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. 1997 Mar;72(3):1258–1263. doi: 10.1016/S0006-3495(97)78772-1

Shape modification of phospholipid vesicles induced by high pressure: influence of bilayer compressibility.

L Beney 1, J M Perrier-Cornet 1, M Hayert 1, P Gervais 1
PMCID: PMC1184508  PMID: 9138571

Abstract

Giant vesicles composed of pure egg yolk phosphatidylcholine (EYPC) or containing cholesterol (28 mol%) have been studied during a high hydrostatic pressure treatment to 285 MPa by microscopic observation. During pressure loading the vesicles remain spherical. A shape transition consisting of budding only occurs on the cholesterol-free vesicles during pressure release. The decrease in the volume delimited by the pure EYPC bilayer between 0.1 and 285 MPa was found to be 16% of its initial volume, whereas the bulk compression of water in this pressure range is only 10%. So the compression at 285 MPa induced a water exit from the pure EYPC vesicle. The shape transition of the EYPC vesicle during pressure release is attributed to an increase in its area-to-volume ratio caused by the loss of its water content during compression. Because bulk compression of the cholesterol-containing vesicle is close to that of water, no water transfer would be induced across the bilayer and the vesicle remains spherical during the pressure release.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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