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. 1969 Nov;115(3):583–586. doi: 10.1042/bj1150583

Studies on the metabolism of steroids in the foetus. Biosynthesis of 6α-hydroxytestosterone in the human foetal liver

B P Lisboa 1, J-Å Gustafsson 1
PMCID: PMC1185141  PMID: 5353530

Abstract

After incubation of testosterone with 105000g microsomes of human foetal liver, 6α-hydroxytestosterone was isolated and identified by t.l.c. and g.l.c.–mass spectrometry. This is the first example of 6α-hydroxylation of C19 steroids in the human liver, and the finding is discussed in relation to earlier reports of 6-oxygenated C19 and C18 steroids in pregnant women.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alonso C., Troen P. Perfusion studies of the human placenta. V. Metabolism of [3H)estrone [35S]sulfate and [14C]estradiol-17-beta at mid-pregnancy. Biochemistry. 1966 Jan;5(1):337–343. doi: 10.1021/bi00865a043. [DOI] [PubMed] [Google Scholar]
  2. BREUER H., KNUPPEN R., ORTLEPP R., PANGELS G., PUCK A. Biogenesis of 6-hydroxylated oestrogens in human tissues. Biochim Biophys Acta. 1960 Jun 3;40:560–561. doi: 10.1016/0006-3002(60)91408-6. [DOI] [PubMed] [Google Scholar]
  3. FRANTZ A. G., KATZ F. H., JAILER J. W. 6beta-Hydroxycortisol and other polar corticosteroids: measurement and significance in human urine. J Clin Endocrinol Metab. 1961 Oct;21:1290–1303. doi: 10.1210/jcem-21-10-1290. [DOI] [PubMed] [Google Scholar]
  4. Gardiner W. L., Herning E. C. Gas-liquid chromatographic separation of C19 and C21 human urinary steroids by a new procedure. Biochim Biophys Acta. 1966 Feb 28;115(2):524–526. doi: 10.1016/0304-4165(66)90461-2. [DOI] [PubMed] [Google Scholar]
  5. Gustafsson J. A., Lisboa B. P. Biosynthesis of 6-beta-hydroxytestosterone from testosterone by human fetal iver microsomes. Steroids. 1968 Apr;11(4):555–563. doi: 10.1016/s0039-128x(68)80068-6. [DOI] [PubMed] [Google Scholar]
  6. Horning M. G., Moss A. M., Horning E. C. Formation and gas-liquid chromatographic behavior of isometric steroid ketone methoxime derivatives. Anal Biochem. 1968 Feb;22(2):284–294. doi: 10.1016/0003-2697(68)90318-7. [DOI] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Lisboa B. P., Gustafsson J. A. Biosynthesis of 18-hydroxytestosterone in the human foetal liver. Eur J Biochem. 1969 Jun;9(3):402–405. doi: 10.1111/j.1432-1033.1969.tb00622.x. [DOI] [PubMed] [Google Scholar]
  9. Lisboa B. P., Gustafsson J. A. Biosynthesis of 6 beta- and 6 alpha-hydroxyprogesterone in the human foetal liver. Eur J Biochem. 1969 Jul;9(4):503–506. doi: 10.1111/j.1432-1033.1969.tb00637.x. [DOI] [PubMed] [Google Scholar]
  10. Lisboa B. P., Gustafsson J. A. Biosynthesis of two new steroids in the human foetal liver, 1 beta- and 2 beta-hydroxytestosterone. Eur J Biochem. 1968 Nov;6(3):419–424. doi: 10.1111/j.1432-1033.1968.tb00463.x. [DOI] [PubMed] [Google Scholar]
  11. MAKITA M., WELLS W. W. Quantitative analysis of fecal bile acids by gas-liquid chromatography. Anal Biochem. 1963 Jun;5:523–530. doi: 10.1016/0003-2697(63)90072-1. [DOI] [PubMed] [Google Scholar]

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