Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1978 Jun 1;171(3):781–786. doi: 10.1042/bj1710781

The pseudouridine contents of the ribosomal ribonucleic acids of three vertebrate species. Numerical correspondence between pseudouridine residues and 2'-O-methyl groups is not always conserved.

D G Hughes, B E Maden
PMCID: PMC1184027  PMID: 666737

Abstract

The pseudouridine contents of the rRNA species of HeLa cells, mouse L-cells and Xenopus laevis cultured kidney cells were examined. Pseudouridine, like 2'-O-methylation, was found to occur relatively frequently in each of the high-molecular-weight rRNA species. However, the numerical data do not support the idea that there is a general one-to-one relationship between pseudoridine residues and 2'-O-methyl groups in vertebrate rRNA.

Full text

PDF
781

Images in this article

782-1PLATE 1
on p.782-1

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altman S., Smith J. D. Tyrosine tRNA precursor molecule polynucleotide sequence. Nat New Biol. 1971 Sep 8;233(36):35–39. doi: 10.1038/newbio233035a0. [DOI] [PubMed] [Google Scholar]
  2. Amaldi F., Attardi G. Partial sequence analysis of ribosomal RNA from HeLa cells. I. Oligonucleotide pattern of 28 s and 18 s RNA after pancreatic ribonuclease digestion. J Mol Biol. 1968 May 14;33(3):737–755. doi: 10.1016/0022-2836(68)90317-3. [DOI] [PubMed] [Google Scholar]
  3. Birnstiel M., Speirs J., Purdom I., Jones K., Loening U. E. Properties and composition of the isolated ribosomal DNA satellite of Xenopus laevis. Nature. 1968 Aug 3;219(5153):454–463. doi: 10.1038/219454a0. [DOI] [PubMed] [Google Scholar]
  4. Brand R. C., Klootwijk J., Planta R. J., Maden B. E. Biosynthesis of a hypermodified nucleotide in Saccharomyces carlsbergensis 17S and HeLa-cell 18S ribosomal ribonucleic acid. Biochem J. 1978 Jan 1;169(1):71–77. doi: 10.1042/bj1690071. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DUNN D. B. Additional components in ribonucleic acid of rat-liver fractions. Biochim Biophys Acta. 1959 Jul;34:286–288. doi: 10.1016/0006-3002(59)90274-4. [DOI] [PubMed] [Google Scholar]
  6. GLITZ D. G., DEKKER C. A. THE PURIFICATION AND PROPERTIES OF RIBONUCLEIC ACID FROM WHEAT GERM. Biochemistry. 1963 Nov-Dec;2:1185–1192. doi: 10.1021/bi00906a002. [DOI] [PubMed] [Google Scholar]
  7. Hughes D. G., Hughes S., Maden B. E. The pseudouridine content of hela cell ribosomal RNA. FEBS Lett. 1976 Dec 31;72(2):304–308. doi: 10.1016/0014-5793(76)80992-1. [DOI] [PubMed] [Google Scholar]
  8. Jeanteur P., Amaldi F., Attardi G. Partial sequence analysis of ribosomal RNA from HeLa cells. II. Evidence for sequences of non-ribosmal type in 45 and 32 s ribosomal RNA precursors. J Mol Biol. 1968 May 14;33(3):757–775. doi: 10.1016/0022-2836(68)90318-5. [DOI] [PubMed] [Google Scholar]
  9. Khan M. S., Maden B. E. Nucleotide sequences within the ribosomal ribonucleic acids of HeLa cells, Xenopus laevis and chick embryo fibroblasts. J Mol Biol. 1976 Feb 25;101(2):235–254. doi: 10.1016/0022-2836(76)90375-2. [DOI] [PubMed] [Google Scholar]
  10. Khan M. S., Salim M., Maden B. E. Extensive homologies between the methylated nucleotide sequences in several vertebrate ribosomal ribonucleic acids. Biochem J. 1978 Mar 1;169(3):531–542. doi: 10.1042/bj1690531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Klootwijk J., Planta R. J. Analysis of the methylation sites in yeast ribosomal RNA. Eur J Biochem. 1973 Nov 15;39(2):325–333. doi: 10.1111/j.1432-1033.1973.tb03130.x. [DOI] [PubMed] [Google Scholar]
  12. Lane B. G., Tamaoki T. Studies of the chain termini and alkali-stable dinucleotide sequences in 16 s and 28 s ribosomal RNA from L cells. J Mol Biol. 1967 Jul 28;27(2):335–348. doi: 10.1016/0022-2836(67)90024-1. [DOI] [PubMed] [Google Scholar]
  13. Loening U. E. Molecular weights of ribosomal RNA in relation to evolution. J Mol Biol. 1968 Dec;38(3):355–365. doi: 10.1016/0022-2836(68)90391-4. [DOI] [PubMed] [Google Scholar]
