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. 1978 Apr 15;172(1):181–184. doi: 10.1042/bj1720181

Loss of cell-population-density-dependent incorporation of fucose into the lipid fraction of cultured human tumour cells.

M Gacto
PMCID: PMC1185677  PMID: 656070

Abstract

The incorporation of radioactively labelled fucose into the lipid fraction of cultured normal human cells and several human tumour-cell lines was examined as a function of the cell population density. Normal cells exhibited a density-dependent pattern of incorporation, whereas in tumour cells the radioactivity incorporated was independent of the cell population density. An exception was found among the tumour cells, which suggests a possible correlation between the loss of this marker and the ability to produce tumours.

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Selected References

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  1. Arnstein P., Taylor D. O., Nelson-Rees W. A., Huebner R. J., Lennette E. H. Propagation of human tumors in antithymocyte serum-treated mice. J Natl Cancer Inst. 1974 Jan;52(1):71–84. doi: 10.1093/jnci/52.1.71. [DOI] [PubMed] [Google Scholar]
  2. Critchley D. R., Macpherson I. Cell density dependent glycolipids in NILz hamster cells, derived malignant and transformed cell lines. Biochim Biophys Acta. 1973 Jan 19;296(1):145–159. doi: 10.1016/0005-2760(73)90054-4. [DOI] [PubMed] [Google Scholar]
  3. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  4. Gacto M., Steiner S. Fucolipid metabolism as a function of cell population density in normal and murine sarcoma virus-transformed rat cells. Biochim Biophys Acta. 1976 Aug 24;444(1):11–22. doi: 10.1016/0304-4165(76)90219-1. [DOI] [PubMed] [Google Scholar]
  5. Giard D. J., Aaronson S. A., Todaro G. J., Arnstein P., Kersey J. H., Dosik H., Parks W. P. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J Natl Cancer Inst. 1973 Nov;51(5):1417–1423. doi: 10.1093/jnci/51.5.1417. [DOI] [PubMed] [Google Scholar]
  6. Hakomori S. Cell density-dependent changes of glycolipid concentrations in fibroblasts, and loss of this response in virus-transformed cells. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1741–1747. doi: 10.1073/pnas.67.4.1741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hakomori S. Structures and organization of cell surface glycolipids dependency on cell growth and malignant transformation. Biochim Biophys Acta. 1975 Mar 20;417(1):55–89. doi: 10.1016/0304-419x(75)90008-6. [DOI] [PubMed] [Google Scholar]
  8. Hynes R. O., Bye J. M. Density and cell cycle dependence of cell surface proteins in hamster fibroblasts. Cell. 1974 Oct;3(2):113–120. doi: 10.1016/0092-8674(74)90114-7. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Larriba G., Klinger M., Sramek S., Steiner S. Noval fucose-containing components from rat tissues. Biochem Biophys Res Commun. 1977 Jul 11;77(1):79–85. doi: 10.1016/s0006-291x(77)80167-8. [DOI] [PubMed] [Google Scholar]
  11. Richardson C. L., Baker S. R., Morré D. J., Keenan T. W. Glycosphingolipid synthesis and tumorigenesis. A role for the Golgi apparatus in the origin of specific receptor molecules of the mammalian cell surface. Biochim Biophys Acta. 1975 Dec 31;417(3-4):175–186. doi: 10.1016/0304-419x(75)90009-8. [DOI] [PubMed] [Google Scholar]
  12. Robbins P. W., Macpherson I. Control of glycolipid synthesis in a cultured hamster cell line. Nature. 1971 Feb 19;229(5286):569–570. doi: 10.1038/229569a0. [DOI] [PubMed] [Google Scholar]
  13. Roseman S. The synthesis of complex carbohydrates by multiglycosyltransferase systems and their potential function in intercellular adhesion. Chem Phys Lipids. 1970 Oct;5(1):270–297. doi: 10.1016/0009-3084(70)90024-1. [DOI] [PubMed] [Google Scholar]
  14. Sakiyama H., Robbins P. W. Glycolipid synthesis and tumorigenicity of clones isolated from the Nil 2 line of hamster embryo fibroblasts. Fed Proc. 1973 Jan;32(1):86–90. [PubMed] [Google Scholar]
  15. Sakiyama H., Terasima T. The synthesis of Forssman glycolipid in clones of nil 2 hamsters fibroblasts grown in monolayer or spinner culture. Cancer Res. 1975 Jul;35(7):1723–1726. [PubMed] [Google Scholar]
  16. Steiner S., Melnick J. L. Altered fucolipid patterns in cultured human cancer cells. Nature. 1974 Oct 25;251(5477):717–718. doi: 10.1038/251717a0. [DOI] [PubMed] [Google Scholar]
  17. Yogeeswaran G., Hakomori S. Cell contact-dependent ganglioside changes in mouse 3T3 gibroblasts and a suppressed sialidase activity on cell contact. Biochemistry. 1975 May 20;14(10):2151–2156. doi: 10.1021/bi00681a017. [DOI] [PubMed] [Google Scholar]
  18. Yogeeswaran G., Laine R. A., Hakomori S. Mechanism of cell contact-dependent glycolipid synthesis: further studies with glycolipid-glass complex. Biochem Biophys Res Commun. 1974 Jul 24;59(2):591–599. doi: 10.1016/s0006-291x(74)80021-5. [DOI] [PubMed] [Google Scholar]

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