Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1978 Feb 15;170(2):227–233. doi: 10.1042/bj1700227

Vitamin D metabolism and expression in rats fed on low-calcium and low-phosphorus diets

Samuel Edelstein *,§, Dina Noff *, Levana Sinai *, Arie Harell *, Jules B Puschett †, Ellis E Golub ‡, Felix Bronner ‡,∥
PMCID: PMC1183888  PMID: 205207

Abstract

1. Cholecalciferol, radioactively labelled with both 14C and 3H, was administered weekly for 7 weeks to rats that had been depleted of vitamin D for 4 weeks before repletion with the radioactive vitamin. This permitted measurement of the steady-state effect on vitamin D metabolism of low-calcium and low-phosphorus regimens, as compared with a normal mineral intake. These dietary manoeuvres were carried out during the last 3 weeks of repletion. Cholecalciferol, 25-hydroxycholecalciferol and 1,25-dihydroxycholecalciferol were determined in plasma, intestine, kidney and bone. Ca2+-binding-protein content was measured in intestine and kidneys of comparable animals. 2. In rats on the low-calcium diets, 1,25-dihydroxycholecalciferol concentration was elevated in plasma, bone, kidney and intestine, and intestinal Ca2+-binding protein was increased to over twice the concentration found in the control animals. 3. The low-phosphorus regimens led to a decrease in plasma phosphate and 1,25-dihydroxycholecalciferol in all tissues studied, for the latter to the point where it was undetectable in plasma and bone. Intestinal and renal concentrations of Ca2+-binding protein were unchanged in the low-phosphate-intake group and decreased in the very-low-phosphate-intake group. 4. It is concluded that in the rat, unlike in the chick, hypophosphataemia is not associated with a stimulation of the production of 1,25-dihydroxycholecalciferol or its expression in the synthesis of Ca2+-binding protein. Therefore the plasma phosphate concentration does not appear to be directly involved in the regulation of the functional metabolism of vitamin D.