  14. Maden B. E., Forbes J., de Jonge P., Klootwijk J. Presence of a hypermodified nucleotide in HeLa cell 18 S and Saccharomyces carlsbergensis 17 S ribosomal RNAs. FEBS Lett. 1975 Nov 1;59(1):60–63. doi: 10.1016/0014-5793(75)80341-3. [DOI] [PubMed] [Google Scholar]
  15. Maden B. E.H., Forbes J. Standard and non standard products in combined T(1) plus pancreatic RNAase fingerprints of HeLa cell rRNA and its precursors. FEBS Lett. 1972 Dec 15;28(3):289–292. doi: 10.1016/0014-5793(72)80733-6. [DOI] [PubMed] [Google Scholar]
  16. Maden B. E., Robertson J. S. Demonstration of the "5-8 S" ribosomal sequence in HeLa cell ribosomal precursor RNA. J Mol Biol. 1974 Aug 5;87(2):227–235. doi: 10.1016/0022-2836(74)90145-4. [DOI] [PubMed] [Google Scholar]
  17. Maden B. E., Salim M. The methylated nucleotide sequences in HELA cell ribosomal RNA and its precursors. J Mol Biol. 1974 Sep 5;88(1):133–152. doi: 10.1016/0022-2836(74)90299-x. [DOI] [PubMed] [Google Scholar]
  18. Nazar R. N., Sitz T. O., Busch H. Structural analyses of mammalian ribosomal ribonucleic acid and its precursors. Nucleotide sequence of ribosomal 5.8 S ribonucleic acid. J Biol Chem. 1975 Nov 25;250(22):8591–8597. [PubMed] [Google Scholar]
  19. Nichols J. L., Lane B. G. In vivo incorporation of methyl groups into the ribose of Escherichia coli ribosomal RNA. J Mol Biol. 1967 Dec 28;30(3):477–489. doi: 10.1016/0022-2836(67)90363-4. [DOI] [PubMed] [Google Scholar]
  20. Petermann M. L., Pavlovec A. The subunits and structural ribonucleic acids of Jensen sarcoma ribosomes. Biochim Biophys Acta. 1966 Feb 21;114(2):264–276. doi: 10.1016/0005-2787(66)90308-x. [DOI] [PubMed] [Google Scholar]
  21. Saponara A. G., Enger M. D. The isolation from ribonucleic acid of substituted uridines containing alpha-aminobutyrate moieties derived from methionine. Biochim Biophys Acta. 1974 Apr 27;349(1):61–77. doi: 10.1016/0005-2787(74)90009-4. [DOI] [PubMed] [Google Scholar]
  22. Slack J. M., Loening U. E. 5'-Ends of ribosomal and ribosomal precursor RNAs form Xenopus laevis. Eur J Biochem. 1974 Mar 15;43(1):59–67. doi: 10.1111/j.1432-1033.1974.tb03384.x. [DOI] [PubMed] [Google Scholar]
  23. Stanley W. M., Jr, Bock R. M. Isolation and physical properties of the ribosomal ribonucleic acid of Escherichia coli. Biochemistry. 1965 Jul;4(7):1302–1311. doi: 10.1021/bi00883a014. [DOI] [PubMed] [Google Scholar]
  24. Thomas G., Gordon J., Rogg H. N4-Acetylcytidine. A previously unidentified labile component of the small subunit of eukaryotic ribosomes. J Biol Chem. 1978 Feb 25;253(4):1101–1105. [PubMed] [Google Scholar]
  25. WYATT G. R. The purine and pyrimidine composition of deoxypentose nucleic acids. Biochem J. 1951 May;48(5):584–590. doi: 10.1042/bj0480584. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wellauer P. K., Dawid I. B. Secondary structure maps of RNA: processing of HeLa ribosomal RNA. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2827–2831. doi: 10.1073/pnas.70.10.2827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wellauer P. K., Dawid I. B. Secondary structure maps of ribosomal RNA and DNA. I. Processing of Xenopus laevis ribosomal RNA and structure of single-stranded ribosomal DNA. J Mol Biol. 1974 Oct 25;89(2):379–395. doi: 10.1016/0022-2836(74)90526-9. [DOI] [PubMed] [Google Scholar]
  28. Willems M., Wagner E., Laing R., Penman S. Base composition of ribosomal RNA precursors in the HeLa cell nucleolus: further evidence of non-conservative processing. J Mol Biol. 1968 Mar 14;32(2):211–220. doi: 10.1016/0022-2836(68)90005-3. [DOI] [PubMed] [Google Scholar]
  29. YU C. T., ALLEN F. W. Studies on an isomer of uridine isolated from ribonucleic acids. Biochim Biophys Acta. 1959 Apr;32:393–406. doi: 10.1016/0006-3002(59)90612-2. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES