Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1978 May 15;172(2):285–291. doi: 10.1042/bj1720285

A radiochemical assay for glycine N-acyltransferase activity. Some properties of the enzyme in rat and rabbit.

M O James, J R Bend
PMCID: PMC1185694  PMID: 666745

Abstract

We have developed a sensitive radiochemical assay of glycine N-acyltransferase activity, using phenylacetyl-CoA as the acyl donor and glycine as the acceptor. This assay measures formation of the product, phenylacetylglycine, instead of disappearance of the substrate, phenylacetyl-CoA, as did earlier assays. The subcellular location and some properties of the conjugating activity were determined in liver and kidney of the rabbit and the rat. Rabbit lung and intestine were also tested for activity.

Full text

PDF
285

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amsel L. P., Levy G. Drug biotransformation interactions in man. II. A pharmacokinetic study of the simultaneous conjugation of benzoic and salicylic acids with glycine. J Pharm Sci. 1969 Mar;58(3):321–326. doi: 10.1002/jps.2600580307. [DOI] [PubMed] [Google Scholar]
  2. Andreae W. A., Good N. E. Studies on 3-Indoleacetic Acid Metabolism. IV. Conjugation with Aspartic Acid and Ammonia as Processes in the Metabolism of Carboxylic Acids. Plant Physiol. 1957 Nov;32(6):566–572. doi: 10.1104/pp.32.6.566. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brandt I. K., Simmons P., Gutfinger T. Rat liver glycine acyltransferase: partial purification and some properties. Biochim Biophys Acta. 1968 Aug 27;167(1):196–198. doi: 10.1016/0005-2744(68)90293-3. [DOI] [PubMed] [Google Scholar]
  4. CLELAND W. W. Computer programmes for processing enzyme kinetic data. Nature. 1963 May 4;198:463–465. doi: 10.1038/198463a0. [DOI] [PubMed] [Google Scholar]
  5. DE DUVE C., PRESSMAN B. C., GIANETTO R., WATTIAUX R., APPELMANS F. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue. Biochem J. 1955 Aug;60(4):604–617. doi: 10.1042/bj0600604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dixon P. A., Caldwell J., Woods C. J., Smith R. L. Influence of chemical structure on the pattern of conjugation in vivo and in vitro of some arylacetic acids in the rat. Biochem Soc Trans. 1976;4(1):143–145. doi: 10.1042/bst0040143. [DOI] [PubMed] [Google Scholar]
  7. Fabacher D. L., Hodgson E. Induction of hepatic mixed-function oxidase enzymes in adult and neonatal mice by kepone and mirex. Toxicol Appl Pharmacol. 1976 Oct;38(1):71–77. doi: 10.1016/0041-008x(76)90161-7. [DOI] [PubMed] [Google Scholar]
  8. Forman W. B., Davidson E. D., Webster L. T., Jr Enzymatic conversion of salicylate to salicylurate. Mol Pharmacol. 1971 May;7(3):247–259. [PubMed] [Google Scholar]
  9. Fouts J. R., Devereux T. R. Use of 10-sec sonication of homogenates to increase microsomal protein yield in liver and lung from young and adult Dutch Belt rabbits. Biochem Pharmacol. 1973 Jun 1;22(11):1393–1396. doi: 10.1016/0006-2952(73)90315-8. [DOI] [PubMed] [Google Scholar]
  10. Howland R. D., Bohm L. D. Possible multiple binding sites for o-aminophenol on uridine diphosphate glucuronyltransferase. Biochem J. 1977 Apr 1;163(1):125–131. doi: 10.1042/bj1630125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. James M. O., Smith R. L., Williams R. T., Reidenberg M. The conjugation of phenylacetic acid in man, sub-human primates and some non-primate species. Proc R Soc Lond B Biol Sci. 1972 Jul 25;182(1066):25–35. doi: 10.1098/rspb.1972.0064. [DOI] [PubMed] [Google Scholar]
