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. 1978 Jan 15;170(1):57–61. doi: 10.1042/bj1700057

The mode of action of alpha sarcin and a novel assay of the puromycin reaction.

A N Hobden, E Cundliffe
PMCID: PMC1183860  PMID: 629783

Abstract

A new technique was developed for measuring the amount of peptidyl-tRNA in a protein-synthesizing system in vitro. By this technique the course of the puromycin reaction may be followed and the modes of action of various inhibitors of protein synthesis readily determined. We conclude that the polypeptide alpha sarcin inhibits the binding of aminoacyl-tRNA into the ribosomal 'A' site, that sparsomycin inhibits the peptidyl transferase reaction and that cycloheximide may block translocation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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