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. 1977 Oct 1;167(1):281–284. doi: 10.1042/bj1670281

Release of alkaline phosphatase from membranes by a phosphatidylinositol-specific phospholipase C.

M G Low, J B Finean
PMCID: PMC1183647  PMID: 588258

Abstract

Purified phosphatidylinositol-specific phospholipase C from Staphylococcus aureus released a substantial proportion of the total alkaline phosphatase activity from a wide range of tissues from several mammalian species. Co-purification of the phospholipase C and alkaline phosphatase-releasing activities and the inhibition of both these activities by iso-osmotic salt solutions suggested that the releasing effect was unlikely to be due to a contaminant.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allan D., Michell R. H. Phosphatidylinositol cleavage catalysed by the soluble fraction from lymphocytes. Activity at pH5.5 and pH7.0. Biochem J. 1974 Sep;142(3):591–597. doi: 10.1042/bj1420591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ikezawa H., Yamanegi M., Taguchi R., Miyashita T., Ohyabu T. Studies on phosphatidylinositol phosphodiesterase (phospholipase C type) of Bacillus cereus. I. purification, properties and phosphatase-releasing activity. Biochim Biophys Acta. 1976 Nov 19;450(2):154–164. [PubMed] [Google Scholar]
  3. Little C., Aurebekk B., Otnaess A. B. Purification by affinity chromatography of phospholipase C from Bacillus cereus. FEBS Lett. 1975 Apr 1;52(2):175–179. doi: 10.1016/0014-5793(75)80800-3. [DOI] [PubMed] [Google Scholar]
  4. Louvard D., Maroux S., Baratti J., Desnuelle P. On the distribution of enterokinase in porcine intestine and on its subcellular localization. Biochim Biophys Acta. 1973 May 5;309(1):127–137. doi: 10.1016/0005-2744(73)90324-0. [DOI] [PubMed] [Google Scholar]
  5. Low M. G., Finean J. B. Modification of erythrocyte membranes by a purified phosphatidylinositol-specific phospholipase C (Staphylococcus aureus). Biochem J. 1977 Feb 15;162(2):235–240. doi: 10.1042/bj1620235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Low M. G., Finean J. B. The action of phosphatidylinositol-specific phospholipases C on membranes. Biochem J. 1976 Jan 15;154(1):203–208. doi: 10.1042/bj1540203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nordström C. Enzymic release of enteropeptidase from isolated rat duodenal brush borders. Biochim Biophys Acta. 1972 Jun 16;268(3):711–718. doi: 10.1016/0005-2744(72)90275-6. [DOI] [PubMed] [Google Scholar]
  8. SLEIN M. W., LOGAN G. F., Jr Partial purification and properties of two phospholipases of Bacillus cereus. J Bacteriol. 1963 Feb;85:369–381. doi: 10.1128/jb.85.2.369-381.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Slein M. W., Logan G. F. Characterization of the Phospholipases of Bacillus cereus and Their Effects on Erythrocytes, Bone, and Kidney Cells. J Bacteriol. 1965 Jul;90(1):69–81. doi: 10.1128/jb.90.1.69-81.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Slein M. W., Logan G. F. MECHANISM OF ACTION OF THE TOXIN OF BACILLUS ANTHRACIS II. : Alkaline Phosphatasemia Produced by Culture Filtrates of Various Bacilli. J Bacteriol. 1962 Feb;83(2):359–369. doi: 10.1128/jb.83.2.359-369.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Thomas L., Kinne R. Studies on the arrangement of aminopeptidase and alkaline phosphatase in the microvilli of isolated brush border of rat kidney. Biochim Biophys Acta. 1972 Jan 17;255(1):114–125. doi: 10.1016/0005-2736(72)90013-2. [DOI] [PubMed] [Google Scholar]
  12. Wachsmuth E. D., Hiwada K. Alkaline phosphatase from pig kidney. Method of purification and molecular properties. Biochem J. 1974 Jul;141(1):273–282. doi: 10.1042/bj1410273. [DOI] [PMC free article] [PubMed] [Google Scholar]

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