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. 1969 Aug;114(1):1–3. doi: 10.1042/bj1140001

The catabolism of cholesterol in vitro. Formation of 3α,7α,12α-trihydroxy-5β-cholestanoic acid from cholesterol by rat liver

D Mendelsohn 1, L Mendelsohn 1
PMCID: PMC1184787  PMID: 5810058

Abstract

1. Both 25-d- and 25-l-3α,7α,12α-trihydroxy-5β-cholestanoic acid were isolated from the gall-bladder bile of Crocodylus niloticus. 2. The catabolism of cholesterol to 25-d- and 25-l-3α,7α,12α-trihydroxy-5β-cholestanoic acid respectively was studied by using a rat liver preparation in vitro. The results show that rat liver can metabolize cholesterol to both forms of 3α,7α,12α-trihydroxy-5β-cholestanoic acid. However, a preference was noted for the formation from [4-14C]cholesterol of 3α,7α,12α-trihydroxy-5β-cholestanoic acid (25-d), which was isolated from the incubations with a specific radioactivity about four times that of 3α,7α,12α-trihydroxy-5β-cholestanoic acid (25-l). 3. The results indicate that 3α,7α,12α-trihydroxy-5β-cholestanoic acid is a normal intermediate in the biosynthesis of bile acids from cholesterol in the rat.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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