Abstract
1. An extracellular enzyme has been isolated from cultures of the bacterium growing on agar or porphyran. 2. Partial purification of the enzyme has been achieved by precipitation with ammonium sulphate followed by gel filtration on DEAE-Sephadex A-50. 3. The optimum conditions for the enzyme acting on porphyran are pH7·2 and a temperature of 40–41°. 4. The enzyme has an endoaction, producing a rapid decrease in viscosity of agar or porphyran solutions with little development of reducing power. 5. From the action of the enzyme on various polysaccharides, it is concluded that the enzyme is specific for the agarose structure.
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Selected References
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- Duckworth M., Turvey J. R. The extracellular agarase from a Cytophaga species. Biochem J. 1968 Sep;109(2):6P–6P. doi: 10.1042/bj1090006pa. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REES D. A. Enzymic desulphation of porphyran. Biochem J. 1961 Sep;80:449–453. doi: 10.1042/bj0800449. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STORVICK W. O., KING K. W. The complexity and mode of action of the cellulase system of Cellvibrio gilvus. J Biol Chem. 1960 Feb;235:303–307. [PubMed] [Google Scholar]
- SWARTZ M. N., GORDON N. Agarase from an agar-digesting bacterium. J Bacteriol. 1959 Apr;77(4):403–409. doi: 10.1128/jb.77.4.403-409.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stanier R. Y. Studies on Marine Agar-Digesting Bacteria. J Bacteriol. 1941 Oct;42(4):527–559. doi: 10.1128/jb.42.4.527-559.1941. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Turvey J. R., Christison J. The enzymic degradation of porphyran. Biochem J. 1967 Oct;105(1):317–321. doi: 10.1042/bj1050317. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Turvey J. R., Christison J. The hydrolysis of algal galactans by enzymes from a Cytophaga species. Biochem J. 1967 Oct;105(1):311–316. doi: 10.1042/bj1050311. [DOI] [PMC free article] [PubMed] [Google Scholar]
- YAPHE W. The use of agarase from Pseudomonas atlantica in the identification of agar in marine algae (Rhodophyceae). Can J Microbiol. 1957 Dec;3(7):987–993. doi: 10.1139/m57-109. [DOI] [PubMed] [Google Scholar]