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. 1969 Sep;114(3):529–534. doi: 10.1042/bj1140529

Substituent effect on lysozyme-catalysed hydrolysis of some β-aryl di-N-acetylchitobiosides

C S Tsai 1, J Y Tang 1, S C Subbarao 1
PMCID: PMC1184925  PMID: 5820639

Abstract

Measurements are reported on the kinetics of the lysozyme-catalysed hydrolysis of several β-aryl di-N-acetylchitobiosides, some of which have been synthesized for the first time. The catalytic rate constants (kcat.) at 45° yield a curved Hammett plot (concave up) and the plot of ΔH‡ versus ΔS‡ has a sharp break. Substrates with electron-withdrawing groups exhibit a kinetic deuterium isotope effect (kHcat./kDcat.), whereas those with electron-donating groups show no such isotope effect. The results suggest the operation of different mechanisms for the two types of substrates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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