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. 1978 May 1;171(2):505–508. doi: 10.1042/bj1710505

The preparation of liposomes bearing human (HLA) transplantation antigens.

M J Turner, A R Sanderson
PMCID: PMC1183984  PMID: 656061

Abstract

The incorporation of HLA antigens into liposomes is described. At and above the lipidphase-transition temperature, between 50 and 80% of the added antigenic activity may be incorporated into liposomes in the form of multilamellar vesicles. The antigen appears to be asymmetrically orientated with respect to the lipid bilayer.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison A. G., Gregoriadis G. Liposomes as immunological adjuvants. Nature. 1974 Nov 15;252(5480):252–252. doi: 10.1038/252252a0. [DOI] [PubMed] [Google Scholar]
  2. Grey H. M., Kubo R. T., Colon S. M., Poulik M. D., Cresswell P., Springer T., Turner M., Strominger J. L. The small subunit of HL-A antigens is beta 2-microglobulin. J Exp Med. 1973 Dec 1;138(6):1608–1612. doi: 10.1084/jem.138.6.1608. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Howard I. K., Sage H. J., Stein M. D., Young N. M., Leon M. A., Dyckes D. F. Studies on a phytohemagglutinin from the lentil. II. Multiple forms of Lens culinaris hemagglutinin. J Biol Chem. 1971 Mar 25;246(6):1590–1595. [PubMed] [Google Scholar]
  4. LOWRY O. H., ROBERTS N. R., LEINER K. Y., WU M. L., FARR A. L. The quantitative histochemistry of brain. I. Chemical methods. J Biol Chem. 1954 Mar;207(1):1–17. [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. March S. C., Parikh I., Cuatrecasas P. A simplified method for cyanogen bromide activation of agarose for affinity chromatography. Anal Biochem. 1974 Jul;60(1):149–152. doi: 10.1016/0003-2697(74)90139-0. [DOI] [PubMed] [Google Scholar]
  8. Morris R. J., Letarte-Muirhead M., Williams A. F. Analysis in deoxycholate of three antigenic specificities associated with the rat Thy-1 molecule. Eur J Immunol. 1975 Apr;5(4):282–285. doi: 10.1002/eji.1830050413. [DOI] [PubMed] [Google Scholar]
  9. Racker E., Chien T. F., Kandrach A. A cholate-dilution procedure for the reconstitution of the Ca++ pump, 32Pi--ATP exchange, and oxidative phosphorylation. FEBS Lett. 1975 Sep 1;57(1):14–18. doi: 10.1016/0014-5793(75)80141-4. [DOI] [PubMed] [Google Scholar]
  10. SANDERSON A. R. APPLICATIONS OF ISO-IMMUNE CYTOLYSIS USING RADIOLABELLED TARGET CELLS. Nature. 1964 Oct 17;204:250–253. doi: 10.1038/204250a0. [DOI] [PubMed] [Google Scholar]
  11. Sanderson A. R., Welsh K. I. Properties of histocompatibility (HL-A) determinants. 3. The independent preciptation and epitope valence of soluble HL-A determinants. Transplantation. 1974 Sep;18(3):197–205. doi: 10.1097/00007890-197409000-00001. [DOI] [PubMed] [Google Scholar]
  12. Snary D., Goodfellow P., Hayman M. J., Bodmer W. F., Crumpton M. J. Subcellular separation and molecular nature of human histocompatibility antigens (HL-A). Nature. 1974 Feb 15;247(5441):457–461. doi: 10.1038/247457a0. [DOI] [PubMed] [Google Scholar]
  13. Springer T. A., Strominger J. L. Detergent-soluble HLA antigens contain a hydrophilic region at the COOH-terminus and a penultimate hydrophobic region. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2481–2485. doi: 10.1073/pnas.73.7.2481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Springer T. A., Strominger J. L., Mann D. Partial purification of detergent-soluble HL-A antigen and its cleavage by papain. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1539–1543. doi: 10.1073/pnas.71.4.1539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Walsh F. S., Crumpton M. J. Orientation of cell-surface antigens in the lipid bilayer of lymphocyte plasma membrane. Nature. 1977 Sep 22;269(5626):307–311. doi: 10.1038/269307a0. [DOI] [PubMed] [Google Scholar]
  16. Wickner W. Asymmetric orientation of phage M13 coat protein in Escherichia coli cytoplasmic membranes and in synthetic lipid vesicles. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1159–1163. doi: 10.1073/pnas.73.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]

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