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. 1969 Dec;115(5):1005–1007. doi: 10.1042/bj1151005

Studies on the conversion of 60s yeast ribosomes into a 50s component

Nandini Ghosh 1
PMCID: PMC1185243  PMID: 5360672

Abstract

The process of conversion of the larger (60s) subunit of yeast ribosomes into a 50s component was studied. The release of any RNA or protein material during conversion was assayed by using 32P- or 35S-labelled ribosomes; ribosomal RNA distributions of the particles were examined and protein/RNA ratios of subunits were determined. The change in sedimentation coefficients was found to be due, not to loss of material, but to a structural change. The change in structure was shown by electron microscopy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHAO F. C. Dissociation of macromolecular ribonucleoprotein of yeast. Arch Biochem Biophys. 1957 Aug;70(2):426–431. doi: 10.1016/0003-9861(57)90130-3. [DOI] [PubMed] [Google Scholar]
  2. ELSON D. A ribonucleic acid particle released from ribosomes by salt. Biochim Biophys Acta. 1961 Oct 14;53:232–234. doi: 10.1016/0006-3002(61)90818-6. [DOI] [PubMed] [Google Scholar]
  3. Gavrilova L. P., Ivanov D. A., Spirin A. S. Studies on the structure of ribosomes. 3. Stepwise unfolding of the 50 s particles without loss of ribosomal protein. J Mol Biol. 1966 Apr;16(2):473–489. doi: 10.1016/s0022-2836(66)80186-9. [DOI] [PubMed] [Google Scholar]
  4. Gesteland R. F. Unfolding of Escherichia coli ribosomes by removal of magnesium. J Mol Biol. 1966 Jul;18(2):356–371. doi: 10.1016/s0022-2836(66)80253-x. [DOI] [PubMed] [Google Scholar]
  5. HALL C. E. Measurement of globular protein molecules by electron microscopy. J Biophys Biochem Cytol. 1960 Jul;7:613–618. doi: 10.1083/jcb.7.4.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Miall S. H., Walker I. O. Structural studies on ribosomes. II. Denaturation and sedimentation of ribosomal subunits unfolded in EDTA. Biochim Biophys Acta. 1969 Feb 18;174(2):551–560. doi: 10.1016/0005-2787(69)90284-6. [DOI] [PubMed] [Google Scholar]
  8. Natori S., Maruta H., Mizuno D. Unfolding of Escherichia coli ribosomes by phosphate ion in the presence of oligonucleotides. J Mol Biol. 1968 Nov 28;38(1):109–119. doi: 10.1016/0022-2836(68)90131-9. [DOI] [PubMed] [Google Scholar]

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