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. 1997 Apr;72(4):1659–1667. doi: 10.1016/S0006-3495(97)78811-8

Differences in the physical properties of lipid monolayers and bilayers on a spherical solid support.

F M Linseisen 1, M Hetzer 1, T Brumm 1, T M Bayerl 1
PMCID: PMC1184359  PMID: 9083669

Abstract

A monolayer of 1,2-dipalmitoyl-d62-glycero-3-phosphocholine (DPPC-d62) coated onto silanized silica beads (spherical supported monolayer: SSM) is studied by 2H-NMR and DSC. The results are compared with those obtained from a single bilayer on the same solid support (spherical supported vesicles: SSV) and from multilamellar vesicles (MLV). The phase transition temperature (Tm) of the SSMs is significantly higher than that of the bilayer systems and the extent of this difference depends on the lipid density in the monolayer that is determined during its preparation. 2H-NMR reveals a gel and fluid phase coexistence in the SSM transition region. A comparison of the 2H-NMR line shapes suggests the presence of highly curved structures for the fluid phase of the SSM samples. From a comparison of SSM and SSV transverse relaxation in the fluid phase we can conclude that the lateral diffusion coefficient D1 in supported monolayers is similar to that in bilayers.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bayerl T. M., Bloom M. Physical properties of single phospholipid bilayers adsorbed to micro glass beads. A new vesicular model system studied by 2H-nuclear magnetic resonance. Biophys J. 1990 Aug;58(2):357–362. doi: 10.1016/S0006-3495(90)82382-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brumm T., Möps A., Dolainsky C., Brückner S., Bayerl T. M. Macroscopic orientation effects in broadline NMR-spectra of model membranes at high magnetic field strength: A method preventing such effects. Biophys J. 1992 Apr;61(4):1018–1024. doi: 10.1016/S0006-3495(92)81909-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Davis J. H. Deuterium magnetic resonance study of the gel and liquid crystalline phases of dipalmitoyl phosphatidylcholine. Biophys J. 1979 Sep;27(3):339–358. doi: 10.1016/S0006-3495(79)85222-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davis J. H. The description of membrane lipid conformation, order and dynamics by 2H-NMR. Biochim Biophys Acta. 1983 Mar 21;737(1):117–171. doi: 10.1016/0304-4157(83)90015-1. [DOI] [PubMed] [Google Scholar]
  5. Helm C. A., Möhwald H., Kjaer K., Als-Nielsen J. Phospholipid monolayers between fluid and solid states. Biophys J. 1987 Sep;52(3):381–390. doi: 10.1016/S0006-3495(87)83226-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Johnson S. J., Bayerl T. M., Weihan W., Noack H., Penfold J., Thomas R. K., Kanellas D., Rennie A. R., Sackmann E. Coupling of spectrin and polylysine to phospholipid monolayers studied by specular reflection of neutrons. Biophys J. 1991 Nov;60(5):1017–1025. doi: 10.1016/S0006-3495(91)82139-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lafleur M., Fine B., Sternin E., Cullis P. R., Bloom M. Smoothed orientational order profile of lipid bilayers by 2H-nuclear magnetic resonance. Biophys J. 1989 Nov;56(5):1037–1041. doi: 10.1016/S0006-3495(89)82749-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MacKay A. L. A proton NMR moment study of the gel and liquid-crystalline phases of dipalmitoyl phosphatidylcholine. Biophys J. 1981 Aug;35(2):301–313. doi: 10.1016/S0006-3495(81)84791-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Morrow M. R., Whitehead J. P., Lu D. Chain-length dependence of lipid bilayer properties near the liquid crystal to gel phase transition. Biophys J. 1992 Jul;63(1):18–27. doi: 10.1016/S0006-3495(92)81579-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Möhwald H. Phospholipid and phospholipid-protein monolayers at the air/water interface. Annu Rev Phys Chem. 1990;41:441–476. doi: 10.1146/annurev.pc.41.100190.002301. [DOI] [PubMed] [Google Scholar]
  11. Naumann C., Brumm T., Bayerl T. M. Phase transition behavior of single phosphatidylcholine bilayers on a solid spherical support studied by DSC, NMR and FT-IR. Biophys J. 1992 Nov;63(5):1314–1319. doi: 10.1016/S0006-3495(92)81708-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Needham D., Evans E. Structure and mechanical properties of giant lipid (DMPC) vesicle bilayers from 20 degrees C below to 10 degrees C above the liquid crystal-crystalline phase transition at 24 degrees C. Biochemistry. 1988 Oct 18;27(21):8261–8269. doi: 10.1021/bi00421a041. [DOI] [PubMed] [Google Scholar]
  13. Okamura E., Umemura J., Takenaka T. Orientation studies of hydrated dipalmitoylphosphatidylcholine multibilayers by polarized FTIR-ATR spectroscopy. Biochim Biophys Acta. 1990 Jun 11;1025(1):94–98. doi: 10.1016/0005-2736(90)90195-t. [DOI] [PubMed] [Google Scholar]
  14. Reinl H. M., Bayerl T. M. Interaction of myelin basic protein with single bilayers on a solid support: an NMR, DSC and polarized infrared ATR study. Biochim Biophys Acta. 1993 Sep 19;1151(2):127–136. doi: 10.1016/0005-2736(93)90095-h. [DOI] [PubMed] [Google Scholar]
  15. Reinl H. M., Bayerl T. M. Lipid transfer between small unilamellar vesicles and single bilayers on a solid support: self-assembly of supported bilayers with asymmetric lipid distribution. Biochemistry. 1994 Nov 29;33(47):14091–14099. doi: 10.1021/bi00251a018. [DOI] [PubMed] [Google Scholar]
  16. Rowe E. S. Thermodynamic reversibility of phase transitions. Specific effects of alcohols on phosphatidylcholines. Biochim Biophys Acta. 1985 Mar 14;813(2):321–330. doi: 10.1016/0005-2736(85)90248-2. [DOI] [PubMed] [Google Scholar]
  17. Vaknin D., Kjaer K., Als-Nielsen J., Lösche M. Structural properties of phosphatidylcholine in a monolayer at the air/water interface: Neutron reflection study and reexamination of x-ray reflection measurements. Biophys J. 1991 Jun;59(6):1325–1332. doi: 10.1016/S0006-3495(91)82347-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Zasadzinski J. A., Viswanathan R., Madsen L., Garnaes J., Schwartz D. K. Langmuir-Blodgett films. Science. 1994 Mar 25;263(5154):1726–1733. doi: 10.1126/science.8134836. [DOI] [PubMed] [Google Scholar]

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