Full text

PDF
227

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Bar A., Hurwitz S., Edelstein S. Response of renal calcium-binding protein. Independence of kidney vitamin D hydroxylation. Biochim Biophys Acta. 1975 Nov 10;411(1):106–112. doi: 10.1016/0304-4165(75)90289-5. [DOI] [PubMed] [Google Scholar]
  3. Bar A., Wasserman R. H. Duodenal calcium binding protein in the chick: a new bioassay for vitamin D. J Nutr. 1974 Sep;104(9):1202–1207. doi: 10.1093/jn/104.9.1202. [DOI] [PubMed] [Google Scholar]
  4. Bronner F., Freund T. Intestinal CaBP: a new quantitive index of vitamin D deficiency in the rat. Am J Physiol. 1975 Sep;229(3):689–694. doi: 10.1152/ajplegacy.1975.229.3.689. [DOI] [PubMed] [Google Scholar]
  5. Bronner F. Vitamin D deficiency and rickets. Am J Clin Nutr. 1976 Nov;29(11):1307–1314. doi: 10.1093/ajcn/29.11.1307. [DOI] [PubMed] [Google Scholar]
  6. Castillo L., Tanaka Y., DeLuca H. F. The mobilization of bone mineral by 1,25-dihydroxyvitamin D3 in hypophosphatemic rats. Endocrinology. 1975 Oct;97(4):995–999. doi: 10.1210/endo-97-4-995. [DOI] [PubMed] [Google Scholar]
  7. Chen T. C., Castillo L., Korycka-Dahl M., DeLuca H. F. Role of vitamin D metabolites in phosphate transport of rat intestine. J Nutr. 1974 Aug;104(8):1056–1060. doi: 10.1093/jn/104.8.1056. [DOI] [PubMed] [Google Scholar]
  8. Cuisnier-Gleizes P., Thomasset M., Sainteny-Debove F., Mathieu H. Phosphorus deficiency, parathyroid hormone and bone resorption in the growing rat. Calcif Tissue Res. 1976 Jun 14;20(3):235–249. doi: 10.1007/BF02546412. [DOI] [PubMed] [Google Scholar]
  9. DeLuca H. F. Regulation of vitamin D metabolism. Life Sci. 1975 Nov 1;17(9):1351–1358. doi: 10.1016/0024-3205(75)90151-4. [DOI] [PubMed] [Google Scholar]
  10. DeLuca H. F. The kidney as an endocrine organ for the production of 1,25-dihydroxyvitamin D 3 , a calcium-mobilizing hormone. N Engl J Med. 1973 Aug 16;289(7):359–365. doi: 10.1056/NEJM197308162890710. [DOI] [PubMed] [Google Scholar]
  11. Edelstein S., Harell A., Bar A., Hurwitz S. The functional metabolism of vitamin D in chicks fed low-calcium and low-phosphorus diets. Biochim Biophys Acta. 1975 Apr 7;385(2):438–442. doi: 10.1016/0304-4165(75)90376-1. [DOI] [PubMed] [Google Scholar]
  12. Emtage J. S., Lawson D. E., Kodicek E. Vitamin D-induced synthesis of mRNA for calcium-binding protein. Nature. 1973 Nov 9;246(5428):100–101. doi: 10.1038/246100a0. [DOI] [PubMed] [Google Scholar]
  13. Fraser D. R., Kodicek E. Regulation of 25-hydroxycholecalciferol-1-hydroxylase activity in kidney by parathyroid hormone. Nat New Biol. 1973 Feb 7;241(110):163–166. doi: 10.1038/newbio241163a0. [DOI] [PubMed] [Google Scholar]
  14. Freund T., Bronner F. Regulation of intestinal calcium-binding protein calcium intake in the rat. Am J Physiol. 1975 Mar;228(3):861–869. doi: 10.1152/ajplegacy.1975.228.3.861. [DOI] [PubMed] [Google Scholar]
  15. Freund T., Bronner F. Stimulation in vitro by 1,25-dihydroxy-vitamin D3 of intestinal cell calcium uptake and calcium-binding protein. Science. 1975 Dec 26;190(4221):1300–1302. doi: 10.1126/science.1198113. [DOI] [PubMed] [Google Scholar]
  16. HUMMEL J. P., DREYER W. J. Measurement of protein-binding phenomena by gel filtration. Biochim Biophys Acta. 1962 Oct 8;63:530–532. doi: 10.1016/0006-3002(62)90124-5. [DOI] [PubMed] [Google Scholar]
  17. Haussler M., Hughes M., Baylink D., Littledike E. T., Cork D., Pitt M. Influence of phosphate depletion on the biosynthesis and circulating level of 1alpha,25-dihydroxyvitamin D. Adv Exp Med Biol. 1977;81:233–250. doi: 10.1007/978-1-4613-4217-5_24. [DOI] [PubMed] [Google Scholar]
  18. Hermsdorf C. L., Bronner F. Vitamin D-dependent calcium-binding protein from rat kidney. Biochim Biophys Acta. 1975 Feb 27;379(2):553–561. doi: 10.1016/0005-2795(75)90161-0. [DOI] [PubMed] [Google Scholar]
  19. Hughes M. R., Brumbaugh P. F., Hussler M. R., Wergedal J. E., Baylink D. J. Regulation of serum 1alpha,25-dihydroxyvitamin D3 by calcium and phosphate in the rat. Science. 1975 Nov 7;190(4214):578–580. doi: 10.1126/science.1188357. [DOI] [PubMed] [Google Scholar]
  20. Hurwitz S., Stacey R. E., Bronner F. Role of vitamin D in plasma calcium regulation. Am J Physiol. 1969 Feb;216(2):254–262. doi: 10.1152/ajplegacy.1969.216.2.254. [DOI] [PubMed] [Google Scholar]
  21. Lawson D. E., Pelc B., Bell P. A., Wilson P. W., Kodicek E. Synthesis of (1,2- 3 H 2 )cholecalciferol and metabolism of (4- 14 C,1,2- 3 H 2 )- and (4- 14 C,1- 3 H)-cholecalciferol in rachitic rats and chicks. Biochem J. 1971 Feb;121(4):673–682. doi: 10.1042/bj1210673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Norman A. W., Henry H. 1,25-Dihydroxycholecalciferol--a hormonally active form of vitamin D. Recent Prog Horm Res. 1974;30(0):431–480. [PubMed] [Google Scholar]
  23. Puschett J. B., Beck W. S., Jr Parathyroid hormone and 25-hydroxy vitamin D3: synergistic and antagonistic effects on renal phosphate transport. Science. 1975 Oct 31;190(4213):473–475. doi: 10.1126/science.1166316. [DOI] [PubMed] [Google Scholar]
  24. Puschett J. B., Fernandez P. C., Boyle I. T., Gray R. W., Omdahl J. L., DeLuca H. F. The acute renal tubular effects of 1,25-dihydroxycholecalciferol. Proc Soc Exp Biol Med. 1972 Oct;141(1):379–384. doi: 10.3181/00379727-141-36781. [DOI] [PubMed] [Google Scholar]
  25. Puschett J. B., Moranz J., Kurnick W. S. Evidence for a direct action of cholecalciferol and 25-hydroxycholecalciferol on the renal transport of phosphate, sodium, and calcium. J Clin Invest. 1972 Feb;51(2):373–385. doi: 10.1172/JCI106823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Ribovich M. L., DeLuca H. F. The influence of dietary calcium and phosphorus on intestinal calcium transport in rats given vitamin D metabolites. Arch Biochem Biophys. 1975 Oct;170(2):529–535. doi: 10.1016/0003-9861(75)90148-4. [DOI] [PubMed] [Google Scholar]
  27. Tanaka Y., Frank H., DeLuca H. F. Intestinal calcium transport: stimulation by low phosphorus diets. Science. 1973 Aug 10;181(4099):564–566. doi: 10.1126/science.181.4099.564. [DOI] [PubMed] [Google Scholar]
  28. Thomasset M., Cuisinier-Gleizes P., Mathieu H. Duodenal calcium-binding protein (CaBP) and phosphorus deprivation in growing rats. Biomedicine. 1976 Dec 5;25(9):345–349. [PubMed] [Google Scholar]
  29. Weber J. C., Pons V., Kodicek E. The localization of 1,25-dihydroxycholecalciferol in bone cell nuclei of rachitic chicks. Biochem J. 1971 Nov;125(1):147–153. doi: 10.1042/bj1250147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Weisman Y., Sapir R., Harell A., Edelstein S. Maternal-perinatal interrelationships of vitamin D metabolism in rats. Biochim Biophys Acta. 1976 Apr 23;428(2):388–395. doi: 10.1016/0304-4165(76)90046-5. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES