Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1969 Sep;114(2):299–305. doi: 10.1042/bj1140299

A rat liver system that catalyses a pyridoxal phosphate-independent αβ-elimination

N Tudball 1, J H Thomas 1,*, J A Fowler 1,
PMCID: PMC1184855  PMID: 5810088

Abstract

1. Attempts were made to demonstrate the presence of pyridoxal phosphate in the rat liver system catalysing the αβ-elimination of l-serine O-sulphate. 2. Methods designed to resolve protein-bound cofactor, spectroscopic examination of a purified enzyme system and attempted reactivation of apo-(alanine aminotransferase) failed to demonstrate the presence of pyridoxal phosphate. 3. The activity of the αβ-eliminating system remained constant in vitamin B6-deficient animals even though the activities of other pyridoxal phosphate-dependent systems fell markedly. 4. The metabolism of l-serine O[35S]-sulphate in vivo appears to be normal in vitamin B6-deficient animals. 5. No incorporation of tritium into the αβ-eliminating system occurred after administration of tritiated pyridoxine to experimental animals.

Full text

PDF
299

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLAKLEY R. L. The interconversion of serine and glycine: participation of pyridoxal phosphate. Biochem J. 1955 Oct;61(2):315–323. doi: 10.1042/bj0610315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BLASCHKO H., BUFFONI F. PYRIDOXAL PHOSPHATE AS A CONSTITUENT OF THE HISTAMINASE (BENZYLAMINE OXIDASE) OF PIG PLASMA. Proc R Soc Lond B Biol Sci. 1965 Aug 24;163:45–60. doi: 10.1098/rspb.1965.0059. [DOI] [PubMed] [Google Scholar]
  3. BRIN M., OLSON R. E., STARE F. J. Metabolism of cardiac muscle. VIII. Pyridoxine deficiency. J Biol Chem. 1954 Sep;210(1):435–444. [PubMed] [Google Scholar]
  4. Brown F. C., Mallady B. J., Roszell J. A. The separation of rat liver serine dehydratase and cystathionine synthase. J Biol Chem. 1966 Nov 25;241(22):5220–5224. [PubMed] [Google Scholar]
  5. CALDWELL E. F., MCHENRY E. W. Studies on vitamin B6 and transamination in rat liver. Arch Biochem Biophys. 1953 Jul;45(1):97–104. doi: 10.1016/0003-9861(53)90409-3. [DOI] [PubMed] [Google Scholar]
  6. Cori C. F., Illingworth B. THE PROSTHETIC GROUP OF PHOSPHORYLASE. Proc Natl Acad Sci U S A. 1957 Jul 15;43(7):547–552. doi: 10.1073/pnas.43.7.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dupourque D., Newton W. A., Snell E. E. Purification and properties of D-serine dehydrase from Escherichia coli. J Biol Chem. 1966 Mar 10;241(5):1233–1238. [PubMed] [Google Scholar]
  8. GREGERMAN R. I., CHRISTENSEN H. N. Enzymatic and non-enzymatic dehydrochlorination of beta-chloro-L-alanine. J Biol Chem. 1956 Jun;220(2):765–774. [PubMed] [Google Scholar]
  9. JENKINS W. T., SIZER I. W. Glutamic aspartic transaminase. II. The influence of pH on absorption spectrum and enzymatic activity. J Biol Chem. 1959 May;234(5):1179–1181. [PubMed] [Google Scholar]
  10. Labow R., Robinson W. G. Crystalline D-serine dehydrase. J Biol Chem. 1966 Mar 10;241(5):1239–1243. [PubMed] [Google Scholar]
  11. MARTINEZ-CARRION M., JENKINS W. T. SOME PROPERTIES OF A D-ALANINE D-GLUTAMATE AMINOTRANSFERASE PURIFIED FROM BACILLUS SUBTILIS. Biochem Biophys Res Commun. 1963 Aug 14;12:365–368. doi: 10.1016/0006-291x(63)90106-2. [DOI] [PubMed] [Google Scholar]
