Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1978 Apr 1;171(1):263–266. doi: 10.1042/bj1710263

Problems encountered in measuring the activity of phosphatidate phosphohydrolase.

R G Sturton, D N Brindley
PMCID: PMC1184155  PMID: 206266

Abstract

The measurement of phosphate release from phosphatidate overestimates the microsomal activity of phosphatidate phosphohydrolase from rat liver, since phosphate is also produced via the glycerol phosphate that results from the deacylation of phosphatidate. The determination of phosphate production can be a reliable assay for the soluble phosphatidate phosphohydrolase in rat liver, because the glycerol phosphate formed is not hydrolysed under the conditions used.

Full text

PDF
261

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Bjerve K. S., Daae L. N., Bremer J. The selective loss of lysophospholipids in some commonly used lipid-extraction procedures. Anal Biochem. 1974 Mar;58(1):238–245. doi: 10.1016/0003-2697(74)90463-1. [DOI] [PubMed] [Google Scholar]
  3. Blank M. L., Wykle R. L., Alper S., Snyder F. Microsomal synthesis of the ether analogs of triacylglycerols. Acyl CoA:alkylacylglycerol and acyl CoA:alk-i-enylacylglycerol acyltransferases in tumors and liver. Biochim Biophys Acta. 1974 Jun 26;348(3):397–403. doi: 10.1016/0005-2760(74)90219-7. [DOI] [PubMed] [Google Scholar]
  4. Bowley M., Cooling J., Burditt S. L., Brindley D. N. The effects of amphiphilic cationic drugs and inorganic cations on the activity of phosphatidate phosphohydrolase. Biochem J. 1977 Sep 1;165(3):447–454. doi: 10.1042/bj1650447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brindley D. N., Bowley M. Drugs affecting the synthesis of glycerides and phospholipids in rat liver. The effects of clofibrate, halofenate, fenfluramine, amphetamine, cinchocaine, chlorpromazine, demethylimipramine, mepyramine and some of their derivatives. Biochem J. 1975 Jun;148(3):461–469. doi: 10.1042/bj1480461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Brindley D. N. Some aspects of the physiological and pharmacological control of the synthesis of triacylglycerols and phospholipids. Int J Obes. 1978;2(1):7–16. [PubMed] [Google Scholar]
  7. COLEMAN R., HUEBSCHER G. Metabolism of phospholipids. V. Studies of phosphatidic acid phosphatase. Biochim Biophys Acta. 1962 Jan 29;56:479–490. doi: 10.1016/0006-3002(62)90600-5. [DOI] [PubMed] [Google Scholar]
  8. Caras I., Shapiro B. Partial purification and properties of microsomal phosphatidate phosphohydrolase from rat liver. Biochim Biophys Acta. 1975 Nov 21;409(2):201–211. doi: 10.1016/0005-2760(75)90154-x. [DOI] [PubMed] [Google Scholar]
  9. Fallon H. J., Lamb R. G., Jamdar S. C. Phosphatidate phosphohydrolase and the regulation of glycerolipid biosynthesis. Biochem Soc Trans. 1977;5(1):37–40. doi: 10.1042/bst0050037. [DOI] [PubMed] [Google Scholar]
  10. Hajra A. K., Seguin E. B., Agranoff B. W. Rapid labeling of mitochondrial lipids by labeled orthophosphate and adenosine triphosphate. J Biol Chem. 1968 Apr 10;243(7):1609–1616. [PubMed] [Google Scholar]
  11. Hosaka K., Yamashita S., Numa S. Partial purification, properties, and subcellulsr distribution of rat liver phosphatidate phosphatase. J Biochem. 1975 Mar;77(3):501–509. doi: 10.1093/oxfordjournals.jbchem.a130751. [DOI] [PubMed] [Google Scholar]
  12. Jamdar S. C., Fallon H. J. Glycerolipid synthesis in rat adipose tissue. II. Properties and distribution of phosphatidate phosphatase. J Lipid Res. 1973 Sep;14(5):517–524. [PubMed] [Google Scholar]
  13. Johnston J. M., Rao G. A., Lowe P. A., Schwarz B. E. The nature of the stimulatory role of the supernatant fraction on triglyceride synthesis by the alpha-Glycerophosphate pathway. Lipids. 1967 Jan;2(1):14–20. doi: 10.1007/BF02531994. [DOI] [PubMed] [Google Scholar]
  14. Kako K. J., Patterson S. D. Phosphatidate phosphohydrolase and palmitoyl-coenzyme A hydrolase in cardiac subcellular fractions of hyperthyroid rabbits and cardiomyopathic hamsters. Biochem J. 1975 Nov;152(2):313–323. doi: 10.1042/bj1520313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pritchard P. H., Bowley M., Burditt S. L., Cooling J., Glenny H. P., Lawson N., Sturton R. G., Brindley D. N. The effects of acute ethanol feeding and of chronic benfluorex administration on the activities of some enzymes of glycerolipid synthesis in rat liver and adipose tissue. Biochem J. 1977 Sep 15;166(3):639–642. doi: 10.1042/bj1660639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Renkonen O. Mono- and dimethyl phosphatidates from different subtypes of choline and ethanolamine glycerophosphatides. Biochim Biophys Acta. 1968 Jan 10;152(1):114–135. doi: 10.1016/0005-2760(68)90014-3. [DOI] [PubMed] [Google Scholar]
  17. Savolainen M. J. Stimulation of hepatic phosphatidate phosphohydrolase activity by a single dose of ethanol;. Biochem Biophys Res Commun. 1977 Mar 21;75(2):511–518. doi: 10.1016/0006-291x(77)91071-3. [DOI] [PubMed] [Google Scholar]
  18. Shapiro B., Tzur R. The control of lipid biosynthesis in liver particles. Ann N Y Acad Sci. 1968 Nov 21;149(2):784–790. doi: 10.1111/j.1749-6632.1968.tb53835.x. [DOI] [PubMed] [Google Scholar]
  19. Smith M. E., Sedgwick B., Brindley D. N., Hübscer G. The role of phosphatidate phosphohydrolase in glyceride biosynthesis. Eur J Biochem. 1967 Dec;3(1):70–77. doi: 10.1111/j.1432-1033.1967.tb19499.x. [DOI] [PubMed] [Google Scholar]
  20. Sturton R. G., Brindley D. N. Factors controlling the activities of phosphatidate phosphohydrolase and phosphatidate cytidylyltransferase. The effects of chlorpromazine, demethylimipramine, cinchocaine, norfenfluramine, mepyramine and magnesium ions. Biochem J. 1977 Jan 15;162(1):25–32. doi: 10.1042/bj1620025. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tzur R., Shapiro B. Phosphatidic acid metabolism in rat liver microsomes. Eur J Biochem. 1976 Apr 15;64(1):301–305. doi: 10.1111/j.1432-1033.1976.tb10301.x. [DOI] [PubMed] [Google Scholar]
  22. Whiting P. H., Bowley M., Sturton R. G., Pritchard P. H., Brindley D. N., Hawthorne J. N. The effect of chronic diabetes, induced by streptozotocin, on the activities of some enzymes of glycerolipid synthesis in rat liver. Biochem J. 1977 Nov 15;168(2):147–153. doi: 10.1042/bj1680147. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES