Abstract
A procedure involving affinity chromatography on cellulose was developed for separating enzymic components of the cellulase complex. Cellobiase, carboxymethylcellulase, component C2 and cellobiohydrolase are adsorbed with increasing tenacity, and released, as highly purified components, as the ionic strength of the eluent is decreased.
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Selected References
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- Berghem L. E., Pettersson L. G., Axiö-Fredriksson U. B. The mechanism of enzymatic cellulose degradation. Purification and some properties of two different 1,4beta-glucan glucanohydrolases from Trichoderma viride. Eur J Biochem. 1976 Jan 15;61(2):621–630. doi: 10.1111/j.1432-1033.1976.tb10058.x. [DOI] [PubMed] [Google Scholar]
- HALLIWELL G. HYDROLYSIS OF FIBROUS COTTON AND REPRECIPITATED CELLULOSE BY CELLULOLYTIC ENZYMES FROM SOIL MICRO-ORGANISMS. Biochem J. 1965 Apr;95:270–281. doi: 10.1042/bj0950270. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HALLIWELL G. The action of cellulolytic enzymes from Myrothecium verrucaria. Biochem J. 1961 Apr;79:185–192. doi: 10.1042/bj0790185. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Halliwell G., Griffin M. The nature and mode of action of the cellulolytic component C1 of Trichoderma koningii on native cellulose. Biochem J. 1973 Dec;135(4):587–594. doi: 10.1042/bj1350587. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Halliwell G., Riaz M. Interactions between components of the cellulase complex of Trichoderma koningii on native substrates. Arch Mikrobiol. 1971;78(4):295–309. doi: 10.1007/BF00412270. [DOI] [PubMed] [Google Scholar]
- Halliwell G., Riaz M. The formation of short fibres from native cellulose by components of Trichoderma koningii cellulase. Biochem J. 1970 Jan;116(1):35–42. doi: 10.1042/bj1160035. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Li L. H., Flora R. M., King K. W. Individual roles of cellulase components derived from Trichoderma viride. Arch Biochem Biophys. 1965 Aug;111(2):439–447. doi: 10.1016/0003-9861(65)90207-9. [DOI] [PubMed] [Google Scholar]