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. 1970 Jan;116(2):171–175. doi: 10.1042/bj1160171

The thermodynamics of interaction between Sephadex and penetrating solutes

A G Ogston 1, Panee Silpananta 1,*
PMCID: PMC1185342  PMID: 4905851

Abstract

1. We have measured the partition coefficients of bovine serum albumin with Sephadex grades G-100, G-150 and G-200, and of a dextran ([unk]n 19700) and a polyethylene glycol ([unk]n 8000) with Sephadex G-200. We have also measured the effects of these solutes on the inner volumes of the grades of Sephadex. 2. The results can be described with fair consistency by means of a simple thermodynamic treatment that makes use of the virial coefficients of Sephadex and of the solute, and of a coefficient that expresses their interaction. This coefficient is related to the `exclusion volume' of Sephadex for the solutes. 3. The Sephadex G-200–polyethylene glycol system shows anomalies of behaviour that are ascribed to the occurrence of `incompatible' phase separation within the Sephadex beads.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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