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. 1969 Dec;115(4):663–669. doi: 10.1042/bj1150663

Investigations on metabolites of vitamin D in rat bile. Separation and partial identification of a major metabolite

P A Bell 1, E Kodicek 1
PMCID: PMC1185191  PMID: 4311438

Abstract

1. Young rats with cannulated bile ducts were given 0·34mg. of [1α-3H]cholecalciferol or 0·54mg. of [14C]ergocalciferol by intravenous infusion. Of the radioactivity in the dose of [1α-3H]cholecalciferol 31% was recovered in bile within 24hr. 2. The metabolites in bile were separated by gradient-elution column chromatography on silicic acid into five components, all more polar than cholecalciferol or 25-hydroxycholecalciferol. [14C]Ergocalciferol gave a similar pattern of metabolites in bile. 3. The three most polar metabolites were shown to be ionic. The major component has been identified as a glucuronide conjugate, which was not identical with synthetic cholecalciferyl glucuronide.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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