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. 1969 Sep;114(3):455–461. doi: 10.1042/bj1140455

Intramitochondrial localization of δ-aminolaevulate synthetase and ferrochelatase in rat liver

Roxane McKay 1, R Druyan 1, G S Getz 1, M Rabinowitz 1
PMCID: PMC1184917  PMID: 5820634

Abstract

Intramitochondrial loci for δ-aminolaevulate synthetase and ferrochelatase, the initial and final enzymes in haem synthesis, have been found in rat liver. Two different methods of fractionation were applied to mitochondria: (a) sonication and density-gradient centrifugation; (b) treatment with digitonin and differential centrifugation. Similar results were obtained with each technique. δ-Aminolaevulate synthetase is distributed similarly to two known matrix enzymes, malate dehydrogenase and glutamate dehydrogenase. Ferrochelatase is firmly bound to the the inner mitochondrial membrane. These results are considered in terms of the regulation of haem synthesis and in relation to mitochondrial biogenesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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