Table 2. List of antibodies used.
| Application | Antibody | Species | Dilution | Company (Catalog #) |
|---|---|---|---|---|
| Western Blot | SMN | Mouse | 1:2 500 | BD Transduction (610647) |
| Western Blot | Alpha-tubulin | Rabbit | 1:10 000 | Abcam (ab4074) |
| Western Blot | Alpha-tubulin | Mouse | 1:10 000 | Sigma-Aldrich (CP06) |
| Western Blot | Heme oxygenase-1 (HO) | Rabbit | 1:10 000 | Abcam (ab68477) |
| Western Blot | P62 | Mouse | 1:1 000 | Abcam (ab56416) |
| Western Blot | Transferrin | Rabbit | 1:1 000 | Abcam (ab82411) |
| Western Blot | Goat anti-mouse IR Dye 800 CW | 1:5 000 | LI-COR (925–32210) | |
| Western Blot | Goat anti-mouse IR Dye 680 R | 1:5 000 | LI-COR (926–68070) | |
| Western Blot | Goat anti-rabbit IR Dye 800 CW | 1:5 000 | LI-COR (925–32211) | |
| Western Blot | Goat anti-rabbit IR Dye 680 R | 1:5 000 | LI-COR (926–68071) | |
| IHC | ChAT | Goat | 1:100 | Millipore (AB144P) |
| IHC | Donkey anti-goat Alexa Fluor 555 | 1:200 | Invitrogen (A21432) | |
| IHC | TRITC conjugated bungarotoxin | N/A | 1:1 000 | Invitrogen (T1175) |
| IHC | Neurofilament (NF-M) | Mouse | 1:100 | (Developmental Studies Hybridoma Bank, P12839) |
| IHC | Synaptic vesicle glycoprotein 2 A (SV2A) | Mouse | 1:250 | (Developmental Studies Hybridoma Bank, Q7L0J3) |
| IHC | Goat anti-mouse Alexa Fluor 488 | 1:250 | Invitrogen (A11001) | |
| IHC | Glucagon | Mouse | 1:200 | Abcam (ab10988) |
| IHC (Parafin) IHC (Frozen) |
Cre Recombinase (D7L7L) | Rabbit | 1:500 1:200 |
Cell Signaling (15036) |
| IHC | Insulin | Rabbit | 1:50 | Abcam (ab181547) |
| IHC | Goat anti-mouse Alexa Fluor 555 | 1:500 | Invitrogen (A21422) | |
| IHC | Goat anti-rabbit Alexa Fluor 488 | 1:500 | Invitrogen (A11034) |