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. 1997 Mar;72(3):1193–1203. doi: 10.1016/S0006-3495(97)78767-8

Noise analysis of ion channels in non-space-clamped cables: estimates of channel parameters in olfactory cilia.

H P Larsson 1, S J Kleene 1, H Lecar 1
PMCID: PMC1184503  PMID: 9138566

Abstract

Ion channels in the cilia of olfactory neurons are part of the transduction machinery of olfaction. Odorant stimuli have been shown to induce a biphasic current response, consisting of a cAMP-activated current and a Ca(2+)-activated Cl- current. We have developed a noise analysis method to study ion channels in leaky cables, such as the olfactory cilium, under non-space-clamp conditions. We performed steady-state noise analysis on ligand-induced currents in excised cilia, voltage-clamped at input and internally perfused with cAMP or Ca2+. The cAMP-activated channels analyzed by this method gave results similar to those of single-channel recordings (gamma = 8.3 pS). Single-channel currents have not yet been recorded for the Ca(2+)-activated Cl- channels. Using our noise analysis method, we estimate a unit conductance, gamma = 0.8 pS, for these channels. The density of channels was found to be approximately 70 channels/micron2 for both channel species.

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Selected References

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