  12. MATSUO Y., GREENBERG D. M. A crystalline enzyme that cleaves homoserine and cystathionine. II. Prosthetic group. J Biol Chem. 1958 Feb;230(2):561–571. [PubMed] [Google Scholar]
  13. MATSUO Y., GREENBERG D. M. A crystalline enzyme that cleaves homoserine and cystathionine. III. Coenzyme resolution, activators, and inhibitors. J Biol Chem. 1959 Mar;234(3):507–515. [PubMed] [Google Scholar]
  14. Mondovì B., Costa M. T., Agrò A. F., Rotilio G. Pyridoxal phosphate as a prosthetic group of pig kidney diamine oxidase. Arch Biochem Biophys. 1967 Mar;119(1):373–381. doi: 10.1016/0003-9861(67)90468-7. [DOI] [PubMed] [Google Scholar]
  15. NAGABHUSHANAM A., GREENBERG D. M. ISOLATION AND PROPERTIES OF A HOMOGENEOUS PREPARATION OF CYSTATHIONINE SYNTHETASE-L-SERINE AND L-THREONINE DEHYDRATASE. J Biol Chem. 1965 Jul;240:3002–3008. [PubMed] [Google Scholar]
  16. NISHIMURA J. S., GREENBERG D. M. Purification and properties of L-threonine dehydrase of sheep liver. J Biol Chem. 1961 Oct;236:2684–2691. [PubMed] [Google Scholar]
  17. Radhakrishnamurty R., Sabry Z. I. Liver and erythrocyte L-aspartate: and L-alanine: 2-oxoglutarate aminotransferases of the pyridoxine-deficient rat. Can J Biochem. 1968 Sep;46(9):1081–1088. doi: 10.1139/o68-161. [DOI] [PubMed] [Google Scholar]
  18. Riley W. D., Snell E. E. Histidine decarboxylase of Lactobacillus 30a. IV. The presence of covalently bound pyruvate as the prosthetic group. Biochemistry. 1968 Oct;7(10):3520–3528. doi: 10.1021/bi00850a029. [DOI] [PubMed] [Google Scholar]
  19. SELIM A. S., GREENBERG D. M. An enzyme that synthesizes cystathionine and deaminates L-serine. J Biol Chem. 1959 Jun;234(6):1474–1480. [PubMed] [Google Scholar]
  20. SHUKUYA R., SCHWERT G. W. Glutamic acid decarboxylase. II. The spectrum of the enzyme. J Biol Chem. 1960 Jun;235:1653–1657. [PubMed] [Google Scholar]
  21. Stielau W. J., Freedland R. A., Meyer J. H. Effects of B-vitamin deficiencies and of starvation on liver enzyme activities of growing rats. J Nutr. 1965 Sep;87(1):109–116. doi: 10.1093/jn/87.1.109. [DOI] [PubMed] [Google Scholar]
  22. TONHAZY N. E., WHITE N. G., UMBREIT W. W. A rapid method for the estimation of the glutamic-aspartic transaminase in tissues and its application to radiation sickness. Arch Biochem. 1950 Aug;28(1):36–42. [PubMed] [Google Scholar]
  23. TUDBALL N., NODA Y., DODGSON K. S. THE METABOLISM OF L-SERYLGLYCINE O(35S)-SULPHATE IN THE RAT. Biochem J. 1965 Jun;95:678–682. doi: 10.1042/bj0950678. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Thomas J. H., Dodgson K. S., Tudball N. The pyridoxal- and pyridoxal 5'-phosphate-catalysed non-enzymic degradations of l-serine o-sulphate and related compounds. Biochem J. 1968 Dec;110(4):687–692. doi: 10.1042/bj1100687. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Thomas J. H., Tudball N. Studies on the enzymic degradation of L-serine O-sulphate by a rat liver preparation. Biochem J. 1967 Nov;105(2):467–472. doi: 10.1042/bj1050467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tudball N. The metabolism of potassium l-serine O[S]-sulphate in the rat. Biochem J. 1962 Dec;85(3):456–460. doi: 10.1042/bj0850456. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. WISS O., WEBER F. BIOCHEMICAL PATHOLOGY OF VITAMIN B6 DEFICIENCY. Vitam Horm. 1964;22:495–501. doi: 10.1016/s0083-6729(08)60350-7. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES