Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1970 Jan;116(2):199–205. doi: 10.1042/bj1160199

The fractionation of dinucleoside monophosphate and some trinucleoside diphosphate isonicotinoyl hydrazones by column chromatography

John A Hunt 1
PMCID: PMC1185346  PMID: 5414095

Abstract

A column-chromatographic system using DEAE-cellulose and gradient elution with triethylammonium formate at pH4.0–3.5 is described. It is capable of separating the oligonucleotide isonicotinoyl hydrazones that are produced by nuclease digestion of RNA oxidized with periodate and coupled with isonicotinic acid hydrazide. Fifteen dinucleoside monophosphate isonicotinoyl hydrazones were characterized by their elution positions on the columns, so that all but two of them could readily be identified. Twelve trinucleoside diphosphate hydrazones were also characterized by their elution positions on the column. The application of this method of fractionation to terminal-sequence studies of RNA is discussed.

Full text

PDF
199

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. HUNT J. A. TERMINAL-SEQUENCE STUDIES OF HIGH-MOLECULAR-WEIGHT RIBONUCLEIC. THE REACTION OF PERIODATE-OXIDIZED RIBONUCLEOSIDES , 5'-RIBONUCLEOTIDES AND RIBONUCLEIC ACID WITH ISONIAZID. Biochem J. 1965 May;95:541–551. doi: 10.1042/bj0950541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hunt J. A. Continuous-flow monitor system for detection of UV absorbance, 14C, and 3H in effluent of a column chromatogram. Anal Biochem. 1968 May;23(2):289–300. doi: 10.1016/0003-2697(68)90360-6. [DOI] [PubMed] [Google Scholar]
  3. Midgley J. E. The isonicotinyl hydrazones of nucleosides and their phosphorylated derivatives. Biochim Biophys Acta. 1966 Jul 20;123(1):210–213. doi: 10.1016/0005-2787(66)90177-8. [DOI] [PubMed] [Google Scholar]
  4. Steinschneider A., Fraenkel-Conrat H. Studies of nucleotide sequences in tobacco mosaic virus ribonucleic acid. IV. Use of aniline in stepwise degradation. Biochemistry. 1966 Aug;5(8):2735–2743. doi: 10.1021/bi00872a034. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES