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. 1977 Dec 15;168(3):529–532. doi: 10.1042/bj1680529

Amino acid transport in brush-border-membrane vesicles isolated from human small intestine.

H Lücke, W Haase, H Murer
PMCID: PMC1183801  PMID: 606251

Abstract

Uptake of L-alanine and L-phenylalanine by purified bursh-border-membrane vesicles isolated from human small intestine was investigated by using a rapid-filtration technique. L-Alanine entered the same osmotically reactive space as D-glucose, indicating that transport into the vesicle rather than binding to the membranes was being observed. The uptake rate for L-alanine was higher in the presence of a Na+ gradient than in the presence of a K+ gradient. In the presence of a Na+ gradient, the lipophilic anion SCN- caused an increase in L-alanine transport, whereas the nearly impermeant SO42- anion decreased the uptake of L-alanine compared with its uptake in the presence of Cl-. The uptake of L-phenylalanine into the brush-border-membrane vesicle was also stimulated by Na+. The results indicate co-transport of Na+ and neutral amino acids inthe human intestinal brush-border membrane.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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