Abstract
1. The incorporation into protein, and the accumulation into the free amino acid pools, of radioactive l-leucine and glycine was studied in rat extensor digitorum longus muscle. 2. The tissue was incubated first with 14C-labelled and then with 3H-labelled amino acid. 3. The experimental results were consistent with a model based on the premise that the amino acids in protein were incorporated directly from the extracellular pool.
Full text
PDF







Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ASKONAS B. A., HUMPHREY J. H. Formation of specific antibodies and gamma-globulin in vitro; a study of the synthetic ability of various tissues from rabbits immunized by different methods. Biochem J. 1958 Feb;68(2):252–261. doi: 10.1042/bj0680252. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CASTLES J. J., WOOL I. G., MOYER A. N. THE EFFECT OF DIABETES ON AMINO ACID ACCUMULATION AND PROTEIN SYNTHESIS IN ISOLATED RAT DIAPHRAGM. Biochim Biophys Acta. 1965 May 4;100:609–612. doi: 10.1016/0304-4165(65)90036-x. [DOI] [PubMed] [Google Scholar]
- HENDLER R. W. A model for protein synthesis. Nature. 1962 Mar 3;193:821–823. doi: 10.1038/193821a0. [DOI] [PubMed] [Google Scholar]
- HENDLER R. W. ISOLATION OF "LIPOPEPTIDES" FROM HEN OVIDUCT. Biochim Biophys Acta. 1963 Sep 10;74:659–666. doi: 10.1016/0006-3002(63)91417-3. [DOI] [PubMed] [Google Scholar]
- Harris E. J., Manchester K. L. The effects of potassium ions and denervation on protein synthesis and the transport of amino acids in muscle. Biochem J. 1966 Oct;101(1):135–145. doi: 10.1042/bj1010135. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KIPNIS D. M., CORI C. F. Studies of tissue permeability. III. The effect of insulin on pentose uptake by the diaphragm. J Biol Chem. 1957 Feb;224(2):681–693. [PubMed] [Google Scholar]
- KIPNIS D. M., REISS E., HELMREICH E. Functional heterogeneity of the intracellular amino acid pool in mammalian cells. Biochim Biophys Acta. 1961 Aug 19;51:519–524. doi: 10.1016/0006-3002(61)90608-4. [DOI] [PubMed] [Google Scholar]
- KOSTYO J. L. SEPARATION OF THE EFFECTS OF GROWTH HORMONE ON MUSCLE AMINO ACID TRANSPORT AND PROTEIN SYNTHESIS. Endocrinology. 1964 Jul;75:113–119. doi: 10.1210/endo-75-1-113. [DOI] [PubMed] [Google Scholar]
- KRAHL M. E. STIMULATION OF PEPTIDE SYNTHESIS IN ADIPOSE TISSUE BY INSULIN WITHOUT GLUCOSE. Am J Physiol. 1964 Mar;206:618–620. doi: 10.1152/ajplegacy.1964.206.3.618. [DOI] [PubMed] [Google Scholar]
- MANCHESTER K. L. Insulin and incorporation of amino acids into protein of muscle. Cellular amino acid levels and aminoisobutyric acid uptake. Biochem J. 1961 Oct;81:135–147. doi: 10.1042/bj0810135. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MANCHESTER K. L., WOOL I. G. INSULIN AND INCORPORATION OF AMINO ACIDS INTO PROTEIN OF MUSCLE. 1. ACCUMULATION AND INCORPORATION STUDIES WITH THE PERFUSED RAT HEART. Biochem J. 1963 Nov;89:202–209. doi: 10.1042/bj0890202. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Manchester K. L. Some factors affecting the response of muscle to insulin. Biochem J. 1966 Mar;98(3):711–719. doi: 10.1042/bj0980711. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OXENDER D. L., CHRISTENSEN H. N. DISTINCT MEDIATING SYSTEMS FOR THE TRANSPORT OF NEUTRAL AMINO ACIDS BY THE EHRLICH CELL. J Biol Chem. 1963 Nov;238:3686–3699. [PubMed] [Google Scholar]
- RIGGS T. R., WALKER L. M. SOME RELATIONS BETWEEN ACTIVE TRANSPORT OF FREE AMINO ACIDS INTO CELLS AND THEIR INCORPORATION INTO PROTEIN. J Biol Chem. 1963 Aug;238:2663–2668. [PubMed] [Google Scholar]
- ROBERTS L. E., BENSCH K., CARTER C. E. AMINO ACID-ACTIVATED ADENOSINE TRIPHOSPHATE-PYROPHOSPHATE EXCHANGE BY MODIFIED ESCHERICHIA COLI CELLS. Biochim Biophys Acta. 1964 Aug 19;90:291–300. doi: 10.1016/0304-4165(64)90192-8. [DOI] [PubMed] [Google Scholar]
- ROSENBERG L. E., BERMAN M., SEGAL S. Studies of the kinetics of amino acid transport, incorporation into portein and oxidation in kidney-cortex slices. Biochim Biophys Acta. 1963 Jun 4;71:664–675. doi: 10.1016/0006-3002(63)91140-5. [DOI] [PubMed] [Google Scholar]
- Riggs T. R., Pan M. W., Feng H. W. Transport of amino acids into the estrogen-primed uterus. I. General characteristics of the uptake in vitro. Biochim Biophys Acta. 1968 Jan 3;150(1):92–103. doi: 10.1016/0005-2736(68)90012-6. [DOI] [PubMed] [Google Scholar]
- Roscoe J. P., Eaton M. D., Choy G. C. Inhibition of protein synthesis in Krebs 2 ascites cells and cell-free systems by phenylalanine and its effect on leucine and lysine in the amino acid pool. Biochem J. 1968 Oct;109(4):507–515. doi: 10.1042/bj1090507. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SEGAL S. HORMONES, AMINO-ACID TRANSPORT AND PROTEIN SYNTHESIS. Nature. 1964 Jul 4;203:17–19. doi: 10.1038/203017a0. [DOI] [PubMed] [Google Scholar]
- STEIN W. H., MOORE S. The free amino acids of human blood plasma. J Biol Chem. 1954 Dec;211(2):915–926. [PubMed] [Google Scholar]
- Scharff R., Wool I. G. Accumulation of amino acids in muscle of perfused rat heart. Effect of insulin in the presence of puromycin. Biochem J. 1965 Oct;97(1):272–276. doi: 10.1042/bj0970272. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schwartzman L., Crawhall J., Segal S. Incorporation of amino acids into a lipid fraction of kidney cortex. Biochim Biophys Acta. 1966 Jul 27;124(1):62–70. doi: 10.1016/0304-4165(66)90313-8. [DOI] [PubMed] [Google Scholar]
- Waterlow J. C., Stephen J. M. The effect of low protein diets on the turn-over rates of serums, liver and muscle proteins in the rat, measured by continuous infusion of L-[14C]lysine. Clin Sci. 1968 Oct;35(2):287–305. [PubMed] [Google Scholar]