Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1978 May 15;172(2):319–325. doi: 10.1042/bj1720319

Changes in the lipoprotein lipase (clearing-factor lipase) activity of white adipose tissue during development of the rat.

A Cryer, H M Jones
PMCID: PMC1185698  PMID: 666749

Abstract

The lipoprotein lipase (clearing-factor lipase) activity of the white adipose tissue from rats aged between 1 and 145 days was determined. Five adipose-tissue sites (epididymal, uterine, subcutaneous, perirenal and intramuscular) together with serum concentrations of triacylglycerol, cholesterol and glucose were studied. The pattern of enzyme-activity change was remarkably similar in all the sites studied, although the growth of the tissues proceeded non-uniformly. After a peak of activity early in suckling, lipoprotein lipase activity fell to low values by 20 days of age. At weaning (21 days) the activity increased sharply and within 5 days high values were regained. The serum triacylglycerol and cholesterol concentrations were low at birth and reached peaks of concentration coincidentally with the minima of white-adipose-tissue lipoprotein lipase activities, seen late in suckling. The changes in enzyme activity were related to other metabolic changes in adipose tissue and with the known changes in plasma insulin concentrations occurring during development.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blázquez E., Montoya E., López Quijada C. Relationship between insulin concentrations in plasma and pancreas of oetal and weanling rats. J Endocrinol. 1970 Dec;48(4):553–561. doi: 10.1677/joe.0.0480553. [DOI] [PubMed] [Google Scholar]
  2. Borensztajn J., Samols D. R., Rubenstein A. H. Effects of insulin on lipoprotein lipase activity in the rat heart and adipose tissue. Am J Physiol. 1972 Dec;223(6):1271–1275. doi: 10.1152/ajplegacy.1972.223.6.1271. [DOI] [PubMed] [Google Scholar]
  3. Carroll K. K., Hamilton R. M., Macleod G. K. Plasma cholesterol levels in suckling and weaned calves, lambs, pigs, and colts. Lipids. 1973 Nov;8(11):635–640. doi: 10.1007/BF02533148. [DOI] [PubMed] [Google Scholar]
  4. Cryer A., Riley S. E., Williams E. R., Robinson D. S. Effect of nutritional status on rat adipose tissue, muscle and post-heparin plasma clearing factor lipase activities: their relationship to triglyceride fatty acid uptake by fat-cells and to plasma insulin concentrations. Clin Sci Mol Med. 1976 Mar;50(3):213–221. doi: 10.1042/cs0500213. [DOI] [PubMed] [Google Scholar]
  5. Cunningham V. J., Robinson D. S. Clearing-factor lipase in adipose tissue. Distinction of different states of the enzyme and the possible role of the fat cell in the maintenance of tissue activity. Biochem J. 1969 Apr;112(2):203–209. doi: 10.1042/bj1120203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DYMSZA H. A., CZAJKA D. M., MILLER S. A. INFLUENCE OF ARTIFICIAL DIET ON WEIGHT GAIN AND BODY COMPOSITION OF THE NEONATAL RAT. J Nutr. 1964 Oct;84:100–106. doi: 10.1093/jn/84.2.100. [DOI] [PubMed] [Google Scholar]
  7. Dodds P. F., Brindley D. N. Is there a biochemical marker for the pre-adipocyte? Biochem Soc Trans. 1976;4(2):229–230. doi: 10.1042/bst0040229. [DOI] [PubMed] [Google Scholar]
  8. Enser M. Clearing-factor lipase in obese hyperglycaemic mice (ob-ob). Biochem J. 1972 Sep;129(2):447–453. doi: 10.1042/bj1290447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FRIEDMAN M., BYERS S. O. Effects of diet on serum lipids of fetal, neonatal, and pregnant rabbits. Am J Physiol. 1961 Oct;201:611–616. doi: 10.1152/ajplegacy.1961.201.4.611. [DOI] [PubMed] [Google Scholar]
