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. 1978 Jun 1;171(3):539–542. doi: 10.1042/bj1710539

The proton exchange of the pro-S hydrogen atom at C-1 in dihydroxyacetone phosphate and D-fructose 1,6-bisphosphate catalysed by class-I and class-II aldolases.

A Galdes, H A Hill
PMCID: PMC1183997  PMID: 352341

Abstract

The efficacy of class-I and class-II aldolases in catalysing the C-1 proton exchange in fructose 1,6-bisphosphate and dihydroxyacetone phosphate was investigated. The rate of this reaction was at least two orders of magnitude slower in class-II than in the class-I aldolases. It is suggested that this difference reflects the formation of different intermediates in the reactions catalysed by the two classes of aldolase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Gray G. R., Barker R. Studies on the substrates of D-fructose 1,6-diphosphate aldolase in solution. Biochemistry. 1970 Jun 9;9(12):2454–2462. doi: 10.1021/bi00814a010. [DOI] [PubMed] [Google Scholar]
  2. Hartman F. C., Brown J. P. Affinity labeling of a previously undetected essential lysyl residue in class I fructose bisphosphate aldolase. J Biol Chem. 1976 May 25;251(10):3057–3062. [PubMed] [Google Scholar]
  3. Hartman F. C., Suh B., Welch M. H., Barker R. Inactivation of class I fructose diphosphate aldolases by the substrate analog N-bromoacetylethanolamine phosphate. J Biol Chem. 1973 Dec 10;248(23):8233–8239. [PubMed] [Google Scholar]
  4. Hartman F. C., Welch M. H. Identification of the histidyl residue of rabbit muscle aldolase alkylated by N-bromoacetylethanolamine phosphate. Biochem Biophys Res Commun. 1974 Mar 15;57(1):85–92. doi: 10.1016/s0006-291x(74)80360-8. [DOI] [PubMed] [Google Scholar]
  5. Hill H. A., Lobb R. R., Sharp S. L., Stokes A. M., Harris J. I., Jack R. S. Metal-replacement studies in Bacillus stearothermophilus aldolase and a comparison of the mechanisms of class I and class II aldolases. Biochem J. 1976 Mar 1;153(3):551–560. doi: 10.1042/bj1530551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hoffee P., Lai C. Y., Pugh E. L., Horecker B. L. The function of histidine residues in rabbit muscle aldolase. Proc Natl Acad Sci U S A. 1967 Jan;57(1):107–113. doi: 10.1073/pnas.57.1.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ingram J. M. Fructose diphosphate aldolase from Fusarium oxysporum f. lycopersici. 3. Studies on the mechanism of reaction. Can J Biochem. 1967 Dec;45(12):1909–1917. doi: 10.1139/o67-224. [DOI] [PubMed] [Google Scholar]
  8. Lobb R. R., Stokes A. M., Hill H. A., Riordan J. F. A functional arginine residue in rabbit-muscle aldolase. Eur J Biochem. 1976 Nov 15;70(2):517–522. doi: 10.1111/j.1432-1033.1976.tb11043.x. [DOI] [PubMed] [Google Scholar]
  9. Lobb R. R., Stokes A. M., Hill H. A., Riordan J. F. Arginine as the C-1 phosphate binding site in rabbit muscle aldolase. FEBS Lett. 1975 Jun 1;54(1):70–72. doi: 10.1016/0014-5793(75)81070-2. [DOI] [PubMed] [Google Scholar]
  10. Lowe G., Pratt R. F. Proton exchange of the pro-S hydrogen at C-1 in dihydroxyacetone phosphate, D-fructose 1,6-bisphosphate and D-fructose 1-phosphate catalysed by rabbit-muscle aldolase. Eur J Biochem. 1976 Jun 15;66(1):95–104. doi: 10.1111/j.1432-1033.1976.tb10429.x. [DOI] [PubMed] [Google Scholar]
  11. RICHARDS O. C., RUTTER W. J. Comparative properties of yeast and muscle aldolase. J Biol Chem. 1961 Dec;236:3185–3192. [PubMed] [Google Scholar]
  12. RICHARDS O. C., RUTTER W. J. Preparation and properties of yeast aldolase. J Biol Chem. 1961 Dec;236:3177–3184. [PubMed] [Google Scholar]
  13. ROSE I. A. Mechanism of C-H bond cleavage in aldolase and isomerase reactions. Brookhaven Symp Biol. 1962 Dec;15:293–309. [PubMed] [Google Scholar]
  14. ROSE I. A., O'CONNELL E. L., MEHLER A. H. MECHANISM OF THE ALDOLASE REACTION. J Biol Chem. 1965 Apr;240:1758–1765. [PubMed] [Google Scholar]
  15. ROSE I. A., RIEDER S. V. Studies on the mechanism on the aldolase reaction; isotope exchange reactions of muscle and yeast aldolase. J Biol Chem. 1958 Mar;231(1):315–329. [PubMed] [Google Scholar]
  16. RUTTER W. J. EVOLUTION OF ALDOLASE. Fed Proc. 1964 Nov-Dec;23:1248–1257. [PubMed] [Google Scholar]
  17. Willard J. M., Gibbs M. Role of Aldolase in Photosynthesis. II Demonstration of Aldolase Types in Photosynthetic Organisms. Plant Physiol. 1968 May;43(5):793–798. doi: 10.1104/pp.43.5.793. [DOI] [PMC free article] [PubMed] [Google Scholar]

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