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. 1969 Sep;114(2):171–178. doi: 10.1042/bj1140171

Relationship between intracellular amino acids and protein synthesis in the extensor digitorum longus muscle of rats

R C Hider 1, E B Fern 1, D R London 1
PMCID: PMC1184841  PMID: 5822066

Abstract

1. The incorporation into protein, and the accumulation into the free amino acid pools, of radioactive l-leucine and glycine was studied in rat extensor digitorum longus muscle. 2. The tissue was incubated first with 14C-labelled and then with 3H-labelled amino acid. 3. The experimental results were consistent with a model based on the premise that the amino acids in protein were incorporated directly from the extracellular pool.

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Selected References

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  1. ASKONAS B. A., HUMPHREY J. H. Formation of specific antibodies and gamma-globulin in vitro; a study of the synthetic ability of various tissues from rabbits immunized by different methods. Biochem J. 1958 Feb;68(2):252–261. doi: 10.1042/bj0680252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CASTLES J. J., WOOL I. G., MOYER A. N. THE EFFECT OF DIABETES ON AMINO ACID ACCUMULATION AND PROTEIN SYNTHESIS IN ISOLATED RAT DIAPHRAGM. Biochim Biophys Acta. 1965 May 4;100:609–612. doi: 10.1016/0304-4165(65)90036-x. [DOI] [PubMed] [Google Scholar]
  3. HENDLER R. W. A model for protein synthesis. Nature. 1962 Mar 3;193:821–823. doi: 10.1038/193821a0. [DOI] [PubMed] [Google Scholar]
  4. HENDLER R. W. ISOLATION OF "LIPOPEPTIDES" FROM HEN OVIDUCT. Biochim Biophys Acta. 1963 Sep 10;74:659–666. doi: 10.1016/0006-3002(63)91417-3. [DOI] [PubMed] [Google Scholar]
  5. Harris E. J., Manchester K. L. The effects of potassium ions and denervation on protein synthesis and the transport of amino acids in muscle. Biochem J. 1966 Oct;101(1):135–145. doi: 10.1042/bj1010135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KIPNIS D. M., CORI C. F. Studies of tissue permeability. III. The effect of insulin on pentose uptake by the diaphragm. J Biol Chem. 1957 Feb;224(2):681–693. [PubMed] [Google Scholar]
  7. KIPNIS D. M., REISS E., HELMREICH E. Functional heterogeneity of the intracellular amino acid pool in mammalian cells. Biochim Biophys Acta. 1961 Aug 19;51:519–524. doi: 10.1016/0006-3002(61)90608-4. [DOI] [PubMed] [Google Scholar]
  8. KOSTYO J. L. SEPARATION OF THE EFFECTS OF GROWTH HORMONE ON MUSCLE AMINO ACID TRANSPORT AND PROTEIN SYNTHESIS. Endocrinology. 1964 Jul;75:113–119. doi: 10.1210/endo-75-1-113. [DOI] [PubMed] [Google Scholar]
  9. KRAHL M. E. STIMULATION OF PEPTIDE SYNTHESIS IN ADIPOSE TISSUE BY INSULIN WITHOUT GLUCOSE. Am J Physiol. 1964 Mar;206:618–620. doi: 10.1152/ajplegacy.1964.206.3.618. [DOI] [PubMed] [Google Scholar]
  10. MANCHESTER K. L. Insulin and incorporation of amino acids into protein of muscle. Cellular amino acid levels and aminoisobutyric acid uptake. Biochem J. 1961 Oct;81:135–147. doi: 10.1042/bj0810135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MANCHESTER K. L., WOOL I. G. INSULIN AND INCORPORATION OF AMINO ACIDS INTO PROTEIN OF MUSCLE. 1. ACCUMULATION AND INCORPORATION STUDIES WITH THE PERFUSED RAT HEART. Biochem J. 1963 Nov;89:202–209. doi: 10.1042/bj0890202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Manchester K. L. Some factors affecting the response of muscle to insulin. Biochem J. 1966 Mar;98(3):711–719. doi: 10.1042/bj0980711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. OXENDER D. L., CHRISTENSEN H. N. DISTINCT MEDIATING SYSTEMS FOR THE TRANSPORT OF NEUTRAL AMINO ACIDS BY THE EHRLICH CELL. J Biol Chem. 1963 Nov;238:3686–3699. [PubMed] [Google Scholar]
  14. RIGGS T. R., WALKER L. M. SOME RELATIONS BETWEEN ACTIVE TRANSPORT OF FREE AMINO ACIDS INTO CELLS AND THEIR INCORPORATION INTO PROTEIN. J Biol Chem. 1963 Aug;238:2663–2668. [PubMed] [Google Scholar]
  15. ROBERTS L. E., BENSCH K., CARTER C. E. AMINO ACID-ACTIVATED ADENOSINE TRIPHOSPHATE-PYROPHOSPHATE EXCHANGE BY MODIFIED ESCHERICHIA COLI CELLS. Biochim Biophys Acta. 1964 Aug 19;90:291–300. doi: 10.1016/0304-4165(64)90192-8. [DOI] [PubMed] [Google Scholar]
  16. ROSENBERG L. E., BERMAN M., SEGAL S. Studies of the kinetics of amino acid transport, incorporation into portein and oxidation in kidney-cortex slices. Biochim Biophys Acta. 1963 Jun 4;71:664–675. doi: 10.1016/0006-3002(63)91140-5. [DOI] [PubMed] [Google Scholar]
  17. Riggs T. R., Pan M. W., Feng H. W. Transport of amino acids into the estrogen-primed uterus. I. General characteristics of the uptake in vitro. Biochim Biophys Acta. 1968 Jan 3;150(1):92–103. doi: 10.1016/0005-2736(68)90012-6. [DOI] [PubMed] [Google Scholar]
  18. Roscoe J. P., Eaton M. D., Choy G. C. Inhibition of protein synthesis in Krebs 2 ascites cells and cell-free systems by phenylalanine and its effect on leucine and lysine in the amino acid pool. Biochem J. 1968 Oct;109(4):507–515. doi: 10.1042/bj1090507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SEGAL S. HORMONES, AMINO-ACID TRANSPORT AND PROTEIN SYNTHESIS. Nature. 1964 Jul 4;203:17–19. doi: 10.1038/203017a0. [DOI] [PubMed] [Google Scholar]
  20. STEIN W. H., MOORE S. The free amino acids of human blood plasma. J Biol Chem. 1954 Dec;211(2):915–926. [PubMed] [Google Scholar]
  21. Scharff R., Wool I. G. Accumulation of amino acids in muscle of perfused rat heart. Effect of insulin in the presence of puromycin. Biochem J. 1965 Oct;97(1):272–276. doi: 10.1042/bj0970272. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schwartzman L., Crawhall J., Segal S. Incorporation of amino acids into a lipid fraction of kidney cortex. Biochim Biophys Acta. 1966 Jul 27;124(1):62–70. doi: 10.1016/0304-4165(66)90313-8. [DOI] [PubMed] [Google Scholar]
  23. Waterlow J. C., Stephen J. M. The effect of low protein diets on the turn-over rates of serums, liver and muscle proteins in the rat, measured by continuous infusion of L-[14C]lysine. Clin Sci. 1968 Oct;35(2):287–305. [PubMed] [Google Scholar]

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