  10. Garfinkel A. S., Baker N., Schotz M. C. Relationship of lipoprotein lipase activity to triglyceride uptake in adipose tissue. J Lipid Res. 1967 May;8(3):274–280. [PubMed] [Google Scholar]
  11. Hahn P., Greenberg R., Dobiásová M., Drahota Z. Triglyceride synthesis from various precursors in adipose tissue of the rat during development. Can J Biochem. 1968 Aug;46(8):735–741. doi: 10.1139/o68-113. [DOI] [PubMed] [Google Scholar]
  12. Hahn P., Novak M. Development of brown and white adipose tissue. J Lipid Res. 1975 Mar;16(2):79–91. [PubMed] [Google Scholar]
  13. Hahn P. The postnatal development of hormone sensitive lipase in brown and white adipose tissue of the rat. Experientia. 1965 Nov 15;21(11):634–635. doi: 10.1007/BF02144049. [DOI] [PubMed] [Google Scholar]
  14. Hamilton R. M., Carroll K. K. Plasma cholesterol levels in suckling and weaned kittens, puppies, and guinea pigs. Lipids. 1977 Feb;12(2):145–148. doi: 10.1007/BF02533284. [DOI] [PubMed] [Google Scholar]
  15. Hamosh M., Clary T. R., Chernick S. S., Scow R. O. Lipoprotein lipase activity of adipose and mammary tissue and plasma triglyceride in pregnant and lactating rats. Biochim Biophys Acta. 1970 Sep 8;210(3):473–482. doi: 10.1016/0005-2760(70)90044-5. [DOI] [PubMed] [Google Scholar]
  16. Harding P. G. The metabolism of brown and white adipose tissue in the fetus and newborn. Clin Obstet Gynecol. 1971 Sep;14(3):685–709. doi: 10.1097/00003081-197109000-00004. [DOI] [PubMed] [Google Scholar]
  17. Harris R. A., MacNintch J. E., Quackenbush F. W. Mechanism of suckling rat hypercholesterolemia: dietary and drug studies. J Nutr. 1966 Sep;90(1):40–46. doi: 10.1093/jn/90.1.40. [DOI] [PubMed] [Google Scholar]
  18. Hollenberg C. H., Vost A. Regulation of DNA synthesis in fat cells and stromal elements from rat adipose tissue. J Clin Invest. 1969 Nov;47(11):2485–2498. doi: 10.1172/JCI105930. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Holm G., Jacobsson B., Björntorp P., Smith U. Effects of age and cell size on rat adipose tissue metabolism. J Lipid Res. 1975 Nov;16(6):461–464. [PubMed] [Google Scholar]
  20. Jones C. T. Lipid metabolism and mobilization in the guinea pig during pregnancy. Biochem J. 1976 May 15;156(2):357–365. doi: 10.1042/bj1560357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. KESSLER J. I. Effect of diabetes and insulin on the activity of myocardial and adipose tissue lipoprotein lipase of rats. J Clin Invest. 1963 Mar;42:362–367. doi: 10.1172/JCI104722. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Kannan R., Palmquist D. L., Baker N. Contribution of intermuscular fat to lipogenesis from dietary glucose carbon in mice. Biochim Biophys Acta. 1976 May 27;431(2):225–232. doi: 10.1016/0005-2760(76)90142-9. [DOI] [PubMed] [Google Scholar]
  23. Kazdová L., Fábry P., Vrána A. Effect of small doses of insulin in vivo on the proliferation and cellularity of adipose tissue. Diabetologia. 1974 Feb;10(1):77–83. doi: 10.1007/BF00421417. [DOI] [PubMed] [Google Scholar]
  24. Kirtland J., Gurr M. I., Saville G., Widdowson E. M. Occurrence of "pockets" of very small cells in adipose tissue of the guinea pig. Nature. 1975 Aug 28;256(5520):723–724. doi: 10.1038/256723a0. [DOI] [PubMed] [Google Scholar]
  25. LANG C. A. RESPIRATORY ENZYMES IN THE HEART AND LIVER OF THE PRENATAL AND POSTNATAL RAT. Biochem J. 1965 May;95:365–371. doi: 10.1042/bj0950365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Linder C., Chernick S. S., Fleck T. R., Scow R. O. Lipoprotein lipase and uptake of chylomicron triglyceride by skeletal muscle of rats. Am J Physiol. 1976 Sep;231(3):860–864. doi: 10.1152/ajplegacy.1976.231.3.860. [DOI] [PubMed] [Google Scholar]
  27. Martin P. J. A quick inexpensive method for serum triglycerides. Clin Chim Acta. 1975 Jul 9;62(1):79–88. doi: 10.1016/0009-8981(75)90282-x. [DOI] [PubMed] [Google Scholar]
  28. Otway S., Robinson D. S. The significance of changes in tissue clearing-factor lipase activity in relation to the lipaemia of pregnancy. Biochem J. 1968 Feb;106(3):677–682. doi: 10.1042/bj1060677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. PAV J., WENKEOVA J. Significance of adipose tissue lipoprotein lipase. Nature. 1960 Mar 26;185:926–927. doi: 10.1038/185926a0. [DOI] [PubMed] [Google Scholar]
  30. Péquignot-Planche E., De Gasquet P., Boulangé A., Tonnu N. T. Lipoprotein lipase activity at onset of development of white adipose tissue in newborn rats. Biochem J. 1977 Feb 15;162(2):461–463. doi: 10.1042/bj1620461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Radomski M. W., Orme T. Response of lipoprotein lipase in various tissues to cold exposure. Am J Physiol. 1971 Jun;220(6):1852–1856. doi: 10.1152/ajplegacy.1971.220.6.1852. [DOI] [PubMed] [Google Scholar]
  32. Reichl D. Lipoprotein lipase activity in the adipose tissue of rats adapted to controlled feeding schedules. Biochem J. 1972 Jun;128(1):79–87. doi: 10.1042/bj1280079. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Richmond W. Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum. Clin Chem. 1973 Dec;19(12):1350–1356. [PubMed] [Google Scholar]
  34. Rogers M. P., Robinson D. S. Effects of cold exposure on heart clearing factor lipase and triglyceride utilization in the rat. J Lipid Res. 1974 May;15(3):263–272. [PubMed] [Google Scholar]
  35. SCHNELL W., SCHREIER K., WIEDERHOLT I., HARTMANN W. [Research on the developmental physiology of lipid synthesis]. Clin Chim Acta. 1961 Mar;6:229–232. doi: 10.1016/0009-8981(61)90087-0. [DOI] [PubMed] [Google Scholar]
  36. Salaman M. R., Robinson D. S. Clearing-factor lipase in adipose tissue. A medium in which the enzyme activity of tissue from starved rats increases in vitro. Biochem J. 1966 Jun;99(3):640–647. doi: 10.1042/bj0990640. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Scow R. O., Blanchette-Mackie E. J., Smith L. C. Role of capillary endothelium in the clearance of chylomicrons. A model for lipid transport from blood by lateral diffusion in cell membranes. Circ Res. 1976 Aug;39(2):149–162. doi: 10.1161/01.res.39.2.149. [DOI] [PubMed] [Google Scholar]
  38. Uete T., Hori T., Fukutani C. Ultra-micromethods for the biochemical analysis of cutaneous capillary blood. IV. Selection of the glucose oxidase-2,2'-Azino-di-(3-ethyl-benzthiazolin-sulphonic acid-6)diammonium method for measuring blood sugar of neonates. Clin Chim Acta. 1976 Jul 15;70(2):329–332. doi: 10.1016/0009-8981(76)90435-6. [DOI] [PubMed] [Google Scholar]
  39. VILLEE C. A., HAGERMAN D. D. Effect of oxygen deprivation on the metabolism of fetal and adult tissues. Am J Physiol. 1958 Sep;194(3):457–464. doi: 10.1152/ajplegacy.1958.194.3.457. [DOI] [PubMed] [Google Scholar]
  40. Yeung D., Oliver I. T. Factors affecting the premature induction of phosphopyruvate carboxylase in neonatal rat liver. Biochem J. 1968 Jun;108(2):325–331. doi: 10.1042/bj1080325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. de Gasquet P., Péquignot E., Lemonnier D., Alexiu A. Adipose-tissue lipoprotein lipase activity and cellularity in the genetically obese Zucker rat (fa-fa). Biochem J. 1973 Mar;132(3):633–635. doi: 10.1042/bj1320633. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES