Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1969 Jun;113(1):207–214. doi: 10.1042/bj1130207

Regulation of glycolysis and l-glycerol 3-phosphate concentration in rat epididymal adipose tissue in vitro. Role of phosphofructokinase

M L Halperin 1, R M Denton 1
PMCID: PMC1184621  PMID: 4308837

Abstract

1. Attempts were made to define the role of phosphofructokinase in glycolytic control and the factors regulating the concentration of l-glycerol 3-phosphate in rat epididymal fat pads incubated in vitro. 2. Glycolysis rates were altered by anoxia or by additions of insulin, adrenaline or both to the incubation medium, and the changes in rate were related to changes in the steady-state concentrations of hexose phosphates, adenine nucleotides, l-glycerol 3-phosphate and citrate in the whole tissue. Measurements were also made of the lactate/pyruvate concentration ratio in the medium after incubation. 3. The mass-action ratios of phosphofructokinase, calculated from the whole-tissue concentrations of products and substrates, were less than 0·1% of the value of the ratio at pH7·4 at equilibrium. 4. Only in the presence of adrenaline could the observed stimulation of glycolytic flux be related to a possible activation of phosphofructokinase since, in this situation, the concentration of one substrate, fructose 6-phosphate, was not altered and the concentration of the other, ATP, was decreased. Increased glycolytic flux in the presence of insulin may be explained by an observed increase in the concentration of the substrate, fructose 6-phosphate. Under anaerobic conditions, glycolytic flux was decreased but this did not appear to be the result of inhibition of phosphofructokinase, since the concentrations of both substrates, fructose 6-phosphate and ATP, were decreased. The changes in glycolytic flux with insulin and anoxia may be secondary to changes in the rate of glucose uptake. 5. Changes in l-glycerol 3-phosphate concentration appear to be related both to changes in the concentration of dihydroxyacetone phosphate and to changes in the NADH/NAD+ concentration ratio in the cytoplasm. They do not seem to be related directly to alterations in glycolytic rate.

Full text

PDF
207

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BALL E. G., JUNGAS R. L. STUDIES ON THE METABOLISM OF ADIPOSE TISSUE. XIII. THE EFFECT OF ANAEROBIC CONDITIONS AND DIETARY REGIME ON THE RESPONSE TO INSULIN AND EPINEPHRINE. Biochemistry. 1963 May-Jun;2:586–592. doi: 10.1021/bi00903a035. [DOI] [PubMed] [Google Scholar]
  2. Butcher R. W., Ho R. J., Meng H. C., Sutherland E. W. Adenosine 3',5'-monophosphate in biological materials. II. The measurement of adenosine 3',5'-monophosphate in tissues and the role of the cyclic nucleotide in the lipolytic response of fat to epinephrine. J Biol Chem. 1965 Nov;240(11):4515–4523. [PubMed] [Google Scholar]
  3. CAHILL G. F., Jr, LEBOEUF B., RENOLD A. E. Studies on rat adipose tissue in vitro. III. Synthesis of glycogen and glyceride-glycerol. J Biol Chem. 1959 Oct;234:2540–2543. [PubMed] [Google Scholar]
  4. CROFFORD O. B., RENOLD A. E. GLUCOSE UPTAKE BY INCUBATED RAT EPIDIDYMAL ADIPOSE TISSUE. CHARACTERISTICS OF THE GLUCOSE TRANSPORT SYSTEM AND ACTION OF INSULIN. J Biol Chem. 1965 Aug;240:3237–3244. [PubMed] [Google Scholar]
  5. CROFFORD O. B., RENOLD A. E. GLUCOSE UPTAKE BY INCUBATED RAT EPIDIDYMAL ADIPOSE TISSUE. RATE-LIMITING STEPS AND SITE OF INSULIN ACTION. J Biol Chem. 1965 Jan;240:14–21. [PubMed] [Google Scholar]
  6. Denton R. M., Halperin M. L. The control of fatty acid and triglyceride synthesis in rat epididymal adipose tissue. Roles of coenzyme A derivatives, citrate and L-glycerol 3-phosphate. Biochem J. 1968 Nov;110(1):27–38. doi: 10.1042/bj1100027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Denton R. M., Randle P. J. Citrate and the regulation of adipose-tissue phosphofructokinase. Biochem J. 1966 Aug;100(2):420–423. doi: 10.1042/bj1000420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Denton R. M., Randle P. J. Measurement of flow of carbon atoms from glucose and glycogen glucose to glyceride glycerol and glycerol in rat heart and epididymal adipose tissue. Effects of insulin, adrenaline and alloxan-diabetes. Biochem J. 1967 Aug;104(2):423–434. doi: 10.1042/bj1040423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Denton R. M., Yorke R. E., Randle P. J. Measurement of concentrations of metabolites in adipose tissue and effects of insulin, alloxan-diabetes and adrenaline. Biochem J. 1966 Aug;100(2):407–419. doi: 10.1042/bj1000407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. FLATT J. P., BALL E. G. STUDIES ON THE METABOLISM OF ADIPOSE TISSUE. XV. AN EVALUATION OF THE MAJOR PATHWAYS OF GLUCOSE CATABOLISM AS INFLUENCED BY INSULIN AND EPINEPHRINE. J Biol Chem. 1964 Mar;239:675–685. [PubMed] [Google Scholar]
  11. FRITZ I. B. Factors influencing the rates of long-chain fatty acid oxidation and synthesis in mammalian systems. Physiol Rev. 1961 Jan;41:52–129. doi: 10.1152/physrev.1961.41.1.52. [DOI] [PubMed] [Google Scholar]
  12. Hernández A., Sols A. Transport and phosphorylation of sugars in adipose tissue. Biochem J. 1963 Jan;86(1):166–172. doi: 10.1042/bj0860166. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Katz J., Landau B. R., Bartsch G. E. The pentose cycle, triose phosphate isomerization, and lipogenesis in rat adipose tissue. J Biol Chem. 1966 Feb 10;241(3):727–740. [PubMed] [Google Scholar]
  14. SHORT D. J., PARKES A. S. Feeding and breeding of laboratory animals; a compound diet for monkeys. J Hyg (Lond) 1949 Jun;47(2):209–212. doi: 10.1017/s0022172400014480. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. WINEGRAD A. I., RENOLD A. E. Studies on rat adipose tissue in vitro. I. Effects of insulin on the metabolism of glucose, pyruvate, and acetate. J Biol Chem. 1958 Aug;233(2):267–272. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES