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. 1986 Sep;378:335–345. doi: 10.1113/jphysiol.1986.sp016222

Electrogenic anion secretion in cultured rat epididymal epithelium.

A W Cuthbert, P Y Wong
PMCID: PMC1182867  PMID: 3467065

Abstract

Primary monolayer cultures enriched with principal cells from rat cauda epididymis were grown on pervious supports until confluence was reached. The epithelia so formed were used for short-circuit current recording. Epididymal monolayers had a transepithelial potential of 0.2 mV (apical side negative), a basal short-circuit current of 2 microA/cm2 and a transepithelial resistance of 60-90 omega cm2 when bathed on both sides with Krebs-Henseleit solution at 37 degrees C and gassed with 95% O2-5% CO2. 8-bromo-adenosine 3',5'-phosphate (1 mM) and forskolin (10 microM) caused an inward-flowing current in short-circuited monolayers. The current was partially sensitive to acetazolamide and to frusemide, suggesting that anion secretion was responsible for the responses. In the absence of chloride in the bathing fluid the response to forskolin was sensitive to acetazolamide but not to frusemide. The peptide lysylbradykinin (LBK) and prostaglandin E2 (PGE2) also produced inward-flowing currents which again appeared to be due to anion secretion. The actions of PGE2 were seen only when this agent was added to the basolateral side of the tissue, while LBK had effects from both sides. The responses to kinin, but not to PGE2, were inhibited by cyclo-oxygenase inhibitors such as piroxicam and indomethacin. It seems the kinin effects are dependent, at least in part, upon eicosanoid formation. Responses to kinin and PGE2 were severely attenuated in chloride-free bathing solution. Readdition of chloride-containing solution restored the responses to both agents. Assuming electrogenic ion secretion in the epididymis is accompanied by osmotic fluid flow it is evident that the transit time of sperm in the cauda epididymis will be reduced. Possible consequences for the maturation of spermatozoa are discussed.

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Selected References

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  1. BHOOLA K. D., MAY MAY YI R., MORLEY J., SCHACHTER M. Release of kinin by an enzyme in the accessory sex glands of the guinea-pig. J Physiol. 1962 Sep;163:269–280. doi: 10.1113/jphysiol.1962.sp006973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cuthbert A. W., George A. M., MacVinish L. Kinin effects on electrogenic ion transport in primary cultures of pig renal papillary collecting tubule cells. Am J Physiol. 1985 Sep;249(3 Pt 2):F439–F447. doi: 10.1152/ajprenal.1985.249.3.F439. [DOI] [PubMed] [Google Scholar]
  3. Cuthbert A. W., Halushka P. V., Kessel D., Margolius H. S., Wise W. C. Kinin effects on chloride secretion do not require eicosanoid synthesis. Br J Pharmacol. 1984 Oct;83(2):549–554. doi: 10.1111/j.1476-5381.1984.tb16519.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cuthbert A. W., Halushka P. V., Margolius H. S., Spayne J. A. Mediators of the secretory response to kinins. Br J Pharmacol. 1984 Jul;82(3):597–607. doi: 10.1111/j.1476-5381.1984.tb10798.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cuthbert A. W., Kirkland S. C., MacVinish L. J. Kinin effects on ion transport in monolayers of HCA-7 cells, a line from a human colonic adenocarcinoma. Br J Pharmacol. 1985 Sep;86(1):3–5. doi: 10.1111/j.1476-5381.1985.tb09428.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Geiger R., Clausnitzer B. Isolation of an enzymatically active tissue kallikrein from human seminal plasma by immunoaffinity chromatography. Hoppe Seylers Z Physiol Chem. 1981 Sep;362(9):1279–1283. [PubMed] [Google Scholar]
  7. Grasl M., Turnheim K. Stimulation of electrolyte secretion in rabbit colon by adenosine. J Physiol. 1984 Jan;346:93–110. doi: 10.1113/jphysiol.1984.sp015009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hib J., Ponzio R. O., Vilar O. Effect of chronic treatment with prostaglandin E2 on male rat fertility. Andrologia. 1979 Jul-Aug;11(4):326–330. doi: 10.1111/j.1439-0272.1979.tb02215.x. [DOI] [PubMed] [Google Scholar]
  9. Kierszenbaum A. L., Lea O., Petrusz P., French F. S., Tres L. L. Isolation, culture, and immunocytochemical characterization of epididymal epithelial cells from pubertal and adult rats. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1675–1679. doi: 10.1073/pnas.78.3.1675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Levine N., Marsh D. J. Micropuncture studies of the electrochemical aspects of fluid and electrolyte transport in individual seminiferous tubules, the epididymis and the vas deferens in rats. J Physiol. 1971 Mar;213(3):557–570. doi: 10.1113/jphysiol.1971.sp009400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Orgebin-Crist M. C., Djiane J. Properties of a prolactin receptor from the rabbit epididymis. Biol Reprod. 1979 Aug;21(1):135–139. doi: 10.1095/biolreprod21.1.135. [DOI] [PubMed] [Google Scholar]
  12. Robb G. W., Amann R. P., Killian G. J. Daily sperm production and epididymal sperm reserves of pubertal and adult rats. J Reprod Fertil. 1978 Sep;54(1):103–107. doi: 10.1530/jrf.0.0540103. [DOI] [PubMed] [Google Scholar]
  13. Seamon K. B., Padgett W., Daly J. W. Forskolin: unique diterpene activator of adenylate cyclase in membranes and in intact cells. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3363–3367. doi: 10.1073/pnas.78.6.3363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sharpe R. M., Fraser H. M., Cooper I., Rommerts F. F. The secretion, measurement, and function of a testicular LHRH-like factor. Ann N Y Acad Sci. 1982;383:272–294. doi: 10.1111/j.1749-6632.1982.tb23173.x. [DOI] [PubMed] [Google Scholar]
  15. Turner T. T. On the epididymis and its function. Invest Urol. 1979 Mar;16(5):311–321. [PubMed] [Google Scholar]
  16. White M. G., Huang Y. S., Tres L. L., Kierszenbaum A. L. Structural and functional aspects of cultured epididymal epithelial cells isolated from pubertal rats. J Reprod Fertil. 1982 Nov;66(2):475–484. doi: 10.1530/jrf.0.0660475. [DOI] [PubMed] [Google Scholar]
  17. Wong P. Y., Au C. L., Ngai H. K. The isolated duct of the rat cauda epididymidis as a model for isosmotic transport studies. Jpn J Physiol. 1980;30(1):1–15. doi: 10.2170/jjphysiol.30.1. [DOI] [PubMed] [Google Scholar]
  18. Wong P. Y., Yeung C. H. Absorptive and secretory functions of the perfused rat cauda epididymidis. J Physiol. 1978 Feb;275:13–26. doi: 10.1113/jphysiol.1978.sp012174. [DOI] [PMC free article] [PubMed] [Google Scholar]

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