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. 1978 Apr 15;172(1):189–192. doi: 10.1042/bj1720189

Fractionation of microsomal membranes on the basis of their surface properties.

R Ohlsson, B Jergil, H Walter
PMCID: PMC1185679  PMID: 656072

Abstract

Partition in dextran-poly(ethylene glycol) aqueous-phase systems can be used for both membrane subfractionation and gaining information on membrane surface properties [H. Walter (1977) in Methods of Cell Separation (Catsimpoolas, N., ed.), vol. 1, pp. 307-354, Plenum, New York]. Smooth, light rough and heavy rough rat liver microsome (obtained by sucrose-density-gradient centrifugation) were subjected to countercurrent distribution in such a system. Smooth microsomal membranes had the highest, heavy rough microsomal membranes the lowest and light rough microsomal membranes an intermediate partition coefficient. The separation is based primarily on hydrophobic differences in the membrane surfaces of the three preparations and is thus due to microsomal properties not previously utilized in their fractionation. The method permits additional subfractionations of microsomes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blad D., Winqvist L., Dallner G. Electrophoretic mobility of microsomes from rat liver. J Cell Sci. 1977 Feb;23:285–297. doi: 10.1242/jcs.23.1.285. [DOI] [PubMed] [Google Scholar]
  2. Borochov H., Zahler P., Wilbrandt W., Shinitzky M. The effect of phosphatidylcholine to sphingomyelin mole ratio on the dynamic properties of sheep erythrocyte membrane. Biochim Biophys Acta. 1977 Nov 1;470(3):382–388. doi: 10.1016/0005-2736(77)90129-8. [DOI] [PubMed] [Google Scholar]
  3. Brooks D. E., Seaman G. V., Walter H. Detection of differences in surface-charge-associated properties of cells by partition in two-polymer aqueous phase systems. Nat New Biol. 1971 Nov 10;234(45):61–62. doi: 10.1038/newbio234061a0. [DOI] [PubMed] [Google Scholar]
  4. Depierre J. W., Dallner G. Structural aspects of the membrane of the endoplasmic reticulum. Biochim Biophys Acta. 1975 Dec 29;415(4):411–472. doi: 10.1016/0304-4157(75)90006-4. [DOI] [PubMed] [Google Scholar]
  5. FLECK A., MUNRO H. N. The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation. Biochim Biophys Acta. 1962 May 14;55:571–583. doi: 10.1016/0006-3002(62)90836-3. [DOI] [PubMed] [Google Scholar]
  6. Higgins J. A., Dawson R. M. Asymmetry of the phospholipid bilayer of rat liver endoplasmic reticulum. Biochim Biophys Acta. 1977 Nov 1;470(3):342–356. doi: 10.1016/0005-2736(77)90126-2. [DOI] [PubMed] [Google Scholar]
  7. Johansson G. Partition of salts and their effects on partition of proteins in a dextran-poly (ethylene glycol)-water two-phase system. Biochim Biophys Acta. 1970 Nov 17;221(2):387–390. doi: 10.1016/0005-2795(70)90283-7. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Lewis J. A., Tata J. R. Heterogeneous distribution of glucose 6-phosphatase in rat liver microsomal fractions as shown by adaptation of a cytochemical technique. Biochem J. 1973 May;134(1):69–78. doi: 10.1042/bj1340069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ohlsson R., Jergil B. On the involvement of cytochrome P-450 in the binding of ribosomes to a subfraction of rat-liver rapidly sedimenting endoplasmic reticulum. Eur J Biochem. 1977 Feb;72(3):595–603. doi: 10.1111/j.1432-1033.1977.tb11282.x. [DOI] [PubMed] [Google Scholar]
  11. Reitherman R., Flanagan S. D., Barondes S. H. Electromotive phenomena in partition of erythrocytes in aqueous polymer two phase systems. Biochim Biophys Acta. 1973 Feb 28;297(2):193–202. doi: 10.1016/0304-4165(73)90065-2. [DOI] [PubMed] [Google Scholar]
  12. Schulze H. U., Pönnighaus J. M., Staudinger H. Untersuchungen über die Verteilung von Enzymproteinen in den endoplasmatischen Membranen der Leberzelle. II. Immunologische Fraktionierung von Schweinelebermikrosomen. Hoppe Seylers Z Physiol Chem. 1972 Aug;353(8):1195–1204. [PubMed] [Google Scholar]
  13. Sundler R., Sarcione S. L., Alberts A. W., Vagelos P. R. Evidence against phospholipid asymmetry in intracellular membranes from liver. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3350–3354. doi: 10.1073/pnas.74.8.3350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Walter H., Krob E. J., Brooks D. E. Membrane surface properties other than charge involved in cell separation by partition in polymer, aqueous two-phase systems. Biochemistry. 1976 Jul 13;15(14):2959–2964. doi: 10.1021/bi00659a004. [DOI] [PubMed] [Google Scholar]
  15. Walter H., Krob E. J. Partition in two-polymer aqueous phases reflects differences between membrane surface properties of erythrocytes, ghosts and membrane vesicles. Biochim Biophys Acta. 1976 Nov 11;455(1):8–23. doi: 10.1016/0005-2736(76)90150-4. [DOI] [PubMed] [Google Scholar]

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