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. 1969 Aug;113(5):757–763. doi: 10.1042/bj1130757

The separation and distribution of simple and condensed leucoanthocyanins of the tea plant (Camellia sinensis L.)

G I Forrest 1,*, D S Bendall 1
PMCID: PMC1184765  PMID: 5821007

Abstract

1. Leucoanthocyanin monomers of high mobilities in aqueous solvents on thinlayer chromatograms, assumed to be structurally simple, were characteristic of mature bulky tissues, whereas members of lower mobility were confined to young vegetative and floral tissues. 2. Flavylogens were separated by gel filtration on Sephadex columns into monomeric, oligomeric and polymeric fractions. 3. The polymeric fraction from young brown stems was heterogeneous, one-half having a molecular weight of about 3400, one-third a molecular weight between 3600 and 17000, and the remainder a molecular weight of over 17000. 4. Leaves had low flavylogen concentrations; only monomers were present. Stem tissues were rich in polymers, which increased with the age of the young stem and decreased inwards through the wood. The maximal flavylogen concentrations were in the phloem and cambium from mature stems, where all three fractions were richly present. The periderm tissue and, to a lesser extent, the seed coat were characterized by a very high polymer/monomer ratio, exhibiting a much higher degree of polymerization than the wood. Root tissues contained high concentrations of monomers. 5. In general, there was an inverse correlation between the extent of polymerization and the complexity of the monomers present. 6. The results are in favour of the thesis that the function of the flavanols is, after polymerization to condensed tannins, to impregnate dead structural tissues and thereby to protect them from infection and decay.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Creasy L. L., Swain T. Structure of condensed tannins. Nature. 1965 Oct 9;208(5006):151–153. doi: 10.1038/208151a0. [DOI] [PubMed] [Google Scholar]
  2. Drewes S. E., Roux D. G. Condensed tannins. 15. Interrelationships of flavonoid components in wattle-bark extract. Biochem J. 1963 Apr;87(1):167–172. doi: 10.1042/bj0870167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Forrest G. I., Bendall D. S. The distribution of polyphenols in the tea plant (Camellia sinensis L.). Biochem J. 1969 Aug;113(5):741–755. doi: 10.1042/bj1130741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HEBB C., HILL K. J. Pseudocholinesterase in pacinian corpuscles. Nature. 1955 Apr 2;175(4457):597–597. doi: 10.1038/175597a0. [DOI] [PubMed] [Google Scholar]
  5. HILLIS W. E., CARLE A. The formation of phenolic substances in Eucalyptus gigantea and Eucalyptus sieberiana. Biochem J. 1960 Mar;74:607–615. doi: 10.1042/bj0740607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. King H. G. Leucoanthocyanins from the leaves of European larch (Larix decidua, Mill). Nature. 1966 Aug 27;211(5052):944–945. doi: 10.1038/211944a0. [DOI] [PubMed] [Google Scholar]
  7. ROUX D. G., EVELYN S. R. Condensed tannins. I. A study of complex leucoanthocyanins present in condensed tannins. Biochem J. 1958 Aug;69(4):530–538. doi: 10.1042/bj0690530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. ROUX D. G., MAIHS E. A., PAULUS E. Condensed tannins. 9. Distribution of flavonoid compounds in the heartwoods and barks of some interrelated wattles. Biochem J. 1961 Apr;78:834–839. doi: 10.1042/bj0780834. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. ROUX D. G., PAULUS E. Condensed tannis. 12. Polymeric leucofisetinidin tannns from the heartwood of Acacia mearnsii. Biochem J. 1962 Feb;82:320–324. doi: 10.1042/bj0820320. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Roux D. G., Evelyn S. R. Condensed tannins. 2. Biogenesis of condensed tannins based on leucoanthocyanins. Biochem J. 1958 Oct;70(2):344–349. doi: 10.1042/bj0700344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Roux D. G., Evelyn S. R. Condensed tannins. 4. The distribution and deposition of tannins in the heartwoods of Acacia mollissima and Schinopsis spp. Biochem J. 1960 Jul;76(1):17–23. doi: 10.1042/bj0760017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Somers T. C. Wine tannins--isolation of condensed flavonoid pigments by gel-filtration. Nature. 1966 Jan 22;209(5021):368–370. doi: 10.1038/209368a0. [DOI] [PubMed] [Google Scholar]

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