Abstract
Uptake of arsenite by rat liver mitochondria is energy-dependent, as shown by comparing values without and with either uncoupling agent or respiratory inhibitor present. The uptake is inhibited by mersalyl and N-ethylmaleimide, which can be used as 'stopping' agents to obtain uptake kinetics. At 20 degrees C the process is nearly complete in 1 min. The relation between the quantity in the energized mitochondria and the applied concentration corresponds to at least two different modes of binding of the arsenite. Competition occurs between arsenite and other anions (for example, phosphate) for intramitochondrial accumulation.
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Selected References
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- Csillag A. The effect of arsenite on rat-liver mitochondria. FEBS Lett. 1971 Oct 1;17(2):342–344. doi: 10.1016/0014-5793(71)80183-7. [DOI] [PubMed] [Google Scholar]
- FLUHARTY A. L., SANADI D. R. On the mechanism of oxidative phosphorylation. II. Effects of arsenite alone and in combination with 2,3-dimercaptopropanol. J Biol Chem. 1961 Oct;236:2772–2778. [PubMed] [Google Scholar]
- FLUHARTY A. L., SANADI D. R. On the mechanism of oxidative phosphorylation. IV. Mitochondrial swelling caused by arsenite in combination with 2,3-dimercaptopropanol and by cadmium ion. Biochemistry. 1962 Mar;1:276–281. doi: 10.1021/bi00908a014. [DOI] [PubMed] [Google Scholar]
- Harris E. J., Bangham J. A., Wimhurst J. M. The dependence on energy and K+ ions of the accumulation of glutamate by rat liver mitochondria. Arch Biochem Biophys. 1973 Sep;158(1):236–241. doi: 10.1016/0003-9861(73)90617-6. [DOI] [PubMed] [Google Scholar]
- Harris E. J., Manger J. R. Intramitochondrial substrate concentration as a factor controlling metabolism. The role of interanion competition. Biochem J. 1968 Sep;109(2):239–246. doi: 10.1042/bj1090239. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harris E. J., Tate C., Manger J. R., Bangham J. A. The effects of colloids on the appearance and substrate permeability of rat liver mitochondria. J Bioenerg. 1971 Aug;2(3):221–232. doi: 10.1007/BF01648917. [DOI] [PubMed] [Google Scholar]
- Hassinen I., Hallman M. Comparison of the effects of disulfiram and dimercaptopropanol arsenite on mitochondrial structure and function. Biochem Pharmacol. 1967 Nov;16(11):2155–2161. doi: 10.1016/0006-2952(67)90014-7. [DOI] [PubMed] [Google Scholar]
- Palmieri F., Klingenberg M. Inhibition of respiration under the control of azide uptake by mitochondria. Eur J Biochem. 1967 Jun;1(4):439–446. doi: 10.1007/978-3-662-25813-2_60. [DOI] [PubMed] [Google Scholar]