walsh J. V., Jr, Singer J. J. Voltage clamp of single freshly dissociated smooth muscle cells: current-voltage relationships for three currents. Pflugers Arch. 1981 May;390(2):207–210. doi: 10.1007/BF00590209. [DOI] [PubMed] [Google Scholar]

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