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. 1978 May 1;171(2):453–459. doi: 10.1042/bj1710453

Lysine residue 240 of human serum albumin is involved in high-affinity binding of bilirubin.

C Jacobsen
PMCID: PMC1183975  PMID: 656055

Abstract

Bilirubin can be coupled covalently to albumin by using water-soluble carbodi-imide as coupling reagent. The optimal specificity in the attachment of bilirubin to the high-affinity site on the albumin molecule was obtained by treating an albumin-bilirubin complex with carbodi-imide in low concentrations and for a short period. The product was reduced, carboxymethylated and digested with trypsin. By fractionation on Sephadex G-50 (superfine grade) a peptide fraction containing most of the bilirubin label was isolated. Further purification by paper chromatography gave one peptide, consisting of residues 240-258. The peptide containined a single lysine residue, 240, and had an intact disulphide bridge. The results indicate that bilirubin is bound to lysine residue 240 at its high-affinity site on human serum albumin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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