  12. KIELLEY R. K., SCHNEIDER W. C. Synthesis of p-aminohippuric acid by mitochondria of mouse liver homogenates. J Biol Chem. 1950 Aug;185(2):869–880. [PubMed] [Google Scholar]
  13. Kalina M., Weavers B., Pearse A. G. Ultrastructural localization of succinate dehydrogenase in mouse liver mitochondria; a cytochemical study. J Histochem Cytochem. 1971 Feb;19(2):124–130. doi: 10.1177/19.2.124. [DOI] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. MOLDAVE K., MEISTER A. Synthesis of phenylacetylglutamine by human tissue. J Biol Chem. 1957 Nov;229(1):463–476. [PubMed] [Google Scholar]
  16. Marcucci F., Airoldi L., Riva R., Mussini E., Garattini S. Effect of estrogen-progestin combinations on the hepatic microsomal metabolism of mestranol. Biochem Pharmacol. 1976 Jun 15;25(12):1339–1344. doi: 10.1016/0006-2952(76)90101-5. [DOI] [PubMed] [Google Scholar]
  17. Matsubara T., Tochino Y. Electron transport systems of lung microsomes and their physiological functions. I. Intracellular distribution of oxidative enzymes in lung cells. J Biochem. 1971 Dec;70(6):981–991. doi: 10.1093/oxfordjournals.jbchem.a129728. [DOI] [PubMed] [Google Scholar]
  18. PHILLIPS A. H., LANGDON R. G. Hepatic triphosphopyridine nucleotide-cytochrome c reductase: isolation, characterization, and kinetic studies. J Biol Chem. 1962 Aug;237:2652–2660. [PubMed] [Google Scholar]
  19. Pandov H., Sims P. The conversion of phenanthrene 9,10-oxide and dibenz[a,h]anthracene 5,6-oxide into dihydrodiols by a rat-liver microsomal enzyme. Biochem Pharmacol. 1970 Jan;19(1):299–303. doi: 10.1016/0006-2952(70)90349-7. [DOI] [PubMed] [Google Scholar]
  20. SCHACHTER D., TAGGART J. V. Glycine N-acylase: purification and properties. J Biol Chem. 1954 May;208(1):263–275. [PubMed] [Google Scholar]
  21. Scherstén T. The synthesis of taurocholic and glycocholic acids by preparations of human liver. I. Distribution of activity between subcellular fractions. Biochim Biophys Acta. 1967 Jun 13;141(1):144–154. [PubMed] [Google Scholar]
  22. Seidegård J., DePierre J. W., Moron M. S., Johannesen K. A., Ernster L. Characterization of rat lung epoxide (styrene oxide) hydrase with a modified radioactive assay of improved sensitivity. Cancer Res. 1977 Apr;37(4):1075–1082. [PubMed] [Google Scholar]
  23. Strahl N. R., Barr W. H. Intestinal drug absorption and metabolism. 3. Glycine conjugation and accumulation of benzoic acid in rat intestinal tissue. J Pharm Sci. 1971 Feb;60(2):278–281. doi: 10.1002/jps.2600600227. [DOI] [PubMed] [Google Scholar]
  24. Tishler S. L., Goldman P. Properties and reactions of salicyl-coenzyme A. Biochem Pharmacol. 1970 Jan;19(1):143–150. doi: 10.1016/0006-2952(70)90335-7. [DOI] [PubMed] [Google Scholar]
  25. Webster L. T., Siddiqui U. A., Lucas S. V., Strong J. M., Mieyal J. J. Identification of separate acyl- CoA:glycine and acyl-CoA:L-glutamine N-acyltransferase activities in mitochondrial fractions from liver of rhesus monkey and man. J Biol Chem. 1976 Jun 10;251(11):3352–3358. [PubMed] [Google Scholar]
  26. Yamashita S., Racker E. Resolution and reconstitution of the mitochondrial electron transport system. II. Reconstitution of succinoxidase from individual components. J Biol Chem. 1969 Mar 10;244(5):1220–1227. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES