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. 1969 Jun;113(2):325–332. doi: 10.1042/bj1130325

Lipid peroxide formation in microsomes. The role of non-haem iron

E D Wills 1
PMCID: PMC1184639  PMID: 4390102

Abstract

1. Metal ion-chelating agents such as EDTA, o-phenanthroline or desferrioxamine inhibit lipid peroxide formation when rat liver microsomes prepared from homogenates made in pure sucrose are incubated with ascorbate or NADPH. 2. Microsomes treated with metal ion-chelating agents do not form peroxide on incubation unless inorganic iron (Fe2+ or Fe3+) in a low concentration is added subsequently. No other metal ion can replace inorganic iron adequately. 3. Microsomes prepared from sucrose homogenates containing EDTA (1mm) do not form lipid peroxide on incubation with ascorbate or NADPH unless Fe2+ is added. Washing the microsomes with sucrose after preparation restores most of the capacity to form lipid peroxide. 4. Lipid peroxide formation in microsomes prepared from sucrose is stimulated to a small extent by inorganic iron but to a greater extent if adenine nucleotides, containing iron compounds as a contaminant, are added. 5. The iron contained in normal microsome preparations exists in haem and in non-haem forms. One non-haem component in which the iron may be linked to phosphate is considered to be essential for both the ascorbate system and NADPH system that catalyse lipid peroxidation in microsomes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. HENRY R. J., SOBEL C., CHIAMORI N. On the determination of serum iron and iron-binding capacity. Clin Chim Acta. 1958 Nov;3(6):523–530. doi: 10.1016/0009-8981(58)90004-4. [DOI] [PubMed] [Google Scholar]
  2. Hochstein P., Nordenbrand K., Ernster L. Evidence for the involvement of iron in the ADP-activated peroxidation of lipids in microsomes and mitochondria. Biochem Biophys Res Commun. 1964;14:323–328. doi: 10.1016/s0006-291x(64)80004-8. [DOI] [PubMed] [Google Scholar]
  3. MCKNIGHT R. C., HUNTER F. E., Jr, OEHLERT W. H. MITOCHONDRIAL MEMBRANE GHOSTS PRODUCED BY LIPID PEROXIDATION INDUCED BY FERROUS ION. I. PRODUCTION AND GENERAL MORPHOLOGY. J Biol Chem. 1965 Aug;240:3439–3445. [PubMed] [Google Scholar]
  4. WILLS E. D. MECHANISMS OF LIPID PEROXIDE FORMATION IN TISSUES. ROLE OF METALS AND HAEMATIN PROTEINS IN THE CATALYSIS OF THE OXIDATION UNSATURATED FATTY ACIDS. Biochim Biophys Acta. 1965 Apr 5;98:238–251. doi: 10.1016/0005-2760(65)90118-9. [DOI] [PubMed] [Google Scholar]
  5. Wills E. D. Lipid peroxide formation in microsomes. General considerations. Biochem J. 1969 Jun;113(2):315–324. doi: 10.1042/bj1130315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Wills E. D. Lipid peroxide formation in microsomes. Relationship of hydroxylation to lipid peroxide formation. Biochem J. 1969 Jun;113(2):333–341. doi: 10.1042/bj1130333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Wills E. D. Mechanisms of lipid peroxide formation in animal tissues. Biochem J. 1966 Jun;99(3):667–676. doi: 10.1042/bj0990667. [DOI] [PMC free article] [PubMed] [Google Scholar]

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